C03 | Analysis of tumor dormancy and progression in biofabricated vascularized 3D models

The aim of the second funding period is the in vitro biofabrication of in vivo tumor models as well as in vivo evaluation of tumor models in the vascularized AV loop model. The primary focus will be the evaluation of tumor dormancy and progression and the influence of the tumor microenvironment. Based on our previous studies, more complex tumor models will be biofabricated using different areas, stiffnesses and cell types and the tumor cell behavior will be analyzed also on molecular level.

Prof. Dr. Andreas Arkudas
Prof. Dr. Anja Boßerhoff
PD Dr. Annika Kengelbach-Weigand

Rafael Schmid

Previous academic education: Master of Tissue Engineering and Regenerative Medicine
Academic Advisor within SFB TRR225: Prof. Dr. Andreas Arkudas, Dr. Annika Kengelbach-Weigand
Location: University Hospital of Erlangen, Department of Plastic and Hand Surgery
Topic of thesis: Analysis of tumor dormancy and progression in biofabricated and vascularized in vivo 3D models
Main techniques and methodologies to be used: cell culture, cell culture assays, (live cell) microscopy, in vivo AV loop, in vivo 3D imaging (immuno)histology, gene expression

Contact: rafael.schmid@uk-erlangen.de

Alexander Deuschel

Previous academic education: Master of Science in Biochemistry at the University of Würzburg
Academic Advisor within SFB TRR225: Prof. Dr. Anja Bosserhoff
Location: FAU Erlangen-Nürnberg, Department for Biochemistry and Molecular Medicine
Topic of thesis: Analysis of tumor dormancy and progression in biofabricated and vascularized in vivo 3D models
Main techniques and methodologies to be used: Cell culture, qPCR, fluorescence microscopy, FACS, Western Blot

Contact: alex.deuschel@fau.de

Tannaz Karimi

Previous academic education: Master of Biomedical Engineering/ Biomaterials
Academic Advisor within SFB TRR225: PD Dr. Annika Kengelbach-Weigand, Prof. Dr. Andreas Arkudas, Prof. Dr. Anja Bosserhoff,
Location: University Hospital of Erlangen, Department of Plastic and Hand Surgery
Topic of thesis: Analysis of tumor dormancy and progression in biofabricated vascularized 3D models
Main techniques and methodologies to be used: cell culture, cell culture assays, (live cell) microscopy, in vivo AV loop, in vivo 3D imaging (Immuno)histology

Contact:

Ertekin O, Monavari M, Kruger R, Fuentes-Chandia M, Parma B, Letort G, Tripal P, Boccaccini AR, Bosserhoff AK, Ceppi P, Kappelmann-Fenzl M, Leal-Egana A, 3D hydrogel-based microcapsules as an in vitro model to study tumorigenicity, cell migration and drug resistance, Acta Biomater 142 (2022) 208-220. (C03)

Sivakumar S, Schmid R, Wieland A, Strissel PL, Strick R, Fischer L, Thievessen I, Kataev E, Arkudas A, Horch RE, Schubert DW, Kengelbach‐Weigand A, Role of Fiber Thickness and Surface Treatment of Electrospun Polycaprolactone Matrices on the Growth of Different Breast Cancer‐Associated Cells, Advanced Materials Interfaces doi:10.1002/admi.202101808  (2022). (B01, B06, C03)

Sörgel CA, Schmid R, Stadelmann N, Weisbach V, Distel L, Horch RE, Kengelbach-Weigand A, IGF-I and Hyaluronic Acid Mitigate the Negative Effect of Irradiation on Human Skin Keratinocytes, Cancers 14(3) (2022). (C03, C04)

Kappelmann-Fenzl M, Schmidt SK, Fischer S, Schmid R, Lammerhirt L, Fischer L, Schrufer S, Thievessen I, Schubert DW, Matthies A, Detsch R, Boccaccini AR, Arkudas A, Kengelbach-Weigand A, Bosserhoff AK, Molecular Changes Induced in Melanoma by Cell Culturing in 3D Alginate Hydrogels, Cancers (Basel) 13(16) (2021). (C03, A01, A07, B06)

Schmid R, Schmidt SK, Detsch R, Horder H, Blunk T, Schrüfer S, Schubert DW, Fischer L, Thievessen I, Heltmann‐Meyer S, Steiner D, Schneidereit D, Friedrich O, Grüneboom A, Amouei H, Wajant H, Horch RE, Bosserhoff AK, Arkudas A, Kengelbach‐Weigand A, A New Printable Alginate/Hyaluronic Acid/Gelatin Hydrogel Suitable for Biofabrication of In Vitro and In Vivo Metastatic Melanoma Models, Adv. Funct. Mater. doi:10.1002/adfm.202107993 (2021). (C03, A01, A07, B06, B08, C02, C04)

Winkler S, Mutschall H, Biggemann J, Fey T, Greil P, Korner C, Weisbach V, Meyer-Lindenberg A, Arkudas A, Horch RE, Steiner D, Human Umbilical Vein Endothelial Cell Support Bone Formation of Adipose-Derived Stem Cell-Loaded and 3D-Printed Osteogenic Matrices in the Arteriovenous Loop Model, Tissue Eng Part A 27(5-6) (2021) 413-423. (C04, C03)

Fuentes-Chandia M, Vierling A, Kappelmann-Fenzl M, Monavari M, Letort G, Hone L, Parma B, Antara SK, Ertekin O, Palmisano R, Dong M, Bopple K, Boccaccini AR, Ceppi P, Bosserhoff AK, Leal-Egana A, 3D Spheroids Versus 3D Tumor-Like Microcapsules: Confinement and Mechanical Stress May Lead to the Expression of Malignant Responses in Cancer Cells, Adv. Biol. doi:10.1002/adbi.202000349  (2021) 2000349. (C03, A01)

Tolksdorf J, Horch RE, Gruner JS, Schmid R, Kengelbach-Weigand A, Schubert DW, Werner S, Schneidereit D, Friedrich O, Ludolph I, Size matters-in vitro behaviour of human fibroblasts on textured silicone surfaces with different pore sizes, J. Mater. Sci.: Mater. Med. 31(2) (2020) 23. (C04, B08, A07, C03)

Schmid R, Schmidt SK, Hazur J, Detsch R, Maurer E, Boccaccini AR, Hauptstein J, Tessmar J, Blunk T, Schrufer S, Schubert DW, Horch RE, Bosserhoff AK, Arkudas A, Kengelbach-Weigand A, Comparison of Hydrogels for the Development of Well-Defined 3D Cancer Models of Breast Cancer and Melanoma, Cancers 12(8) (2020) 2320. (C03, A01, A02, A07, C02)

Mutschall H, Winkler S, Weisbach V, Arkudas A, Horch RE, Steiner D, Bone tissue engineering using adipose-derived stem cells and endothelial cells: Effects of the cell ratio, J. Cell. Mol. Med. 24(12) (2020) 7034-7043. (C04, C03)

Bohme I, Bosserhoff A, Extracellular acidosis triggers a senescence-like phenotype in human melanoma cells, Pigm. Cell Melanoma Res. 33(1) (2020) 41-51. (C03)

Steiner D, Lang G, Fischer L, Winkler S, Fey T, Greil P, Scheibel T, Horch RE, Arkudas A, Intrinsic Vascularization of Recombinant eADF4(C16) Spider Silk Matrices in the Arteriovenous Loop Model, Tissue Eng Part A 25(21-22) (2019) 1504-1513. (C04, A07, C01, C03)

Schmidt SK, Schmid R, Arkudas A, Kengelbach-Weigand A, Bosserhoff AK, Tumor Cells Develop Defined Cellular Phenotypes After 3D-Bioprinting in Different Bioinks, Cells 8(10) (2019) 1295. (C03)

Kengelbach-Weigand A, Tasbihi K, Strissel PL, Schmid R, Marques JM, Beier JP, Beckmann MW, Strick R, Horch RE, Boos AM, Plasticity of patient-matched normal mammary epithelial cells is dependent on autologous adipose-derived stem cells, Sci. Rep. 9(1) (2019) 10722. (C03)

Weigand A, Horch RE, Boos AM, Beier JP, Arkudas A, The Arteriovenous Loop: Engineering of Axially Vascularized Tissue, Eur. Surg. Res. 59(3-4) (2018) 286-299. (C03, C04)

Steiner D, Lingens L, Fischer L, Kohn K, Detsch R, Boccaccini AR, Fey T, Greil P, Weis C, Beier JP, Horch RE, Arkudas A, Encapsulation of Mesenchymal Stem Cells Improves Vascularization of Alginate-Based Scaffolds, Tissue Eng Part A 24(17-18) (2018) 1320-1331. (C03, C04, A01, B06, B03) Front Cover for Tissue Engineering Part A

Rottensteiner-Brandl U, Detsch R, Sarker B, Lingens L, Kohn K, Kneser U, Bosserhoff AK, Horch RE, Boccaccini AR, Arkudas A, Encapsulation of Rat Bone Marrow Derived Mesenchymal Stem Cells in Alginate Dialdehyde/Gelatin Microbeads with and without Nanoscaled Bioactive Glass for In Vivo Bone Tissue Engineering, Materials (Basel) 11(10) (2018) 1880. (C04, A01, C03)

An R, Schmid R, Klausing A, Robering JW, Weber M, Bauerle T, Detsch R, Boccaccini AR, Horch RE, Boos AM, Weigand A, Proangiogenic effects of tumor cells on endothelial progenitor cells vary with tumor type in an in vitro and in vivo rat model, FASEB J. 32(10) (2018) 5587-5601. (C03)

Schmid R, Schmidt S, Hazur J, Detsch R, Boccaccini AR, Bosserhoff A, Horch RE, Arkudas A, Kengelbach-Weigand A (2019) Analyse von Tumor Dormancy und Progression in biofabrizierten und in vivo vaskularisierten 3D Modellen; 50. Jahrestagung der Deutschen Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen (DGPRÄC) September 26-28, 2019 (Hamburg, Germany)

Kengelbach-Weigand A (2019) In vitro und in vivo Modelle zur Erforschung der Mammakarzinompathogenese; 50. Jahrestagung der Deutschen Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen (DGPRÄC) September 26-28, 2019 (Hamburg, Germany)

Kengelbach-Weigand A, Schmid R, Tasbihi K, Strissel PL, Strick R, Al-Abboodi M, An R, Robering JW, Eckstein M, Bäuerle T, Detsch R, Boccaccini AR, Dalton PD, Youssef A, Wolf K, Kremer AE, Boos AM, Bosserhoff A, Arkudas A, Horch RE (2019) The arteriovenous loop as 3D model for studying the interaction of ADSC and breast cancer cells, TERMIS EU Workshop 2019, 26th-27th Aug 2019, Nantes, France

Kengelbach-Weigand A (2019) Das arteriovenöse Gefäßschleifenmodell als Tool für die Untersuchung der Interaktion von ADSC und Mammakarzinomzellen, 3. DAM Wissenschaftsakademie, 12 – 13 April 2019 (Erlangen, Germany)

Schmid R (2019) Analyse von Tumor dormancy und Progression in biofabrizierten und in vivo vaskularisierten 3D-Modellen, 3. DAM Wissenschaftsakademie, 12 – 13 April 2019 (Erlangen, Germany)

Weigand A, Boos AM, An R, Strissel PL, Schmid R, Robering JW, Al-Abboodi M, Detsch R, Dalton PD, Youssef A , Boccaccini AR, Beckmann MW, Bäuerle T, Strick R, Horch RE (2018) Vom Tissue Engineering zum Tumor Engineering, 49. Jahrestagung der Deutsche Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen DGPRÄC & 23. Jahrestagung der Vereinigung der Deutschen Ästhetisch Plastischen Chirurgen (VDÄPC), 13 – 15 September 2018 (Bochum, Germany)

Weigand A, Beier JP, Schmid R, Robering JW, Cai A, Arkudas A, Horch RE, Boos A (2018) Vascularized Tissue Engineering Using the Arteriovenous Loop – The Path from the Small Animal Model to the Preclinical Large Animal Model, 2018 TERMIS World Congress, 4-7 September 2018 (Kyoto, Japan)

Peddi A, Steiner D, Weigand A, Lingens L, Fischer L, Köhn K, Detsch R, Boccaccini AR, Fey T, Greil P, Weis C, Beier JP, Horch RE, Arkudas A. (2018) Encapsulation of mesenchymal stem cells improves vascularization of alginate-based scaffolds,  The 10th EPSRC Meeting, 23 – 25 August 2018 (Hamburg, Germany)

Kengelbach-Weigand A, Schmid R, Schmidt SK, Hazur J, Detsch R, Maurer E, Boccaccini AR, Hauptstein J, Teßmar J, Blunk T, Schrüfer S, Schubert DW, Horch RE, Bosserhoff AK, Arkudas A (2021) Entwicklung eines biofabrizierten 3D-Tumormodells, 51. Jahrestagung der DGPRÄC, 25. Jahrestagung der VDÄPC, Oktober 14-16 (virtuell)

Sivakumar S, Schmid R, Wieland A, Strissel PL, Strick R, Fischer L, Thievessen I, Kataev E, Arkudas A, Horch RE, Schubert DW, Kengelbach-Weigand A, “Role of Fiber Thickness and Surface Treatment of Electrospun Polycaprolactone Matrices on the Growth of Different Breast Cancer-Associated Cells” 31st Annual SBAOI meeting and 14th STERMI annual meeting and International conference on Biomaterials-based therapeutics, engineering and medicine (BIOTEM-2021), December 17-20, 2021.

Schmidt SK, Kappelmann-Fenzl M, Fischer S, Schmid R, Lämmerhirt L, Fischer L, Schrüfer S, Thievessen I, Schubert DW, Matthies A, Detsch R, Boccaccini AR, Arkudas A, Kengelbach-Weigand A, Bosserhoff AK (2021) Analyse molekularer Veränderungen im Melanom, induziert durch Zellkultivierung in 3D Alginat-Hydrogelen, 31. Deutscher Hautkrebskongress der Arbeitsgemeinschaft Dermatologische Onkologie (ADO), 08.-11. September 2021

Schmid R, Schmidt SK, Detsch R, Horder H, Blunk T, Schrüfer S, Schubert DW, Fischer L, Thievessen I, Heltmann-Meyer S, Steiner D, Schneidereit D, Friedrich O, Grüneboom A, Amouei H, Wajant H, Horch RE, Bosserhoff AK, Arkudas A, Kengelbach-Weigand A (2021) A new printable alginate / hyaluronic acid / gelatin hydrogel suitable for biofabrication of in vitro and in vivo metastatic melanoma models, 31st Annual Conference of the European Society for Biomaterials (ESB 2021), 5 to 9 September 2021 (C03, A01, A07, B06, B08, C02, C04)

Schmidt SK, Kappelmann-Fenzl M, Fischer S, Schmid R, Lämmerhirt L, Fischer L, Schrüfer S, Thievessen I, Schubert DW, Matthies A, Detsch R, Boccaccini AR, Arkudas A, Kengelbach-Weigand A, Bosserhoff AK (2021) Molecular changes induced in melanoma by cell culturing in 3D alginate hydrogels. 31st Annual Conference of the European Society for Biomaterials (ESB 2021), 5.-9. September 2021

Schmidt SK, Schmid R, Arkudas A, Kengelbach-Weigand A, Bosserhoff AK (2020) 3D-Druck von Melanomzellen mit kommerziell erwerblichen Hydrogelen, Digitaler 30. Deutscher Hautkrebskongress der Arbeitsgemeinschaft Dermatologische Onkologie (ADO), 10.-11. September 2020

Schmid R, Schmidt SK, Hazur J, Detsch R, Boccaccini AR, Horch RE, Bosserhoff AK, Arkudas A, Kengelbach-Weigand A (2019) Comparison of hydrogels for the development of well defined 3D cancer models of breast cancer and melanoma, 4th EACR Conference on Goodbye Flat Biology, Berlin, Germany, 10.-13. November 2019

Schmidt SK, Schmid R, Arkudas A, Kengelbach-Weigand A, Bosserhoff AK (2019) 3D-Bioprinting of melanoma cells with commercially available matrices, 4th EACR Conference on Goodbye Flat Biology, Berlin, Germany, 10.-13. November 2019

Weigand A, Boos AM, An R, Strissel PL, Schmid R, Robering JW, Al-Abboodi M, Detsch R, Dalton PD, Youssef A , Boccaccini AR, Beckmann MW, Bäuerle T, Strick R, Horch RE (2018) From Tissue Engineering to Tumor Engineering –  Application of the arteriovenous loop model for the generation of a fully vascularized tumor in an animal model, 2018 TERMIS World Congress, 4-7 September 2018 (Kyoto, Japan)

Ajay Peddi

Previous academic education: Master in Biomedical Engineering, Martin Luther University Halle, Germany Effects of Fresh Blood Versus Old Blood (stored) on Hepatocyte Viability, Proliferation, Gene Expression; Comparison of the Effects in Hepatocyte 2D and 3D Cell Culture Models
Bachelor of Pharmacy, Rajeev Gandhi University of Health Sciences, India
Academic Advisor within SFB TRR225: Prof. Dr. Andreas Arkudas, Dr. Annika Weigand
Location: University Hospital of Erlangen, Department of Plastic and Hand Surgery
Topic of thesis: Analysis of tumor dormancy and progression in biofabricated and vascularized in vivo 3D models
Main techniques and methodologies to be used: cell culture, cell culture assays, (live cell) microscopy, in vivo AV loop, in vivo 3D imaging (immuno)histology, gene expression

Sonja Schmidt

Previous academic education: Master of Science
Academic Advisor within SFB TRR225: Prof. Dr. Anja Boßerhoff
Location: FAU Erlangen-Nürnberg, Department for Biochemistry and Molecular Medicine
Topic of thesis: Analysis of tumor dormancy and progression in biofabricated and in vivo vascularized 3D models
Main techniques and methodologies to be used: Analysis of cell behavior & signaling pathways (cell culture, fluorescence microscopy, qPCR, Western Blot, …)

Contact: sonja.s.schmidt@fau.de