B08 | Time-resolved biophotonics approach cellular signaling, cell-matrix interactions and matrix remodeling mechanisms in biofabricated constructs
In this optical engineering and bioprocess engineering project we aim to develop new systems to study in situ maturation and regulation of cell-matrix junctions of biofabricated constructs over long time periods. To this end, (i) we will advance high resolution/depth penetrating multiphoton- and light sheet technology towards a 2-photon single plane illumination microscopy (2P-SPIM), and (ii) design and engineer precision positioned mobile mini-bioreactors. We will validate these systems for selected bioconstructs within the consortium regarding (iii) visualization of 3D spatial patterning of focal adhesion proteins between cell and matrix as well as matrix production during long-term maturation of constructs.
Prof. Dr. Dr. Andreas Beilhack
Prof. Dr. Dr. Oliver Friedrich