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C06 | Biofabrication of a glomerular ex vivo model by stepwise mimicking functional core components 

By combining top-down fabrication methods and bottom up strategies, we will generate a 3D glomerular ex vivo model. Therefore, we will study cellular behavior of glomerular endothelial cells and podocytes in a co-culture system on different sides of an artificial basement membrane. Next, we will mimic the glomerular structure by integrating mesangial spheroids into capillary loops consisting of glomerular endothelial cells, artificial basement membrane and podocytes (bioassembly). Thereby, we will study 3D structure, self-assembly, maturation, paracrine cell interaction and generation of extracellular matrix.

Prof. Dr. Janina Müller-Deile
Dr. Taufiq Ahmad

Camilla Mussoni

Previous academic education: Master’s degree in Biomedical Engineering from Campus Biomedico University of Rome (master thesis title: A combined approach of 3D bioprinting and electrospinning for vascular tissue engineering)
Academic Advisor within SFB TRR225: Dr. Taufiq Ahmad
Location: University Hospital Würzburg, Department for Functional Materials in Medicine and Dentistry
Topic of thesis: Development of 3D glomerulus model by combining top-down and bottom-up biofabrication strategies
Main techniques and methodologies to be used: Electrospinning, Meltelectrowrinting, 3D printing, and Cell culture

Contact: camilla.mussoni@fmz.uni-wuerzburg.de

Twitter: The world needs better models and we are not kidneying around (or are we?). Our @SFB_TRR225 project C06 @CamillaMussoni aims to use #biofabrication techniques to build a #3D model of the #Glomerulus.

Anna Rederer

Previous academic education: M.Sc. Cell- and molecular biology, FAU Erlangen-Nuremberg, M.Sc. and B. Sc. Biology, V. N. Karasin National University of Kharkiv, Ukraine
Academic Advisor within SFB TRR225: Prof. Dr. Janina Müller-Deile
Location: University Hospital Erlangen, Clinic 4
Topic of thesis: Biofabrication of a glomerular ex vivo model by stepwise mimicking functional core components
Main techniques and methodologies to be used: Cell culture, spheroids generation, immune staining, confocal and electron microscopy, time-lapse imaging, Western Blot, qPCR, ELISA

Contact:  Anna.Rederer@uk-erlangen.de

Julia Eichermüller

Previous academic education: M.Sc. Cell and Molecular Biology, FAU Erlangen-Nürnberg, B.Sc. Biology, FAU Erlangen-Nürnberg
Academic Advisor within SFB TRR225: Prof. Dr. Janina Müller-Deile
Location: University Hospital Erlangen, Clinic 4
Topic of thesis: Generation of human glomerular spheroids and filtration barriers in a vascularized milieu as a personalized model for podocytic diseases
Main techniques and methodologies to be used: Cell culture, spheroids generation, immune staining, confocal and electron microscopy

Contact: Julia.eichermueller@uk-erlangen.de

2023

Camilla Mussoni
Biofabrication of a glomerular filtration barrier model through a biomimetic fibrous membrane to mimic functional core components
DGBM Jena on 14th September 2023 (C06)

Camilla Mussoni, A. Rederer, J. Müller-Deile, T. Ahmad
The Glomerular FIltration Barrier: fabrication and testing of a biomimetic in vitro model
ISBF Biofabrication 2023 on 18th September 2023  (C06)

2024

Mussoni C, Rederer A, Müller-Deile J, Ahmad T
Fabrication and testing of a glomerular filtration barrier model
Biofabrication Winter School (Austria) on 10th January 2024

2023

Anna Laptii, Camilla Mussoni, Julian Bauer, Robin Dai, Oliver Friedrich, Iwona Cicha, Taufiq Ahmad, Janina Müller-Deile
Biofabrication of a glomerular 3D model by mimicking its functional core components
MPS World Summit in Berlin, Germany, 2023 (C06)

2022

Camilla Mussoni, Anna Laptii, Janina Müller-Deile, Taufiq Ahmad
SFB/TRR 225 Project C06: Biofabrication of a glomerular ex vivo model by stepwise mimicking functional core components
Symposium “Biofabrication meets Infection” Würzburg on 25-28- September 2022               C06

Camilla Mussoni, Anna Laptii, Janina Müller-Deile, Taufiq Ahmad
Fabrication of an in-vivo model of the Glomerular Filtration Barrier
Meeting of The International Society for Biofabrication 2022, Montecatini Terme (Italy) on 25-28- September 2022             C06

Camilla Mussoni
Fabrication of an in vitro model of the glomerular filtration barrier IVM_071
Meeting of The International Society for Biofabrication 2022, Montecatini Terme (Italy) on 25-28- September 2022   C06

Prof. Dr. Janina Müller-Deile

  • Commission member in the promotion of young scientists of the IZKF-Erlangen

Dr. Taufiq Ahmad