B05 | Membrane-Engineering as a tool to control the behavior of mesenchymal stem cells in biofabrication processes

The interaction of cells with bioinks, as well as direct cell-cell interaction, is essential for biofabrication of tissue constructs. This project aims to reduce shear stress during bioprinting, to increase cell proliferation in the hydrogel, to influence cell-cell aggregation and spheroid formation, and to optimize cell-ink interaction by coupling non-specific protective macromolecules together with specific galectin-1 ligands via cell surface modification by membrane engineering. The long-term goal is to apply membrane engineering for cell and bioink modification to optimize the post-fabrication behavior of cells in biofabricated constructs.

Prof. Dr. Regina Ebert
Prof. Dr. Jürgen Seibel

Prof. Dr. Regina Ebert

University of Wuerzburg, Orthopedic Center for Musculoskeletal Research
Contact: regina.ebert@uni-wuerzburg.de

Scientific Leadership: Boards, Committees

Prof. Dr. Jürgen Seibel

University of Wuerzburg, Institute of Organic Chemistry
Contact: seibel@chemie.uni-wuerzburg.de

Scientific Leadership: Boards, Committees

Prof. Dr. Lutz Nuhn

University of Wuerzburg, Chair of Macromolecular Chemistry
Contact:  lutz.nuhn@uni-wuerzburg.de

Scientific Leadership: Boards, Committees

Kirill Kriukov

Previous academic education: M. Sc. Functional Materials
Academic Advisor at SFB/TRR 225: Prof. Dr. Regina Ebert
Location: University of Wuerzburg, Orthopedic Center for Musculoskeletal Research
Topic of thesis: Membrane-engineering as a tool to control the behavior of mesenchymal stem cells in biofabrication processes
Main techniques and methodologies to be used: Cell culture techniques, Click chemistry (bioconjugation), Biochemical assays (proliferation, apoptosis), qPCR (differentiation, senescence, mechanotransduction), Microscopy (differentiation, senescence, viability, stiffness)

Contact: kirill.kriukov@uni-wuerzburg.de

 

 

Marcel Groß

Previous academic education:  Master-Thesis
Academic Advisor within SFB/TRR 225: Prof. Dr. Jürgen Seibel
Location: University of Wuerzburg, Institute of Organic Chemistry
Topic of thesis: Synthesis of unnatural functionalized Monosaccharids for N-Glycosylation
Main techniques and methodologies to be used: Organic Synthesis, Solid Phase Synthesis, Chromatography, Spectroscopy, Mass Spectrometry

Contact: marcel.gross@uni-wuerzburg.de

Jolana Both

Previous academic education: Master Thesis
Academic Advisor within SFB/TRR 225: Prof. Dr. Lutz Nuhn
Location: University of Wuerzburg, Chair of Macromolecular Chemistry
Topic of thesis: Repolarization of tumor-associated macrophages via mannosylated polymers and resulting nanoparticles
Main techniques and methodologies to be used: Polymer Synthesis, Organic Synthesis, Size Exclusion Chromatography, NMR-Spectroscopy

Contact: jolana.both@uni-wuerzburg.de

  • Stephan Altmann 1_sw
  • Stephan Altmann 3_sw
  • Stephan Altmann 2_sw
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  • 20230630_135248_sw_small

2023

Kockelmann J, Zentel R, Nuhn L, Post‐Polymerization Modifications to Prepare Biomedical Nanocarriers with Varying Internal Structures, their Properties and Impact on Protein Corona Formation, Macromolecular Chemistry and Physics doi:10.1002/macp.202300199  (2023). (B05)

Mut J, Altmann S, Reising S, Meissner-Weigl J, Driessen MD, Ebert R, Seibel J, SiaNAl can be efficiently incorporated in glycoproteins of human mesenchymal stromal cells by metabolic glycoengineering, ACS Biomater Sci Eng doi:10.1021/acsbiomaterials.2c01534  (2023). (B05)

Sternstein C, Bohm TM, Fink J, Meyr J, Ludemann M, Krug M, Kriukov K, Gurdap CO, Sezgin E, Ebert R, Seibel J, Development of an Effective Functional Lipid Anchor for Membranes (FLAME) for the Bioorthogonal Modification of the Lipid Bilayer of Mesenchymal Stromal Cells, Bioconjug Chem doi:10.1021/acs.bioconjchem.3c00091  (2023). (B05)

2021

Pinzner F, Keller T, Mut J, Bechold J, Seibel J, Groll J, Polyoxazolines with a Vicinally Double-Bioactivated Terminus for Biomacromolecular Affinity Assessment, Sensors 21(9) (2021). (A06, B05)

Altmann S, Mut J, Wolf N, Meissner-Weigl J, Rudert M, Jakob F, Gutmann M, Luhmann T, Seibel J, Ebert R, Metabolic Glycoengineering in hMSC-TERT as a Model for Skeletal Precursors by Using Modified Azide/Alkyne Monosaccharides, Int. J. Mol. Sci. 22(6) (2021) 2820. (B05, A03)

2020

Muller-Deubert S, Ege C, Krug M, Meissner-Weigl J, Rudert M, Bischof O, Jakob F, Ebert R, Phosphodiesterase 10A Is a Mediator of Osteogenic Differentiation and Mechanotransduction in Bone Marrow-Derived Mesenchymal Stromal Cells, Stem Cells Int. 2020 (2020) 7865484. (B05)

Herrmann M, Engelke K, Ebert R, Muller-Deubert S, Rudert M, Ziouti F, Jundt F, Felsenberg D, Jakob F, Interactions between Muscle and Bone-Where Physics Meets Biology, Biomolecules 10(3) (2020) 432. (B05)

2019

Ziouti F, Ebert R, Rummler M, Krug M, Muller-Deubert S, Ludemann M, Jakob F, Willie BM, Jundt F, NOTCH Signaling Is Activated through Mechanical Strain in Human Bone Marrow-Derived Mesenchymal Stromal Cells, Stem Cells Int. 2019 (2019) 5150634. (B05)

Ortiz-Soto ME, Reising S, Schlosser A, Seibel J, Structural and functional role of disulphide bonds and substrate binding residues of the human beta-galactoside alpha-2,3-sialyltransferase 1 (hST3Gal1), Sci. Rep. 9(1) (2019) 17993. (B05)

Gutmann M, Bechold J, Seibel J, Meinel L, Luhmann T, Metabolic Glycoengineering of Cell-Derived Matrices and Cell Surfaces: A Combination of Key Principles and Step-by-Step Procedures, ACS Biomater Sci Eng 5(1) (2019) 215-233. (A03, B05)

Ertl J, Ortiz-Soto ME, Le TA, Bechold J, Shan J, Tessmar J, Engels B, Seibel J, Tuning the Product Spectrum of a Glycoside Hydrolase Enzyme by a Combination of Site-Directed Mutagenesis and Tyrosine-Specific Chemical Modification, Chem. – Eur. J. 25(26) (2019) 6533-6541. (B05)

2018

Ortiz-Soto ME, Ertl J, Mut J, Adelmann J, Le TA, Shan J, Tessmar J, Schlosser A, Engels B, Seibel J, Product-oriented chemical surface modification of a levansucrase (SacB) via an ene-type reaction, Chem. Sci. 9(24) (2018) 5312-5321. (B05) Front Cover Picture for Chemical Science   Press Release

Gutmann M, Braun A, Seibel J, Luhmann T, Bioorthogonal Modification of Cell Derived Matrices by Metabolic Glycoengineering, ACS Biomater Sci Eng 4(4) (2018) 1300-1306. (A03, B05)

2022

Regina Ebert      Bone marrow mesenchymal stromal cells: good or bad guys?     ACHILLES Retreat Würzburg, 25.03.2022                          B05

2019

Altmann S, Mut J, Wolf N, Bechold J, Jakob F, Seibel J, Ebert R (2019) Glycoengineering as a tool to control the behavior of bone marrow-derived mesenchymal stromal cells in biofabrication processes, 1st MuSkITYR Symposium 2. – 3.12.2019 (Günzburg, Germany)

Seibel J (2019) Bioorthogonal conjugation reactions for studying biological events, Gordon Research Conference Carbohydrates, Carbohydrate Synthesis and Research to Advance Glycobiology and Biomedicine June 23 – 28, 2019 (Hong Kong, China)

2018

Seibel, J (2018) Glycoengineering as a tool for visualizing and redesigning the human and bacterial cell surfaces, GlykoT 2018, The 11th International Symposium on Glycosyltransferases, 19-23 June 2018 (Qingdao, China)

2022

Marcel Groß    SFB/TRR 225 Project B05: Membrane engineering as a tool to control the behaviour of mesenchymal stem cells in biofabrication processes    Meeting of The International Society for Biofabrication 2022, Montecatini Terme (Italy) on 25-28- September 2022 https://www.biofabrication2022.org/ B05

Kirilo Krjukov, Marcel Groß, Stephan Altmann, Denitsa Docheva, Jürgen Mut, Jürgen Seibel, Regina Ebert            Glycoengineering as a tool to control the behavior of mesenchymal stromal cells in biofabrication process                Achilles conference, 25-27.05.2022         B05

Kirilo Krjukov, Stephan Altmann, Marcel Groß, Jürgen Mut, Jürgen Seibel, Regina Ebert Membrane-Engineering as a Tool to Control the Behavior of Mesenchymal Stem Cells in Biofabrication Processes     Meeting of The International Society for Biofabrication 2022, Montecatini Terme (Italy) on 25-28- September 2022 https://www.biofabrication2022.org/   B05

Kirilo Krjukov, Stephan Altmann, Marcel Groß, Jürgen Mut, Jürgen Seibel, Regina Ebert Membrane-Engineering as a Tool to Control the Behavior of Mesenchymal Stem Cells in Biofabrication Processes     Symposium “Biofabrication meets Infection” Würzburg on 25-28- September 2022  B05

Kirilo Krjukov, Stephan Altmann, Marcel Groß, Jürgen Mut, Jürgen Seibel, Regina Ebert Glycoengineering as a Tool to Control the Behavior of Mesenchymal Stem Cells in Biofabrication Processes     Final Achilles Conference, Guimaraes, Portugal 25.-27.05.22     B05

2019

Altmann S, Mut J, Wolf N, Bechold J, Jakob F, Seibel J, Ebert R (2019) Glycoengineering as a tool to control the behavior of mesenchymal stem cells in biofabrication processes, 22nd European Congress on Alternatives to Animal Testing, 10-13 October 2019 (Linz, Austria)

Altmann S, Mut J, Wolf N, Bechold J, Jakob F, Seibel J, Ebert R (2019) Glycoengineering as a tool to control the behavior of mesenchymal stem cells in biofabrication processes, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); 9-13 September 2019 (Dresden, Germany)

Altmann S, Mut J, Wolf N, Bechold J, Jakob F, Seibel J, Ebert R (2019) Glycoengineering as a tool to control the behavior of mesenchymal stem cells in biofabrication processes, 46th European Calcified Tissue Society Congress, 11-14 May 2019 (Budapest, Hungary)

Stephan Altmann

Previous academic education: M.Sc. Biochemistry, Topic of thesis: “To be or not to be planktonic? Investigation of single species cyanobacterial biofilms”
Academic Advisor at SFB/TRR 225: PD Dr. Regina Ebert
Location: University of Wuerzburg, Orthopedic Center for Musculoskeletal Research
Topic of thesis: Glycoengineering as a tool for regulating behaviour of mesenchymal stem cells in biofabrication
Main techniques and methodologies to be used: Cell culture techniques, Click chemistry (bioconjugation), Biochemical assays (proliferation, apoptosis), qPCR (differentiation, senescence, mechanotransduction), Microscopy (differentiation, senescence, viability, stiffness)

Contact: stephan.altmann@klh.de

Jürgen Mut

Previous academic education:  Master of Science
Academic Advisor within SFB/TRR 225: Prof. Dr. Jürgen Seibel
Location: University of Wuerzburg, Institute of Organic Chemistry
Topic of thesis: Synthese von Fluor-substituierten, aromatischen Galektin-1 Inhibitoren
Main techniques and methodologies to be used: Organic synthesis

Contact: juergen.mut@uni-wuerzburg.de

Julian Bechold

Previous academic education: Master-Thesis
Academic Advisor within SFB/TRR 225: Prof. Dr. Jürgen Seibel
Location: University of Würzburg, Institute of Organic Chemistry
Topic of thesis: Synthesis of Galectin-1-Inhibitors
Main techniques and methodologies to be used: Organic Synthesis, Chromatography, Spectrometry, protein expression

Contact: julian.bechold@uni-wuerzburg.de

Natalia Wolf

Previous academic education:  M. Sc. Chemistry
Academic Advisor within SFB/TRR 225: Prof. Dr. Jürgen Seibel
Location: University of Wuerzburg, Institute of Organic Chemistry
Topic of thesis: Synthesis and application of cyanine based dyes in super resolution microscopy.
Main techniques and methodologies to be used: Organic synthesis, spectroscopy, modification and functionalization of various materials (glas slides, gold chips).

Contact: natalia.wolf@uni-wuerzburg.de

Kirilo Krjukov and Regina Ebert @AchillesH2020 Conference in Guimarães! “#Glycoengineering as a tool to control the behavior of mesenchymal stromal cells in #biofabrication process” https://achilles.i3bs.eu/

Prof. Dr. Regina Ebert

• Member of European Calcified Tissue Society (ECTS)
• Member of American Society for Bone and Mineral Research (ASMBR)

Prof. Dr. Jürgen Seibel

• Spokesperson of RTG 2581 “Metabolism, topology and compartmentation of membrane-related lipid and signalling components in infection”.
• Member of the Centre for Infection Research (ZINF)
• Editor Board, Scientific Reports, Springer Nature Publishing AG
• Editor Journal of Biosciences: Zeitschrift für Naturforschung C (ZNC), De Gruyter (Berlin, Germany)

Prof. Dr. Lutz Nuhn