B06 | Reporter-conjugated bioinks for the investigation of cellular interactions in biofabrication

The aim of B06 is the development of a modular reporter ink for biofabrication. The interactions between cells are to be systematically investigated in detail via integrated reporter functions in the bioink after the printing process. For this purpose, a reporter ink platform is to be developed that can be precisely printed, delivers stable 3D constructs and can be modified site-specifically in order to be able to functionally incorporate the reporters for cell adhesion and proteolytic degradation. In the long term, the reporter’s ink should enable insights into biochemical and biomechanical processes during the maturation of cells in 3D constructs.

Prof. Dr. Tessa Lühmann
Dr. Rainer Detsch

Benedikt Gantert

Previous academic education: Master Thesis
Academic Advisor within SFB/TRR 225: Prof. Dr. Tessa Lühmann
Location: University of Würzburg, Department of Pharmacy and Food chemistry
Topic of thesis: Reporter-conjugated bioinks for investigation of cellular interaction in the field of biofabrication
Main techniques and methodologies to be used: Solid Phase Peptide Synthesis, FRET-Imaging, Mass-Tag reporter with LC/MS/MS Readout, Cell culture, Bioprinting

Contact: benedikt.gantert@uni-wuerzburg.de

Lena Fischer

Previous academic education: Master Thesis
Academic Advisor within SFB/TRR 225: Dr. Ingo Thievessen
Location: Friedrich-Alexander UniversityErlangen/Nürnberg, Biophysics group, Center for Medical Physics and Technology
Topic of thesis: Cellular stress response and integrin-mediated signaling in biofabrication processes
Main techniques and methodologies to be used: Lentiviral reporter constructs, cell culture, fluorescent live cell microscopy, bioprinting

Contact: lena.lf.fischer@fau.de

Emine Karakaya

Previous academic education: B.Sc. Chemistry: “Synthesis and Characterization of the Buckminsterfullerenes C60H36und C60H18“, M. Sc. Chemistry: “Design of Goldnanorods for Nanoplasmonics”
Academic Advisor within SFB/TRR 225: Prof. Dr.-Ing. habil. Aldo R. Boccaccini, Dr. Rainer Detsch (technical advisor)
Location: Friedrich-Alexander University of Erlangen-Nuremberg, Institute of Biomaterials
Topic of thesis: Chemical functionalization of bioinks, characterization techniques, developing of bioinks for biofabrication
Main techniques and methodologies to be used: Spectroscopic methods to analyze chemical interaction of polysaccharides and peptides, bioplotting and inkjet printing, cell culture

Contact: emine.karakaya@fau.de

Karakaya E, Bider F, Frank A, Teßmar J, Schöbel L, Forster L, Schrüfer S, Schmidt H-W, Schubert DW, Blaeser A, Boccaccini AR, Detsch R, Targeted Printing of Cells: Evaluation of ADA-PEG Bioinks for Drop on Demand Approaches, Gels 8(4) (2022). (B06, A02, A04, A07)

Sivakumar S, Schmid R, Wieland A, Strissel PL, Strick R, Fischer L, Thievessen I, Kataev E, Arkudas A, Horch RE, Schubert DW, Kengelbach‐Weigand A, Role of Fiber Thickness and Surface Treatment of Electrospun Polycaprolactone Matrices on the Growth of Different Breast Cancer‐Associated Cells, Advanced Materials Interfaces doi:10.1002/admi.202101808  (2022). (B01, B06, C03)

Kappelmann-Fenzl M, Schmidt SK, Fischer S, Schmid R, Lammerhirt L, Fischer L, Schrufer S, Thievessen I, Schubert DW, Matthies A, Detsch R, Boccaccini AR, Arkudas A, Kengelbach-Weigand A, Bosserhoff AK, Molecular Changes Induced in Melanoma by Cell Culturing in 3D Alginate Hydrogels, Cancers (Basel) 13(16) (2021). (C03, A01, A07, B06)

Schmid R, Schmidt SK, Detsch R, Horder H, Blunk T, Schrüfer S, Schubert DW, Fischer L, Thievessen I, Heltmann‐Meyer S, Steiner D, Schneidereit D, Friedrich O, Grüneboom A, Amouei H, Wajant H, Horch RE, Bosserhoff AK, Arkudas A, Kengelbach‐Weigand A, A New Printable Alginate/Hyaluronic Acid/Gelatin Hydrogel Suitable for Biofabrication of In Vitro and In Vivo Metastatic Melanoma Models, Adv. Funct. Mater. doi:10.1002/adfm.202107993 (2021). (C03, A01, A07, B06, B08, C02, C04)

Sonnleitner D, Schäfer N, Wieland A, Fischer L, Pasberg P, Thievessen I, Lang G, PCL micro-dumbbells – A new class of polymeric particles reveals morphological biofunctionality, Applied Materials Today 24 (2021). (A07, B06)

Hahn L, Karakaya E, Zorn T, Sochor B, Maier M, Stahlhut P, Forster S, Fischer K, Seiffert S, Poppler AC, Detsch R, Luxenhofer R, An Inverse Thermogelling Bioink Based on an ABA-Type Poly(2-oxazoline) Amphiphile, Biomacromolecules doi:10.1021/acs.biomac.1c00427  (2021). (A03, B06)

Bauer A, Prechova M, Fischer L, Thievessen I, Gregor M, Fabry B, pyTFM: A tool for traction force and monolayer stress microscopy, PLoS Comput Biol 17(6) (2021) e1008364. (A01, B06)

Sonnleitner D, Schäfer N, Wieland A, Fischer L, Pasberg P, Thievessen I, Lang G, PCL micro-dumbbells – A new class of polymeric particles reveals morphological biofunctionality, Applied Materials Today 24 (2021). (A07, B06)

Hahn L, Karakaya E, Zorn T, Sochor B, Maier M, Stahlhut P, Forster S, Fischer K, Seiffert S, Poppler AC, Detsch R, Luxenhofer R, An Inverse Thermogelling Bioink Based on an ABA-Type Poly(2-oxazoline) Amphiphile, Biomacromolecules doi:10.1021/acs.biomac.1c00427  (2021). (A03, B06)

Genc H, Hazur J, Karakaya E, Dietel B, Bider F, Groll J, Alexiou C, Boccaccini AR, Detsch R, Cicha I, Differential Responses to Bioink-Induced Oxidative Stress in Endothelial Cells and Fibroblasts, Int. J. Mol. Sci. 22(5) (2021) 2358. (B02, A01, B06)

Distler T, Kretzschmar L, Schneidereit D, Girardo S, Goswami R, Friedrich O, Detsch R, Guck J, Boccaccini AR, Budday S, Mechanical properties of cell- and microgel bead-laden oxidized alginate-gelatin hydrogels, Biomater. Sci. 9(8) (2021) 3051-3068. (B06, B08)

Bednarzig V, Karakaya E, Egana AL, Teßmar J, Boccaccini AR, Detsch R, Advanced ADA-GEL bioink for bioprinted artificial cancer models, Bioprinting doi:10.1016/j.bprint.2021.e00145  (2021) 00145. (B06, A01, A02)

Bauer A, Prechova M, Fischer L, Thievessen I, Gregor M, Fabry B, pyTFM: A tool for traction force and monolayer stress microscopy, PLoS Comput Biol 17(6) (2021) e1008364. (A01, B06)

Mirzaei Z, Kuth S, Schubert DW, Detsch R, Roether JA, Blunk T, Kordestani SS, Boccaccini AR, Preparation and Characterization of Electrospun Blend Fibrous Polyethylene Oxide:Polycaprolactone Scaffolds to Promote Cartilage Regeneration, Adv. Eng. Mater. 22(9) (2020). (A01, A02, A07, B06)

Karakaya E, Fischer L, Hazur J, Boccaccini AR, Thievessen I, Detsch R, Strategies to evaluate alginate based bioinks applying extrusion printingfor biofabrication, Additive Manufacturing Meets Medicine, 2020. (A01, B06)

Hazur J, Detsch R, Karakaya E, Kaschta J, Tessmar J, Schneidereit D, Friedrich O, Schubert DW, Boccaccini AR, Improving alginate printability for biofabrication: establishment of a universal and homogeneous pre-crosslinking technique, Biofabrication 12(4) (2020) 045004. (A01, A02, A07, B06, B08)

Hauptstein J, Bock T, Bartolf-Kopp M, Forster L, Stahlhut P, Nadernezhad A, Blahetek G, Zernecke-Madsen A, Detsch R, Jungst T, Groll J, Tessmar J, Blunk T, Hyaluronic Acid-Based Bioink Composition Enabling 3D Bioprinting and Improving Quality of Deposited Cartilaginous Extracellular Matrix, Adv Healthc Mater 9(15) (2020) 2000737. (A02, A06, B02, B06, Z01)

Distler T, Solisito AA, Schneidereit D, Friedrich O, Detsch R, Boccaccini AR, 3D printed oxidized alginate-gelatin bioink provides guidance for C2C12 muscle precursor cell orientation and differentiation via shear stress during bioprinting, Biofabrication 12(4) (2020) 045005. (A01, B06, B08)

Distler T, Ruther F, Boccaccini AR, Detsch R, Development of 3D Biofabricated Cell Laden Hydrogel Vessels and a Low-Cost Desktop Printed Perfusion Chamber for In Vitro Vessel Maturation, Macromol. Biosci. 19(9) (2019) 1900245. (B06, A01, B03)

Steiner D, Lingens L, Fischer L, Kohn K, Detsch R, Boccaccini AR, Fey T, Greil P, Weis C, Beier JP, Horch RE, Arkudas A, Encapsulation of Mesenchymal Stem Cells Improves Vascularization of Alginate-Based Scaffolds, Tissue Eng Part A 24(17-18) (2018) 1320-1331. (C03, C04, A01, B06, B03) Front Cover for Tissue Engineering Part A

Ruther F, Distler T, Boccaccini AR, Detsch R, Biofabrication of vessel-like structures with alginate di-aldehyde-gelatin (ADA-GEL) bioink, J. Mater. Sci.: Mater. Med. 30(1) (2018) 8. (B06, A01)

Hahn L, Beudert M, Gutmann M, Keßler L, Stahlhut P, Fischer L, Lorson T, Thievessen I, Lühmann T,  Luxenhofer R (2021) Biomechanical and Biological Performances of Diels-Alder Crosslinked Thermogelling Bioink, ChemRxiv. Preprint doi:10.26434/chemrxiv.14184713.v1.

Genc H, Hazur J, Karakaya E, Dietel B, Bider F, Groll J, Alexiou C, Boccaccini AR, Detsch R, Cicha I (2021) Alginate di-Aldehyde-Induced Oxidative Stress in Endothelial Cells and Fibroblasts: Cell-specific Responses, 31st Annual Conference of the European Society for Biomaterials (ESB 2021), 5 to 9 September 2021 (A01, B02, B06)

Schmid R, Schmidt SK, Detsch R, Horder H, Blunk T, Schrüfer S, Schubert DW, Fischer L, Thievessen I, Heltmann-Meyer S, Steiner D, Schneidereit D, Friedrich O, Grüneboom A, Amouei H, Wajant H, Horch RE, Bosserhoff AK, Arkudas A, Kengelbach-Weigand A (2021) A new printable alginate / hyaluronic acid / gelatin hydrogel suitable for biofabrication of in vitro and in vivo metastatic melanoma models, 31st Annual Conference of the European Society for Biomaterials (ESB 2021), 5 to 9 September 2021 (C03, A01, A07, B06, B08, C02, C04)

Sonnleitner D, Lang G, Pasberg P, Schäfer N, Fischer L, Thievessen I (2021) PCL Micro-Dumbbells – A New Class of Polymeric Particles Reveals Morphological Biofunctionality, 31st Annual Conference of the European Society for Biomaterials (ESB 2021), 5 to 9 September 2021 (A07, B06)

Detsch R, Karakaya E, Schöbel L, Forster L, Schrüfer D, Schubert DW, Blaeser A, Teßmar J, Boccaccini AR (2021) 3D Bioprinting of ADA-PEG konjungated bioinks via drop on demand technology: Processing, mechanics and cell response. Annual Conference of the German Society for Biomaterials (DGBM); October 7 to 8, 2021 (Stuttgart, Germany)

Detsch R (2021) Engineering of living tissue exploiting innovative biomaterials and processes. Third edition of the Σ-Tech Days autumn school. 5 to 7 October 2021 (Limoges, France)

Detsch R, Karakaya E, Schöbel L, Forster L, Schrüfer D, Schubert DW, Blaeser A, Teßmar J, Boccaccini AR (2021) 3D Bioprinting of ADA-PEG konjungated bioinks via drop on demand technology: Processing, mechanics and cell response. Annual Conference of the German Society for Biomaterials (DGBM); October 7 to 8, 2021 (Stuttgart, Germany)

Detsch R (2021) Engineering of living tissue exploiting innovative biomaterials and processes. Third edition of the Σ-Tech Days autumn school. 5 to 7 October 2021 (Limoges, France)

Karakaya E, Bider F, Frank A, Schrüfer S, Boccaccini AR, Schmidt HW, Schubert D, Detsch R (2020) Comparative studies of two different sized inkjet nozzles for several bioinks based on alginate, Materials Science and Engineering (MSE), 22 – 25 September 2020 – Web conference

Hauptstein J, Böck T, Bartolf-Kopp M, Forster L, Stahlhut P, Nadernezhad A, Blahetek G, Zernecke-Madsen A, Detsch R, Jüngst T, Groll J, Teßmar J, Blunk T (2020) Hyaluronic Acid-based Bioink Composition Enabling 3D Bioprinting and Improving Quality of Deposited Cartilaginous Extracellular Matrix, Materials Science and Engineering (MSE), 22 – 25 September 2020 – Web conference

Karakya E, Fischer L, Hazur J, Boccaccini ARB, Thievessen I, Detsch R (2020) Strategies to evaluate alginate based bioinks applyingextrusion printing for biofabrication, AMMM – Additive Manufacturing Meets Medicine 2020, 9 – 11 September 2020 – Web conference

Detsch R (2020) Challenges and Limits of Bioprinting to Generate Functional Tissue Models 02.07.2020, Virtuelle MedtecLIVE 2020  MedtecLIVE 2020 & MedTech Summit GO VIRTUAL! 30. Juni bis 02. Juli 2020 https://www.medteclive.com/de/aussteller-programm

Hauptstein J, Bartolf-Kopp M, Forster L, Detsch R, Jüngst T, Groll J, Teßmar J, Blunk T (2020) Hyaluronic Acid-based Bioink Composition Enabling 3D Bioprinting and ImprovingQuality of Deposited Cartilaginous Extracellular Matrix, 5th Bioinspired Materials 2020 Conference, 16 – 19 March 2020 – Web conference

Detsch R (2019) Leben aus dem 3D Drucker: Chancen, Herausforderungen und Limitationen für verschiedene medizintechnische Anwendungen, Collegium Alexandrinum der Universität Erlangen-Nürnberg – Wissenschaft für die Öffentlichkeit – November, 28, 2019 (Erlangen Germany)

Detsch R, Hazur J, Karakaya E, Kuth S, Heid S, Boccaccini AR (2019) Evaluating three different extrusion-based bioprinting systems using cell-laden alginate-based bioinks, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)

Detsch R (2019) From the Fundamentals of Biofabrication towards Functional Tissue Models, 1st Heidelberg Workshop on 3D-Bioprinting, 18 July 2019 (Heidelberg, Germany).

Detsch R, Boccaccini AR (2019) Challenges in biofabrication of alginate-based bioinks for different biomedical approaches, Deutsches Zentrum für Luft-und Raumfahrt in Bonn, Workshop Additive Fertigung in der Raumfahrt. 28 May 2019 (Bonn, Germany)

Detsch R, Distler T, Ruther F, Boccaccini AR (2018) Engineering of Vessel Grafts using Alginate di-aldehyde – Gelatin Bioink, International Conference on Biofabrication (ISBF) 2018, 28-31 October 2018 (Würzburg, Germany)

Detsch R, Liverani L, Boccaccini AR (2018) Biomaterials research in Erlangen: from bioactive materials to tissue scaffolds and biofabrication, UMN-FAU Workshop, 19 – 20 September 2018 (University Minnesota, USA)

Karakaya E, Frank A, Forster L, Teßmar J, Schmidt HW, Boccaccini AR, Detsch R (2020) Evaluation of inkjet printing for ADA-PEG bioinks, 5th Bioinspired Materials 2020 Conference, 16 – 19 March 2020 – Web conference

Fischer L, Karakaya E, Müller S, Schrüfer S, Distler T, Schubert DW, Gekle S, Fabry B, Boccaccini AR, Detsch R, Thievessen I (2019) Shear stress and cellular behaviour in 3 D biofabrication assays, Goodbye Flat Biology: Advancing 3D-based Models for Cancer Biology and Drug Discovery – 10.-13.11.2019 (Berlin, Germany)

Karakaya E, Hazur J, Teßmar J, Boccaccini AR, Detsch R(2019) How to Determine a Suitable Sodium Alginate for the Fabrication of Standardized Alginatedialdehyde-Gelatin crosslinked Hydrogels in Biofabrication Approaches? Annual Conference of the International Society for Biofabrication (ISBF), 20-22 October 2019 (Columbus, Ohio, USA)

Karakaya E, Teßmar J, Boccaccini AR, Detsch R (2019) Evaluation of the Biopolymer Modification for the Reproducible Production of Alginate-based Bioinks, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)

Fischer L, Karakaya E, Distler T, Boccaccini AR, Fabry B, Detsch R, Thievessen I (2018) Concept studies of the application of reporter cells in biofabrication approaches, Annual Conference of the International Society for Biofabrication (ISBF), 28-31 October 2018 (Würzburg, Germany)

Fischer L, Karakaya E, Distler T, Grummel N, Boccaccini AR, Fabry B, Detsch R, Thievessen I (2018) Shear stress and cellular behaviour in 3 D biofabrication assays, 9th Annual Symposium “Physics of Cancer” 24-26 September 2018 (Leipzig, Germany)

Detsch R, Boccaccini AR (2018) Advances in Biomaterials for Biofabrication: 3D Printing with Bioinks, “Tag der Technischen Fakultät” of the FAU Erlangen 16 November 2018 (Erlangen, Germany)

Schneidereit D, Sulistio AA, Bröllochs A, Lu K, Distler T, Schürmann S, Detsch R, Dendorfer A, Boccaccini AR, Friedrich O (2018) 3D Tissue Substructure: Using Multi-Photon Excitation to Reveal Cellular 3D Morphology of Natural and Engineered Tissue in Depth, Annual Conference of the International Society for Biofabrication (ISBF), 28-31 October 2018 (Würzburg, Germany)