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B09 | Biofabricated gradients for functional tissue models

The aim of this project is to develop a platform technology to biofabricate defined and reproducible gradients in space and time, to analyze them and to model them in silico in order to be able to investigate their effects on cell-biomaterial interactions. For this purpose, we will first develop print heads that enable us to print controlled transitions of materials from the A / B projects, active substances and cells. Through the comprehensive characterization of the printed gradients using mechanical testing methods in combination with image processing, we will constantly analyze and improve the result with regard to the requirements of the C projects. In addition, we will use continuum mechanics modeling and simulation to systematically optimize process parameters, the print pattern and the 3D arrangement within the construct.

Prof. Dr. Silvia Budday
Jun. Prof. Dr. Tomasz Jüngst

 

Jessica Faber

Previous academic education: M.Sc. in Mechanical Engineering and M.Sc. in Industrial Engineering and Management
Academic Advisor within SFB TRR225: Dr.-Ing. Silvia Budday
Location: FAU Erlangen, Institute of Applied Mechanics
Topic of thesis: Complex mechanical analysis and modeling of spatio-temporal gradients in biofabricated constructs
Main techniques and methodologies to be used: Multi-modal large-strain mechanical testing (compression, tension, shear, indentation), continuum mechanics modeling, Finite Element (FE) simulations

Contact: jessica.faber@fau.de

Florian Hofmann

Previous academic education: Diploma-Thesis
Academic Advisor within SFB TRR225: Dr. Tomasz Jüngst
Location: University of Würzburg, Department for functional materials in medicine and dentistry
Topic of thesis: Microfluidic chips for creating gradients of bioinks through extrusion-based bioprinting
Main techniques and methodologies to be used: SLA/DLP, Extrusion-based 3D-Bioprinting, Microfluidics

Contact: florian.hofmann@fmz.uni-wuerzburg.de

2025

Andrade Mier MS, Türker E, Faber J, Friedrich M, Lamberger Z, Weigelt J, Suwannakot P, Gantert B, Singh A, Moessler V, Sodmann A, Murenu N, Schenk J, Schaefer N, Blunk T, Boccaccini AR, Lühmann TC, Tessmar J, Crook JM, Tomaskovic-Crook E, Dalton PD, Lang G, Blum R, Strick R, Budday S, Heinze KG, Villmann C. 3D In Vitro Glioma‐Neuron‐Astrocyte Biomimetic Composites Recapitulate Key Molecular Mechanisms Linked to Glioblastoma Multiforme Pathophysiology. Advanced Functional Materials, doi: 10.1002/adfm.202419211 (2025). (A01, A02, A07, B06, B09, C02, C05, Z02)

Eckert C, Schmidt S, Faber J, Detsch R, Vielreicher M, Lamberger Z, Stahlhut P, Sandor E, Karimi T, Schmid R, Arkudas A, Friedrich O, Budday S, Lang G, Kengelbach-Weigand A, Bosserhoff A. An alginate-cellulose based bioink mimics the viscoelastic features of the melanoma microenvironment and its influence on cell cycle and invasion. Bioprinting, 46, doi: 10.1016/j.bprint.2024.e00384 (2025). (A07, B06, B09, C03, Z02, Z03).

Faber J, Hinrichsen J, Soufivand AA, Lu HH, Rosenberger T, Karakaya E,  Detsch R, Boccaccini AR, Budday S. Tuning the mechanical properties of alginate dialdehyde-gelatin (ADA-GEL) bioinks for bioprinting approaches by varying the degree of oxidation. J Mech Behav Biomed Mater, 163, doi: 10.1016/j.jmbbm.2024.106871 (2025). (B09, B03, B06)

Gantert B, Karakaya E, Hofmann F, Jungst T, Bosserhoff AK, Meinel L, Detsch R, Lühmann T. Alginate-Dialdehyde-Based Reporter Ink Enabling Online Detection of Matrix Metalloproteinase Activity of Encapsulated Cells. ACS Biomater Sci Eng, doi: 10.1021/acsbiomaterials.4c02399 (2025). (B06, C03, Z03, B09)

Kriukov K, Schneider D, Zeck S, Hahn L, Hofmann F, Luxenhofer R, Ebert R. Assessment of viability and mechanoresponsiveness of hMSC-TERT printed with bioinert, thermoresponsive hydrogels. Scientific Reports volume 15, 12257. doi: 10.1038/s41598-025-97196-9 (2025). (A03, B05, B09)

2024

Böhringer D, Cóndor M, Bischof L, Czerwinski T,  Gampl N, Ngo PA, Bauer A, Voskens C, López-Posadas R, Franze K, Budday S, Mark C, Fabry B, Gerum R. Dynamic traction force measurements of migrating immune cells in 3D biopolymer matrices. Nature Physics, doi: 10.1038/s41567-024-02632-8 (2024). (A01, B09,C02)

Cianciosi A, Pfeiffle M, Wohlfahrt P, Nürnberger S, Jungst T. Optical Fiber-Assisted Printing: A Platform Technology for Straightforward Photopolymer Resins Patterning and Freeform 3D Printing. bioRxiv, doi: 10.1101/2024.01.17.576081 (2024). (B09, C02)

Doryab A, Braig J, Jungst T, Ryma M, Groll J. Fabrication Strategies for Bioinspired and Functional Lung-on-Chips. Adv. Funct. Mater. doi: 10.1002/adfm.20240772 (2024). (B02, B04, B09)

Horder H, Böhringer D, Endrizzi N, Hildebrand LS, Cianciosi A, Stecher S, DusiF, Schweinitzer S, Watzling M, Groll J,  Jüngst T, Teßmar L, Bauer-Kreisel P, Fabry B, Torsten B. Cancer cell migration depends on adjacent ASC and adipose spheroids in a 3D bioprinted breast cancer model. Biofabrication doi: 10.1088/1758-5090/ad57f7 (2024). (C02, A01, A02, B09)

Karakaya E, Gleichauf L, Schöbel L, Hassan A, Soufivand AA, Tessmar J, Budday S,  Boccaccini AR, Detsch R. Engineering peptide-modified alginate-based bioinks with cell-adhesive properties for biofabrication. RSC Adv. doi: 10.1039/D3RA08394B. (2024). (B06, A02, A01, B09)

Kellnberger R, Jüngst T, Gekle S. Novel lattice Boltzmann method for simulation of strongly shear thinning viscoelastic fluids. Int J Numer Meth Fluids. doi: 10.1002/fld.5335 (2024). (B07, B09, Z01)

Türker E, Andrade Mier MS, Faber J, Padilla Padilla SJ, Murenu N, Stahlhut P, Lang G, Lamberger Z, Weigelt J, Schaefer N, Tessmar J, Strissel PL, Blunk T, Budday S, Strick R, Villmann C. Breast tumor cell survival and morphology in a brain-like extracellular matrix depends onmatrix composition and mechanical properties. Adv Biol doi: 10.1002/adbi.202400184. (2024) (A02, A07, B09, C02, C05)

2023

Ahmadi Soufivand A, Faber J, Hinrichsen J, Budday S, Multilayer 3D bioprinting and complex mechanical properties of alginate-gelatin mesostructures, Sci Rep 13(1) (2023) 11253. (B09)

Böhringer D, Bauer A, Moravec I, Bischof L, Kah D, Mark C, Grundy TJ, Görlach E, Neill GMO, Budday S, Strissel P, Strick R, Malandrino A, Mak M, Rausch M, Fabry B. Fiber alignment in 3D collagen networks as a biophysical marker for cell contractility. Matrix Biology doi: 10.1016/j.matbio.2023.11.004 (2023). (A01, B09, C02, C05)

Chayanun S, Soufivand AA, Faber J, Budday S, Lohwongwatana B, Boccaccini AR. Reinforcing Tissue-Engineered Cartilage: Nanofibrillated Cellulose Enhances Mechanical Properties of Alginate Dialdehyde–Gelatin Hydrogel. Advanced Engineering Materials (2023) doi: 10.1002/adem.202300641. (B09)

Czerwinski T, Bischof L, Böhringer D, Kara S, Wittmann E, Winterl A, Gerum R, Nusser G, Wiesinger M, Budday S, Lux A, Voskens C, Fabry B, Mark C, doi:10.1101/2023.04.20.537658  (2023). (A01, B09, C02)

Genc H, Cianciosi A, Lohse R, Stahlhut P, Groll J, Alexiou C, Cicha I, Jungst T, Adjusting Degree of Modification and Composition of gelAGE-Based Hydrogels Improves Long-Term Survival and Function of Primary Human Fibroblasts and Endothelial Cells in 3D Cultures, Biomacromolecules doi:10.1021/acs.biomac.2c01536  (2023). (B02, B09)

Soufivand AA, Budday S. Predicting the hyperelastic properties of alginate-gelatin hydrogels and 3D bioprinted mesostructures. Scientific Reports, doi: 10.1038/s41598-023-48711-3 (2023). (B09)

Published Preparatory Work (Project B09 related)

Grossbacher G, Bartolf-Kopp M, Gergely C, Bernal PN, Florczak S, de Ruijter M, Rodriguez NG, Groll J, Malda J, Jungst T, Levato R, Volumetric Printing across Melt Electrowritten Scaffolds Fabricates Multi-Material Living Constructs with Tunable Architecture and Mechanics, Adv Mater doi:10.1002/adma.202300756  (2023) e2300756. (Z01)

2022

Janzen D, Bakirci E, Faber J, Mier MA, Hauptstein J, Pal A, Forster L, Hazur J, Boccaccini AR, Detsch R, Tessmar J, Budday S, Blunk T, Dalton PD, Villmann C, Reinforced Hyaluronic acid-based Matrices promote 3D Neuronal Network Formation, Adv Healthc Mater doi:10.1002/adhm.202201826  (2022) e2201826. (A01, A02, B06, B09, B01/C05)

Mehnert M, Faber J, Hossain M, Chester SA, Steinmann P, Experimental and numerical investigation of the electro-mechanical response of particle filled elastomers – Part I: Experimental investigations, European Journal of Mechanics – A/Solids 96 (2022). (B09)

Mehnert M, Faber J, Hossain M, Chester SA, Steinmann P, Experimental and numerical investigations of the electro-mechanical response of particle filled elastomers—Part II: Continuum modeling approach, European Journal of Mechanics – A/Solids 96 (2022). (B09)

Faber J, Hinrichsen J, Greiner A, Reiter N, Budday S, Tissue-Scale Biomechanical Testing of Brain Tissue for the Calibration of Nonlinear Material Models, Curr Protoc 2(4) (2022) e381. (B09)

Published Preparatory Work (Project B09 related)

Pien N, Bartolf‐Kopp M, Parmentier L, Delaey J, De Vos L, Mantovani D, Van Vlierberghe S, Dubruel P, Jungst T, Melt Electrowriting of a Photo‐Crosslinkable Poly( ε ‐Caprolactone)‐Based Material into Tubular Constructs with Predefined Architecture and Tunable Mechanical Properties, Macromolecular Materials and Engineering doi:10.1002/mame.202200097  (2022). (Z01) Publication image has been selected as the journal cover of the July 2022 issue of Macro Molecular Materials and Engineering

Fischhaber N, Faber J, Bakirci E, Dalton PD, Budday S, Villmann C, Schaefer N, Spinal Cord Neuronal Network Formation in a 3D Printed Reinforced Matrix-A Model System to Study Disease Mechanisms, Adv Healthc Mater doi:10.1002/adhm.202100830  (2021) e2100830. (B01, Z01)Levato R, Jungst T, Scheuring RG, Blunk T, Groll J, Malda J, From Shape to Function: The Next Step in Bioprinting, Adv Mater 32(12) (2020) 1906423. (A02, B04, Z01)

Hauptstein J, Bock T, Bartolf-Kopp M, Forster L, Stahlhut P, Nadernezhad A, Blahetek G, Zernecke-Madsen A, Detsch R, Jungst T, Groll J, Tessmar J, Blunk T, Hyaluronic Acid-Based Bioink Composition Enabling 3D Bioprinting and Improving Quality of Deposited Cartilaginous Extracellular Matrix, Adv Healthc Mater 9(15) (2020) 2000737. (A02, A06, B02, B06, Z01)

Levato R, Jungst T, Scheuring RG, Blunk T, Groll J, Malda J, From Shape to Function: The Next Step in Bioprinting, Adv Mater 32(12) (2020) 1906423. (A02, B04, Z01)

2024

Böhringer, Vogler, Bischof, Czerwinski,Cóndor, Gampl, Anh Ngo, Strissel, Strick, Budday, López-Posadas, Franze, Thievessen, Mark, Gerum, Fabry
Individual and collective force oscillations of cells and cell assemblies in 3D environments
Physics of Cancer, Leipzig, 30 September – 02 October 2024                        A01, B09,C02,C05

Jessica Faber, Jan Hinrichsen, Anahita Ahmadi Soufivand, Hsuan-Heng Lu, Aldo R. Boccaccini, and Silvia Budday
Tuning the mechanical properties of alginate dialdehyde-gelatin bioinks for bioprinting approaches by varying the oxidation degree
ELACTAM 2024 on 31st February 2024

Jessica Faber, Jan Hinrichsen, Anahita Ahmadi Soufivand, Hsuan-Heng Lu, Aldo R. Boccaccini, and Silvia Budday
Tuning the mechanical properties of alginate dialdehyde-gelatin bioinks for bioprinting approaches by varying the oxidation degree
14. Workshop für Junge Nachwuchswissenschaftler in der Mechanik on 6th March 2024

2023

Florian Hofmann
Microfluidic printheads for multi-material extrusion-based bioprinting
DGBM Jena on 15th September 2023

2022

Tomasz Jungst
Developing tissue models to study vascular diseases
Meeting of The International Society for Biofabrication 2022, Montecatini Terme (Italy) on 25-28. September 2022                      B09

Jessica Faber
Tissue-scale biomechanical testing of brain tissue
for the calibration of nonlinear material models
GAMM 2022 – 92nd GAMM Annual Meeting of the International Association of Applied Mathematics and Mechanics                            B09

2024

Böhringer, Vogler, Bischof, Czerwinski,Cóndor, Gampl, Anh Ngo, Strissel, Strick, Budday, López-Posadas, Franze, Thievessen, Mark, Gerum, Fabry
Individual and collective force oscillations of cells and cell assemblies in 3D environments
Physics of Cancer, Leipzig, 30 September – 02 October 2024                        A01, B09,C02,C05

Kirill Kriukov, Doris Schneider, Lukas Hahn, Florian Hofmann, Robert Luxenhofer, Regina Ebert (2024)
Assessment of viability and mechanoresponsiveness of hMSC-TERT printed with bioinert, thermoresponsive hydrogels
WI3R Symposium, Würzburg, 05-07 July 2024            A03, B05, B09

S.Stecher, A.Cianciosi, D.Böhringer, J.Faber, F.Dusi, P.Stahlhut, J.Teßmar, S.Budday, J.Groll, B.Fabry, P.Bauer-Kreisel and T.Blunk
Migration of Breast Cancer Cells in 3D Printable Methacrylated Collagen Type I-Based Hydrogels
WI3R Symposium, Würzburg, 5.-7.July 2024                 C02, A01, B09

S.Stecher, A.Cianciosi, D.Böhringer, J.Faber, F.Dusi1, P.Stahlhut, J.Teßmar, S.Budday, J.Groll, B.Fabry, P.Bauer-Kreisel and T.Blunk
Printable Methacrylated Collagen Type I-based Hydrogels to Study Migration of Breast Cancer Cells in a 3D Environment
7th TERMIS World Congress, Seattle, Washington, 25-28 June 2024                         C02, A01, B09

2023

Hsuan-Heng Lu, Jessica Faber, Anahita Ahmadi Soufivand, Julian Bauer, Emine Karakaya, Rainer Detsch, Oliver Friedrich, Silvia Budday, Aldo R. Boccaccini
ADA-GEL bioink system with tailored oxidation degree of ADA for biofabrication approaches
Annual Meeting of the German society for Biomaterials (DGBM) 2023 on 14th September 2023              B03, B09, B06, Z02

2022

Jessica Faber, Florian Hofmann, Silvia Budday, Tomasz Jüngst
SFB/TRR 225 Project B09: Biofabricated gradients for functional tissue models
Symposium “Biofabrication meets Infection” Würzburg on 25-28- September 2022                B09

Florian Hofmann
Microfluidic printheads for multi-material mixing NTB_058
Meeting of The International Society for Biofabrication 2022, Montecatini Terme (Italy) on 25-28. September 2022   B09

Jun-Prof. Dr. Tomasz Jüngst

• Spokesperson of the Young Scientists of the German Society for Biomaterials

Dr. Silvia Budday

• Member of the Programme Committee of the DFG’s SPP 2311
• Deputy Speaker of the Computational Biomechanics Committee of GAMM
• Women’s Representative of the Department Mechanical Engineering of FAU
• Associate Editor of the journal Computer Methods in Biomechanics and Biomedical Engineering, member of Editorial Board of the journal Scientific Reports, Guest co-editor of the journals Frontiers in Mechanical Engineering and Frontiers in Bioengineering and Biotechnology for the Special Issue “Advances in Brain Mechanics

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