C06 | Biofabrication of a glomerular ex vivo model by stepwise mimicking functional core components 

By combining top-down fabrication methods and bottom up strategies, we will generate a 3D glomerular ex vivo model. Therefore, we will study cellular behavior of glomerular endothelial cells and podocytes in a co-culture system on different sides of an artificial basement membrane. Next, we will mimic the glomerular structure by integrating mesangial spheroids into capillary loops consisting of glomerular endothelial cells, artificial basement membrane and podocytes (bioassembly). Thereby, we will study 3D structure, self-assembly, maturation, paracrine cell interaction and generation of extracellular matrix.

Prof. Dr. Janina Müller-Deile
Dr. Taufiq Ahmad

Camilla Mussoni

Previous academic education: Master’s degree in Biomedical Engineering from Campus Biomedico University of Rome (master thesis title: A combined approach of 3D bioprinting and electrospinning for vascular tissue engineering)
Academic Advisor within SFB TRR225: Dr. Taufiq Ahmad
Location: University Hospital Würzburg, Department for Functional Materials in Medicine and Dentistry
Topic of thesis: Development of 3D glomerulus model by combining top-down and bottom-up biofabrication strategies
Main techniques and methodologies to be used: Electrospinning, Meltelectrowrinting, 3D printing, and Cell culture

Contact: camilla.mussoni@fmz.uni-wuerzburg.de

Twitter: The world needs better models and we are not kidneying around (or are we?). Our @SFB_TRR225 project C06 @CamillaMussoni aims to use #biofabrication techniques to build a #3D model of the #Glomerulus.

Anna Laptii

Previous academic education: M.Sc. Cell- and molecular biology, FAU Erlangen-Nuremberg, M.Sc. and B. Sc. Biology, V. N. Karasin National University of Kharkiv, Ukraine
Academic Advisor within SFB TRR225: Prof. Dr. Janina Müller-Deile
Location: University Hospital Erlangen, Clinic 4
Topic of thesis: Biofabrication of a glomerular ex vivo model by stepwise mimicking functional core components
Main techniques and methodologies to be used: Cell culture, spheroids generation, immune staining, confocal and electron microscopy, time-lapse imaging, Western Blot, qPCR, ELISA

Contact: Anna.Laptii@uk-erlangen.de