B09 | Biofabricated gradients for functional tissue models

The aim of this project is to develop a platform technology to biofabricate defined and reproducible gradients in space and time, to analyze them and to model them in silico in order to be able to investigate their effects on cell-biomaterial interactions. For this purpose, we will first develop print heads that enable us to print controlled transitions of materials from the A / B projects, active substances and cells. Through the comprehensive characterization of the printed gradients using mechanical testing methods in combination with image processing, we will constantly analyze and improve the result with regard to the requirements of the C projects. In addition, we will use continuum mechanics modeling and simulation to systematically optimize process parameters, the print pattern and the 3D arrangement within the construct.

Dr.-Ing. Silvia Budday
Prof. Dr. Tomasz Jüngst

 

Dr.-Ing. Silvia Budday

FAU Erlangen, Institute of Applied Mechanics
Contact: silvia.budday@fau.de

Scientific Leadership: Boards, Committees

Jun-Prof. Dr. Tomasz Jüngst

University Hospital Würzburg, Department for Functional Materials in Medicine and Dentistry
Contact: tomasz.juengst@fmz.uni-wuerzburg.de

Scientific Leadership: Boards, Committees

Jessica Faber

Previous academic education: M.Sc. in Mechanical Engineering and M.Sc. in Industrial Engineering and Management
Academic Advisor within SFB TRR225: Dr.-Ing. Silvia Budday
Location: FAU Erlangen, Institute of Applied Mechanics
Topic of thesis: Complex mechanical analysis and modeling of spatio-temporal gradients in biofabricated constructs
Main techniques and methodologies to be used: Multi-modal large-strain mechanical testing (compression, tension, shear, indentation), continuum mechanics modeling, Finite Element (FE) simulations

Contact: jessica.faber@fau.de

Florian Hofmann

Previous academic education: Diploma-Thesis
Academic Advisor within SFB TRR225: Dr. Tomasz Jüngst
Location: University of Würzburg, Department for functional materials in medicine and dentistry
Topic of thesis: Microfluidic chips for creating gradients of bioinks through extrusion-based bioprinting
Main techniques and methodologies to be used: SLA/DLP, Extrusion-based 3D-Bioprinting, Microfluidics

Contact: florian.hofmann@fmz.uni-wuerzburg.de

2023

Genc H, Cianciosi A, Lohse R, Stahlhut P, Groll J, Alexiou C, Cicha I, Jungst T, Adjusting Degree of Modification and Composition of gelAGE-Based Hydrogels Improves Long-Term Survival and Function of Primary Human Fibroblasts and Endothelial Cells in 3D Cultures, Biomacromolecules doi:10.1021/acs.biomac.2c01536  (2023). (B02, B09)

2022

Janzen D, Bakirci E, Faber J, Mier MA, Hauptstein J, Pal A, Forster L, Hazur J, Boccaccini AR, Detsch R, Tessmar J, Budday S, Blunk T, Dalton PD, Villmann C, Reinforced Hyaluronic acid-based Matrices promote 3D Neuronal Network Formation, Adv Healthc Mater doi:10.1002/adhm.202201826  (2022) e2201826. (A01, A02, B06, B09, B01/C05)

Mehnert M, Faber J, Hossain M, Chester SA, Steinmann P, Experimental and numerical investigation of the electro-mechanical response of particle filled elastomers – Part I: Experimental investigations, European Journal of Mechanics – A/Solids 96 (2022). (B09)

Mehnert M, Faber J, Hossain M, Chester SA, Steinmann P, Experimental and numerical investigations of the electro-mechanical response of particle filled elastomers—Part II: Continuum modeling approach, European Journal of Mechanics – A/Solids 96 (2022). (B09)

Faber J, Hinrichsen J, Greiner A, Reiter N, Budday S, Tissue-Scale Biomechanical Testing of Brain Tissue for the Calibration of Nonlinear Material Models, Curr Protoc 2(4) (2022) e381. (B09)

Published Preparatory Work (Project B09 related)

Pien N, Bartolf‐Kopp M, Parmentier L, Delaey J, De Vos L, Mantovani D, Van Vlierberghe S, Dubruel P, Jungst T, Melt Electrowriting of a Photo‐Crosslinkable Poly( ε ‐Caprolactone)‐Based Material into Tubular Constructs with Predefined Architecture and Tunable Mechanical Properties, Macromolecular Materials and Engineering doi:10.1002/mame.202200097  (2022). (Z01) Publication image has been selected as the journal cover of the July 2022 issue of Macro Molecular Materials and Engineering

Fischhaber N, Faber J, Bakirci E, Dalton PD, Budday S, Villmann C, Schaefer N, Spinal Cord Neuronal Network Formation in a 3D Printed Reinforced Matrix-A Model System to Study Disease Mechanisms, Adv Healthc Mater doi:10.1002/adhm.202100830  (2021) e2100830. (B01, Z01)Levato R, Jungst T, Scheuring RG, Blunk T, Groll J, Malda J, From Shape to Function: The Next Step in Bioprinting, Adv Mater 32(12) (2020) 1906423. (A02, B04, Z01)

Hauptstein J, Bock T, Bartolf-Kopp M, Forster L, Stahlhut P, Nadernezhad A, Blahetek G, Zernecke-Madsen A, Detsch R, Jungst T, Groll J, Tessmar J, Blunk T, Hyaluronic Acid-Based Bioink Composition Enabling 3D Bioprinting and Improving Quality of Deposited Cartilaginous Extracellular Matrix, Adv Healthc Mater 9(15) (2020) 2000737. (A02, A06, B02, B06, Z01)

Levato R, Jungst T, Scheuring RG, Blunk T, Groll J, Malda J, From Shape to Function: The Next Step in Bioprinting, Adv Mater 32(12) (2020) 1906423. (A02, B04, Z01)

2022

Tomasz Jungst  Developing tissue models to study vascular diseases      Meeting of The International Society for Biofabrication 2022, Montecatini Terme (Italy) on 25-28- September 2022 https://www.biofabrication2022.org/                             B09

Jessica Faber     Tissue-scale biomechanical testing of brain tissue
for the calibration of nonlinear material models               GAMM 2022 – 92nd GAMM Annual Meeting of the International Association of Applied Mathematics and Mechanics                            B09

2022

Jessica Faber, Florian Hofmann, Silvia Budday, Tomasz Jüngst    SFB/TRR 225 Project B09: Biofabricated gradients for functional tissue models              Symposium “Biofabrication meets Infection” Würzburg on 25-28- September 2022                B09

Florian Hofmann              Microfluidic printheads for multi-material mixing NTB_058         Meeting of The International Society for Biofabrication 2022, Montecatini Terme (Italy) on 25-28- September 2022 https://www.biofabrication2022.org/   B09

Jun-Prof. Dr. Tomasz Jüngst

• Spokesperson of the Young Scientists of the German Society for Biomaterials

Dr. Silvia Budday

• Member of the Programme Committee of the DFG’s SPP 2311
• Deputy Speaker of the Computational Biomechanics Committee of GAMM
• Women’s Representative of the Department Mechanical Engineering of FAU
• Associate Editor of the journal Computer Methods in Biomechanics and Biomedical Engineering, member of Editorial Board of the journal Scientific Reports, Guest co-editor of the journals Frontiers in Mechanical Engineering and Frontiers in Bioengineering and Biotechnology for the Special Issue “Advances in Brain Mechanics