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B04 | 3D printing of vascular structures from vascular wall-resident stem cells

Aim of this project is the biofabrication of artificial vascular structures with correct hierarchical organization and wall morphology consisting of intima, media and adventitia. This shall be reached by the exploitation of the cellular plasticity of vascular wall-resident stem cells (VW-SCs). These cells have been shown to possess the necessary cellular plasticity to deliver such structures. By printing of VW-SC loaded bioinks into self-healing support gels, the use of vessel wall specific matrix components and perfusion of the structures, we aim on a medium to long term to biofabricate complex and hierarchically organized vascular structures.

Prof. Dr. Süleyman Ergün
Prof. Dr. Jürgen Groll

 

Jürgen Groll

Nathaly Chicaiza-Cabezas

Previous academic education: Eng. Biotechnological Processes, MSc. Molecular Bioengineering
Academic Advisor within SFB/TRR 225: Prof. Dr. Jürgen Groll
Location: University Hospital Würzburg, Abteilung für Funktionswerkstoffe der Medizin und der Zahlheilkunde
Topic of thesis: Development of bioink formulations and printing strategies for vasculogenesis-inspired bioprinting of vasculature
Main techniques and methodologies to be used: Coaxial printing, void-free bioprinting, in-gel printing, volumetric printing, formulation of bioinks

Contact: nathaly.chicaiza@fmz.uni-wuerzburg.de

Dr. Leyla Doğan

Previous academic education:
Ege University, İzmir, TR; Post Doc. Faculty of Science, Department of Biochemistry, Project Topic: Enhancement of Gene Transfection Efficiency by Functionalization of Quantum Dot-Plasmid DNA Conjugate with YopM Protein
Izmir Institute of Technology, İzmir, TR; PhD. Faculty of Science, Department of Chemistry, Thesis topic: Synthesis and Control of Exciton Dynamics in CdTe, CdTe/CdS and ZnxCd1xTe Colloidal Nanocrystals
Izmir Institute of Technology, İzmir, TR; MSc. Faculty of Science, Department of Chemistry, Thesis topic: Hydrothermal Synthesis and Characterization of Transition Metal (Mn and V) Oxides Containing Phosphates
Middle East Technical University, Ankara, TR; Bachelor of Science, Faculty of Science, Department of Chemistry
Academic Advisor within SFB/TRR 225: Prof. Süleyman Ergün
Location: University Hospital Würzburg, Institut für Anatomie und Zellbiologie
Topic: Post-Doc Researcher, project is related with 3D printing of vascular structures from vascular wall-resident cells
Main techniques and methodologies to be used: Stem cells (hiFSiPS cells, VW-RS Cells), isolation, expansion & differentiation, immunohistochemistry and evolution assays, imaging-including light, laser and electron microscopy techniques, chorio-allantoic membrane (CAM) assay, material building, fluorescence and absorption spectrophotometers, spectroscopic techniques, molecular imaging, 3D bioprinting techniques

Contact: leyla.dogan@uni-wuerzburg.de

2024

Doryab A, Braig J, Jungst T, Ryma M, Groll J. Fabrication Strategies for Bioinspired and Functional Lung-on-Chips. Adv. Funct. Mater. doi: 10.1002/adfm.20240772 (2024). (B02, B04, B09)

2023

Rockel AF, Wagner N, Spenger P, Ergun S, Worsdorfer P, Neuro-mesodermal assembloids recapitulate aspects of peripheral nervous system development in vitro, Stem Cell Reports doi:10.1016/j.stemcr.2023.03.012  (2023). (B04)

Wörsdörfer P, Ergün S, “Organoids”: Insights from the First Issues, Organoids 2(2) (2023) 79-81. (B04)

2022

Nadernezhad A, Groll J, Machine Learning Reveals a General Understanding of Printability in Formulations Based on Rheology Additives, Adv Sci (Weinh) doi:10.1002/advs.202202638  (2022) e2202638. (B04, A06)

Ergün S, Wörsdörfer P, Organoids, Assembloids and Embryoids: New Avenues for Developmental Biology, Disease Modeling, Drug Testing and Toxicity Assessment without Animal Experimentation, Organoids 1(1) (2022) 37-40. (B04)

Kleefeldt F, Upcin B, Bommel H, Schulz C, Eckner G, Allmanritter J, Bauer J, Braunger B, Rueckschloss U, Ergun S, Bone marrow-independent adventitial macrophage progenitor cells contribute to angiogenesis, Cell Death Dis 13(3) (2022) 220. (B04)

2021

Dogan L, Scheuring R, Wagner N, Ueda Y, Wörsdörfer P, Groll J, Ergün S, Human iPSC-derived mesodermal progenitor cells preserve their vasculogenesis potential after extrusion and form hierarchically organized blood vessels, Biofabrication 13(4)  (2021). (B04)

Horder H, Guaza Lasheras M, Grummel N, Nadernezhad A, Herbig J, Ergun S, Tessmar J, Groll J, Fabry B, Bauer-Kreisel P, Blunk T, Bioprinting and Differentiation of Adipose-Derived Stromal Cell Spheroids for a 3D Breast Cancer-Adipose Tissue Model, Cells 10(4) (2021) 803. (C02, A02, B02, B04)

Sun W, Starly B, Daly AC, Burdick JA, Groll J, Skeldon G, Shu W, Sakai Y, Shinohara M, Nishikawa M, Jang J, Cho DW, Nie M, Takeuchi S, Ostrovidov S, Khademhosseini A, Kamm RD, Mironov V, Moroni L, Ozbolat IT, The bioprinting roadmap, Biofabrication 12(2) (2020) 022002. (B02, B04)

2020

Nadernezhad A, Forster L, Netti F, Adler-Abramovich L, Tessmar J, Groll J, Rheological analysis of the interplay between the molecular weight and concentration of hyaluronic acid in formulations of supramolecular HA/FmocFF hybrid hydrogels, Polym. J. 52(8) (2020) 1007-1012. (A02, B04)

Liebscher J, Tessmar J, Groll J, In Situ Polymer Analogue Generation of Azlactone Functions at Poly(oxazoline)s for Peptide Conjugation, Macromol. Chem. Phys. 221(1) (2020) 1900500. (A06, B04)

Levato R, Jungst T, Scheuring RG, Blunk T, Groll J, Malda J, From Shape to Function: The Next Step in Bioprinting, Adv. Mater. 32(12) (2020) 1906423. (A02, B04, Z01)

2019

Berberich O, Blöhbaum J, Hölscher-Doht S, Meffert RH, Teßmar J, Blunk T, Groll J, Catechol-modified poly(oxazoline)s with tunable degradability facilitate cell invasion and lateral cartilage integration, J. Ind. Eng. Chem. 80 (2019) 757-769. (A06, B04)

2018

Groll J, Burdick JA, Cho DW, Derby B, Gelinsky M, Heilshorn SC, Jungst T, Malda J, Mironov VA, Nakayama K, Ovsianikov A, Sun W, Takeuchi S, Yoo JJ, Woodfield TBF, A definition of bioinks and their distinction from biomaterial inks, Biofabrication 11(1) (2018) 013001. (A06, B02, B04)

2023

Nathaly Chicaiza Cabezas
3D Bioprinting of vasculature based on induced vasculogenesis
DGBM Jena on 15th September 2023

2022

Ruben Gerrit Scheuring
Mind the Process – How to Boost Reproducibility, Precision & Printing Time of Gelatin-based Bioinks B&B_062
Meeting of The International Society for Biofabrication 2022, Montecatini Terme (Italy) on 25-28. September 2022                          B04

2021

Horder H, Guaza Lasheras M, Grummel N, Nadernezhad A, Herbig J, Ergün S, Teßmar J, Groll J, Fabry B, Bauer-Kreisel P, Blunk T (2021)
A 3D Bioprinted Breast Cancer-Adipose Tissue Model Utilizing Adipose-Derived Stromal Cell Spheroids
31st Annual Conference of the European Society for Biomaterials (ESB 2021), 5 to 9 September 2021 (A02, B02, B04, C02)

2019

Groll J (2019)
3D Printable Hydrogel-Bioinks: Rheological Implications and the Importance of Controlled Network Formation
TERMIS European Chapter Meeting 2019 27th-31st May 2019, Rhodes, Greece Tissue Engineering Therapies: From Concept to Clinical Translation & Commercialisation

Groll J (2019)
Biofabrication – Chances, Challenges and Limits
Euro BioMAT 2019, 08 – 09 May 2019 (Jena-Weimar, Germany)

2018

Groll J (2018)
Biofabrication research at FMZ: from solution electrospun meshes to thiol-ene cross-linked hydrogel-bioinks
International Conference on Additive Manufacturing and Biomanufacturing,  2-4 December 2018 (Beijing, China)

Groll J (2018)
Bio-ink development for Biofabrication
International Conference on Emerging Healthcare Materials, 15-16 November 2018 (Seoul, South-Korea)

Groll J (2018)
SFB/TRR 225: From the Fundamentals of Biofabrication to Functional Tissue Models
International Conference on Biofabrication (ISBF) 2018, 28-31 October 2018 (Würzburg, Germany)

Groll J (2018)
Hydrogels as Bioinks for Biofabrication
MSE conference, 26 – 28 September 2018, (Darmstadt, Germany)

Groll J (2018)
Melt Electro Writing at the Department of Functional Materials in Medicine and Dentistry
Annual Meeting of the European Society for Biomaterials,  10 – 13 September2018 (Maastricht, Netherlands)

Groll J (2018)
Biofabrication: Status of the field and translational perspective
3D-BioNET-working-event,  18 July 2018 (Freiburg im Breisgau, Germany)

Groll J (2018)
Biotinten: 3D-Druckbare Hydrogele als Schlüssel zum BioPrinting
Materialinnovationen 2018 – Vom Material zur Innovation, Konferenz zur Materialforschung des BMBF, 4. – 6. June 2018, (München, Germany)

Groll J (2018)
Hydrogels as “Bio-inks” for Biofabrication
2nd Theodore von Kármán – Discussion Conference on Materials for Life: Bioinspired and Biomimetic Hydrogels, 15 – 18 April 2018, (Bensberg, Germany).

Groll J (2018)
Bioinks: Concepts, Rheological Considerations and Recent Trends
TERMIS workshop on 3D Printing in Musculoskeletal Tissue Engineering: Bioprinting in Action, 23 March 2018 (Warsaw, Poland)

Groll J (2018)
3D-Druckbare Hydrogele als Tinten für die Biofabrikation
Thüringer Biomaterial Kolloquium, 13. – 15. March 2018 (Zeulenroda, Germany)

2023

Nathaly Chicaiza Cabezas, L. Dogan, J. Groll, S. Ergün
Vasculogenesis by hiMPCs in vascular formation using 3D bioprinting
ISBF Biofabrication 2023 on 17th September 2023

Jana Kircher, Taufiq Ahmad, Jürgen Groll, Süleyman Ergün, Philipp Wörsdörfer, Leyla E. Dogan
Large Scale production of Blood Vessel Organoids as building blocks for Biofabrication
The 117th Annual Meeting of the Anatomical Society on 28th September 2023

Leyla E. Dogan, Nathaly Chicaiza-Cabezas, Jürgen Groll, Süleyman Ergün, Philipp Wörsdörfer
Biofabrication of blood vessels from hiPSC-derived mesodermal progenitor cells
The 117th Annual Meeting of the Anatomical Society on 28th September 2023

2022

Nathaly Chicaiza Cabezas, Leyla Dogan, Ruben Scheuring1, Philipp Wörsdörfer, Jürgen Groll, Süleyman Ergün
SFB/TRR 225 Project B04: 3D Bioprinting of Vascular Structures using Vascular Wall-Resident Stem Cells and Human iPSC-derived Multipotent Progenitor Cells
Symposium “Biofabrication meets Infection” Würzburg on 25-28- September 2022       B04

Leyla Dogan
3D Bioprinting of Vascular Structures using Vascular Wall-Resident Stem Cells and Human iPSC-derived Multipotent Progenitor Cells NTB_050
Meeting of The International Society for Biofabrication 2022, Montecatini Terme (Italy) on 25-28- September 2022 https://www.biofabrication2022.org/ B04

2020

Doğan L, Scheuring RG, Groll J, Ergün S (2020)
3D Printing of Vascular Structures From Stem and Progenitor Cells
5th Bioinspired Materials 2020 Conference, 16 – 19 March 2020 – Web conference

2019

Scheuring RG, Dogan L, Groll J, Ergün S (2019)
3D Printing of vascular wall-resident stem cells into tubular structures
30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)

Ruben Gerrit Scheuring

Previous academic education: 03/2012 – 09/2015 Bachelor’s program “Molecular Biology”, Paris Lodron University Salzburg, Johannes Kepler University Linz, Austria; 09/2015 – 09/2017 Master’s program “Tissue Engineering and Regenerative  Medicine” Technikum Wien, Austria
Academic Advisor within SFB/TRR 225: Prof. Dr. Jürgen Groll
Location: University Hospital Würzburg, Abteilung für Funktionswerkstoffe der Medizin und der Zahlheilkunde
Topic of thesis: 3D Printing of Vascular Structures from Vascular Wall-Resident Stem Cells
Main techniques and methodologies to be used: Bioprinting via freeform reversible embedding, establishment of coaxial 3D bioprinting, optimization of bioinks, rheology, development of a perfusion bioreactor for tissue maturation, stem cell isolation, expansion and differentiation, cell culture, viability and functionality assays, molecular biological investigations, microscopy (light, laser, confocal and electron), in vivo tests (CAM-assay)

Contact: ruben.scheuring@fmz.uni-wuerzburg.de

Prof. Dr. Jürgen Groll

Prof. Dr. Süleyman Ergün

• Chairman of the Anatomical Society
• Member of the Board of the Anatomical Society
• Member of Editorial Board of Histochemistry and Cell Biology, Annals of Anatomy

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