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C06 | Biofabrication of a glomerular ex vivo model by stepwise mimicking functional core components 

By combining top-down fabrication methods and bottom up strategies, we will generate a 3D glomerular ex vivo model. Therefore, we will study cellular behavior of glomerular endothelial cells and podocytes in a co-culture system on different sides of an artificial basement membrane. Next, we will mimic the glomerular structure by integrating mesangial spheroids into capillary loops consisting of glomerular endothelial cells, artificial basement membrane and podocytes (bioassembly). Thereby, we will study 3D structure, self-assembly, maturation, paracrine cell interaction and generation of extracellular matrix.

Prof. Dr. Janina Müller-Deile
Dr. Taufiq Ahmad

Camilla Mussoni

Previous academic education: Master’s degree in Biomedical Engineering from Campus Biomedico University of Rome (master thesis title: A combined approach of 3D bioprinting and electrospinning for vascular tissue engineering)
Academic Advisor within SFB TRR225: Dr. Taufiq Ahmad
Location: University Hospital Würzburg, Department for Functional Materials in Medicine and Dentistry
Topic of thesis: Development of 3D glomerulus model by combining top-down and bottom-up biofabrication strategies
Main techniques and methodologies to be used: Electrospinning, Meltelectrowrinting, 3D printing, and Cell culture

Contact: camilla.mussoni@uni-wuerzburg.de

Twitter: The world needs better models and we are not kidneying around (or are we?). Our @SFB_TRR225 project C06 @CamillaMussoni aims to use #biofabrication techniques to build a #3D model of the #Glomerulus.

@Uni_WUE @taufiqbiotech trr225biofab.de/project-c06/

Anna Rederer

Previous academic education: M.Sc. Cell- and molecular biology, FAU Erlangen-Nuremberg, M.Sc. and B. Sc. Biology, V. N. Karasin National University of Kharkiv, Ukraine
Academic Advisor within SFB TRR225: Prof. Dr. Janina Müller-Deile
Location: University Hospital Erlangen, Clinic 4
Topic of thesis: Biofabrication of a glomerular ex vivo model by stepwise mimicking functional core components
Main techniques and methodologies to be used: Cell culture, spheroids generation, immune staining, confocal and electron microscopy, time-lapse imaging, Western Blot, qPCR, ELISA

Contact:  Anna.Rederer@uk-erlangen.de

Julia Eichermüller

Previous academic education: M.Sc. Cell and Molecular Biology, FAU Erlangen-Nürnberg, B.Sc. Biology, FAU Erlangen-Nürnberg
Academic Advisor within SFB TRR225: Prof. Dr. Janina Müller-Deile
Location: University Hospital Erlangen, Clinic 4
Topic of thesis: Generation of human glomerular spheroids and filtration barriers in a vascularized milieu as a personalized model for podocytic diseases
Main techniques and methodologies to be used: Cell culture, spheroids generation, immune staining, confocal and electron microscopy

Contact: Julia.eichermueller@uk-erlangen.de

2025

Bixenmann L, Hauck AV, Ahmad T, Nuhn L. Immunodrug Delivery by Self-Accelerating Degradable Polycarbonate Micelles with Transiently Stable Hemiacetal Ester Side Chains. Macromolecules, doi: 10.1021/acs.macromol.4c02729 (2025). (B05, C06)

Dusi F, Weidemeier T, Mussoni C, Stecher S, Berhanne F, Stahlhut P, Ahmad T, Groll J, Becker M, Blunk T, Bauer-Kreisel P. Combining ASC Spheroids and Melt Electrowritten Scaffolds for a 3D Adipose Model Mimicking Structure and Function of Fat Tissue. Adv. NanoBiomed Res., e202500172. doi: 10.1002/anbr.202500172 (2025). (C02, C06)

Heinze C, Mussoni C, Ryma M, Gergely C, Lamberger Z, Schäfer A, Andelovic K, Stahlhut P, Lang G, Groll J, Ahmad T. Bioassembly of Myoblast Spheroids in Electrofibrillated Scaffolds for 3D Muscle Tissue Biofabrication. Small. doi: 10.1002/smll.202503351 (2025). (C06, A07, B02)

Mussoni C, Rederer A, Stepanenko V, Würthner F, Stahlhut P, Groll J, Schiffer M, Ahmad T, Müller-Deile J. Biofabrication of a Filtration Barrier by Integrating Electrospun Membranes and Flow in a Glomerular Co-Culture. Adv. Healthcare Mater. 14. doi: 10.1002/adhm.202501235 (2025). (C06) (plus COVER PICTURE)

2024

Bixenmann L, Ahmad T, Stephan F, Nuhn L. End-Group Dye-Labeled Poly(hemiacetal ester) Block Copolymers: Enhancing Hydrolytic Stability and Loading Capacity for Micellar (Immuno-)Drug Delivery. Biomacromolecules , doi: 10.1021/acs.biomac.4c01229 (2024). (C06, B05)

Harris D, Groß M, Staebler S , Ebert R , Jürgen S, Boßerhoff AK. Modifying the Glycocalyx of Melanoma Cells via Metabolic Glycoengineering Using N-Acetyl-D-glucosamine Analogues. Cells. doi: 0.3390/cells13221831 (2023). (B05, C03)

Lamberger Z,  Mussoni C, Chicaiza Cabezas N, Heck F, Zwingelberg S, Heilig S,  Ahmad T, Groll J, Lang G. Painting bio: A vector-based method for precise G-code generation across scales in biofabrication. Int J Bioprint, doi:10.36922/ijb.6239 (2024). (A07, B02, B04, C06)

Lamberger Z, Mussoni C, Murenu N, Andrade Mier M, Stahlhut P, Ahmad T, Schaefer N, Villmann C, Zwingelberg S, Groll J, Lang G. Streamlining the Highly Reproducible Fabrication of Fibrous Biomedical Specimens toward Standardization and High Throughput. Adv. Healthcare Mater, doi:10.1002/adhm.202402527 (2024). (A07, C05, C06)

Rindt WD, Krug M, Yamada S, Sennefelder F, Belz L, Cheng WH, Azeem M, Kuric M, Evers M, Leich E, Hartmann TN, Pereira AR, HermannM, Hansmann J, Mussoni C, Stahlhut P, Ahmad T, Yassin MA, Mustafa K, Ebert R, Jundt F. A 3D bioreactor model to study osteocyte differentiation and mechanobiology under perfusion and compressive mechanical loading. Acta Biomaterialia doi: 10.1016/j.actbio.2024.06.041 (2024). (C06, B05)

2023

Rederer A, Rose V, Krüger R, Schmittutz L, Swierzy I, Fischer L, Thievessen I, Bauer J , Friedrich O , Schiffer M, Müller-Deile J. Partner, Neighbor, Housekeeper and Dimension: 3D versus 2D Glomerular Co-Cultures Reveal Drawbacks of Currently Used Cell Culture Models. International Journal of Molecular Science (2023). doi: 10.3390/ijms241210384. (C06, Z02)

2025

Mussoni C
Multispheroid Bioassembly through Meltelectrowriting of a Precisely Arranged FibrousArchitecture for Tissue Models
ISBF Biofabrication 2025, 16th September 2025, Warsaw, Poland

Ahmad T, Mussoni C
Bioassembly of Myoblast Spheroids in Electrofibrillated Scaffolds for 3D Muscle Tissue Biofabrication
ISBF Biofabrication 2025, 15th September 2025, Warsaw, Poland

2024

Rederer A
Biofabrication of an artificial glomerular filtration barrier
Biofabrication 2024, Fukuoka, Japan, 12.11.2024     (C06)

Eichermüller J
Testing of different hydrogel constellations for glomerular 3D co-culture in-vitro to be used for further vascularized rat AV-loop model
Biofabrication 2024, Fukuoka, Japan, 12.11.2024     (C06)

Dusi, Weidemeier, Mussoni, Stahlhut, Stecher, Ahmad, Blunk, Bauer-Keisel
3D adipose tissue model with tunable triglyceride content using melt electrowriting (MEW) scaffols seeded with multicellular spheroids
7th TERMIS World Congress, Seattle, Washington, 25-28 June 2024                         C02, C06

T. Ahmad
Fabrication of nanofibrillar microbundle scaffolds for enhanced myogenic induction in human adipose-derivedstem cells
World Biomaterials Congress 2024, Daegu, 30.05.2024  (C06)

2023

Camilla Mussoni
Biofabrication of a glomerular filtration barrier model through a biomimetic fibrous membrane to mimic functional core components
DGBM Jena on 14th September 2023 (C06)

Camilla Mussoni, A. Rederer, J. Müller-Deile, T. Ahmad
The Glomerular FIltration Barrier: fabrication and testing of a biomimetic in vitro model
ISBF Biofabrication 2023 on 18th September 2023  (C06)

2025

Mussoni C
Multispheroid Bioassembly through Meltelectrowriting of a Precisely Arranged FibrousArchitecture for Tissue Models
ISBF Biofabrication 2025, 14th-17th September 2025, Warsaw, Poland                  C06

Mussoni C, Ahmad T
Fabrication of Muscle-fiber-inspired nanofibrillar scaffolds to modulate myogenesis.
GRC Biomaterials and Tissue Engineering 2025, 27th July 2025, Barcelona, Spain                     C06

Mussoni C, Ahmad T
Functionalized double-geometry meltelectrowritten scaffolds for bone to tendon interface engineering
TERMIS EU 2025, 19th-23rd May 2025, Freiburg                                 C06

2024

Rederer A, Mussoni C, Bauer J, Friedrich O, Ahmad T, Müller-Deile J
Biofabrication of an artificial glomerular filtration barrier
Biofabrication 2024, Fukuoka, Japan, 11.11.2024     (C06, Z02)

Rederer A, Mussoni C, Bauer J, Friedrich O, Ahmad T, Müller-Deile J
Effects of 3D microenvironment and incorporation of biofabricated fibers into glomerular spheroids
Biofabrication 2024, Fukuoka, Japan, 11.11.2024     (C06, Z02)

Mussoni C, Rederer A, Müller-Deile J, Ahmad T
Fabrication and testing of a glomerular filtration barrier model
WI3R Symposium Würzburg, 04.07.2024 (C06)                

Mussoni C, Rederer A, Müller-Deile J, Ahmad T
Fabrication and testing of a glomerular filtration barrier model
TERMIS 2024 World Congress, 04.06.2024 (C06)

Mussoni C, Rederer A, Müller-Deile J, Ahmad T
Fabrication and testing of a glomerular filtration barrier model
Biofabrication Winter School (Austria) on 10th January 2024

2023

Anna Laptii, Camilla Mussoni, Julian Bauer, Robin Dai, Oliver Friedrich, Iwona Cicha, Taufiq Ahmad, Janina Müller-Deile
Biofabrication of a glomerular 3D model by mimicking its functional core components
MPS World Summit in Berlin, Germany, 2023 (C06)

2022

Camilla Mussoni, Anna Laptii, Janina Müller-Deile, Taufiq Ahmad
SFB/TRR 225 Project C06: Biofabrication of a glomerular ex vivo model by stepwise mimicking functional core components
Symposium “Biofabrication meets Infection” Würzburg on 25.-28. September 2022               C06

Camilla Mussoni, Anna Laptii, Janina Müller-Deile, Taufiq Ahmad
Fabrication of an in-vivo model of the Glomerular Filtration Barrier
Meeting of The International Society for Biofabrication 2022, Montecatini Terme (Italy) on 25-28- September 2022             C06

Camilla Mussoni
Fabrication of an in vitro model of the glomerular filtration barrier IVM_071
Meeting of The International Society for Biofabrication 2022, Montecatini Terme (Italy) on 25-28- September 2022   C06

Prof. Dr. Janina Müller-Deile

  • Commission member in the promotion of young scientists of the IZKF-Erlangen

Dr. Taufiq Ahmad

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