B03 | Printing of biofabricate and customized bioreactors for skeletal muscle tissue

The aim of subproject B03 is the development of different composite bioinks with ion-releasing (bioreactive) particles and electrically conductive fillers for the bioprinting of skeletal muscle tissue.

Basically, synergistic stimulation of cells and cell growth through electrical and biochemical factors for the formation of highly mature muscle tissue will be investigated in vitro. For this purpose, a bioreactor with complex structure and functionalities will be designed and fabricated using additive manufacturing, which will be tissue-specific and will enable the electromechanical stimulation of skeletal muscle tissue.

Prof. Dr. Aldo R. Boccaccini
Dr. Sahar Salehi
Prof. Dr. Frank Döpper

Hsuan-Heng Lu

Previous academic education: M.Sc. in Advanced Materials and Processes (MAP)

Academic Advisor at SFB/TRR 225: Prof. Dr.-Ing. habil. Aldo R. Boccaccini
Location: Friedrich-Alexander University of Erlangen-Nuremberg, Institute of Biomaterials
Topic of thesis: Bioprinting of composite hydrogels for muscle tissue regeneration
Main techniques and methodologies to be used: Bioprinting, hydrogel processing, spectroscopy, microscopy, mechanical tests, rheology, ion release studies, cell culture

Contact: hsuan-heng.lu@fau.de

Lys Sprenger

Previous academic education: Master Thesis at Fraunhofer IFAM
Academic Advisor within SFB/TRR 225: Dr. Sahar Salehi
Location: University of Bayreuth, Department of biomaterials, Faculty of Engineering
Topic of thesis: Conductive ribbon-hydrogel scaffold engineering for skeletal muscle bio-printing (to be defined)
Main techniques and methodologies to be used: 3D-priting, cell culture assays, tensile strength, rheology, SEM, FTIR

Contact: lys.sprenger@bm.uni-bayreuth.de

Johann Schorzmann

Previous academic education: Master of Science in studies for industrial engineering at University of Bayreuth
Master thesis: „ Lightweight design with topology optimization for a endless fibre reeinforced hybrid part manufactured by additive manufacturing and injection molding“
Academic Advisor within SFB/TRR 225: Prof. Dr.-Ing Frank Döpper
Location: University of Bayreuth, Chair Manufacturing and Remanufacturing Technology
Topic of thesis: Electrical conductivity in polymer based additive manufacturing for sensor application
Main techniques and methodologies to be used: Additive Manufacturing (polymer powder based, Dispenser and Materialextrusion), Measuring devices for actor and sensor evaluation

Contact: Johann.Schorzmann@uni-bayreuth.de

Susanne Heid

Previous academic education: Bachelor of Science in Nanotechnology, Master of Science in Nanotechnology – passed with distinction: “Tissue Plasminogen Activator Functionalized Iron Oxide Nanoparticles for Targeted Fibrin Clot Dissolution”
Academic Advisor at SFB/TRR 225: Prof. Dr.-Ing. habil. Aldo R. Boccaccini, Dr. Rainer Detsch (technical advisor)
Location: Friedrich-Alexander University of Erlangen-Nuremberg, Institute of Biomaterials
Topic of thesis: Simultaneous printing of biofabricated constructs and bioreactors: composite bioinks and non-degradable polymers for regenerative approaches
Main techniques and methodologies to be used: Hard and soft printing, mechanical studies, rheology, spectroscopy, microscopy, cell culture

Contact: susanne.heid@fau.de

Poster prize at the 30`the ESB/26`the DGBM Conference in Dresden from 09`th -13`th September 2019

Bider F, Karakaya E, Mohn D, Aldo R, Boccaccini, Advantages of nanoscale bioactive glass as inorganic filler in alginate hydrogels for drug delivery and biofabrication, European Journal of Materials 2(1) (2022) 33-53. (B03)

Heid S, Becker K, Byun J, Biermann I, Neščáková Z, Zhu H, Groll J, Boccaccini AR, Bioprinting with bioactive alginate dialdehyde-gelatin (ADA-GEL) composite bioinks: Time-dependent in-situ crosslinking via addition of calcium-silicate particles tunes in vitro stability of 3D bioprinted constructs, Bioprinting doi:10.1016/j.bprint.2022.e00200  (2022). (B03, A01, A06)

Apsite I, Salehi S, Ionov L, Materials for Smart Soft Actuator Systems, Chem Rev doi:10.1021/acs.chemrev.1c00453  (2021). (B03)

Uribe-Gomez J, Posada-Murcia A, Shukla A, Ergin M, Constante G, Apsite I, Martin D, Schwarzer M, Caspari A, Synytska A, Salehi S, Ionov L, Shape-Morphing Fibrous Hydrogel/Elastomer Bilayers Fabricated by a Combination of 3D Printing and Melt Electrowriting for Muscle Tissue Regeneration, ACS Appl. Bio Mater. 4(2) (2021) 1720-1730. (B03)

Reakasame S, Dranseikiene D, Schrüfer S, Zheng K, Schubert DW, Boccaccini AR, Development of alginate dialdehyde-gelatin based bioink with methylcellulose for improving printability, Mat Sci Eng C-Mater doi:10.1016/j.msec.2021.112336  (2021) (B03, A07)

Heltmann-Meyer S, Steiner D, Muller C, Schneidereit D, Friedrich O, Salehi S, Engel FB, Arkudas A, Horch RE, Gelatin methacryloyl is a slow degrading material allowing vascularization and long-term usein vivo, Biomed Mater 16(6) (2021). (B03, B08, C01, C04)

Uribe-Gomez J, Posada-Murcia A, Shukla A, Ergin M, Constante G, Apsite I, Martin D, Schwarzer M, Caspari A, Synytska A, Salehi S, Ionov L, Shape-Morphing Fibrous Hydrogel/Elastomer Bilayers Fabricated by a Combination of 3D Printing and Melt Electrowriting for Muscle Tissue Regeneration, ACS Appl. Bio Mater. 4(2) (2021) 1720-1730. (B03)

Mueller C, Trujillo-Miranda M, Maier M, Heath DE, O’Connor AJ, Salehi S, Effects of External Stimulators on Engineered Skeletal Muscle Tissue Maturation, Adv. Mater. Interfaces 8(1) (2021) 2001167. (B03)

Hessenauer MET, Vaghela R, Korner C, von Horsten S, Pobel C, Gage D, Muller C, Salehi S, Horch RE, Arkudas A, Watching the vessels grow – establishment of intravital microscopy in the AV-loop rat model, Tissue Eng Part C Methods doi:10.1089/ten.TEC.2021.0024  (2021). (C04, B03)

Farasatkia A, Kharaziha M, Ashrafizadeh F, Salehi S, Transparent silk/gelatin methacrylate (GelMA) fibrillar film for corneal regeneration, Mater Sci Eng C Mater Biol Appl 120 (2021) 111744. (B03)

Constante G, Apsite I, Alkhamis H, Dulle M, Schwarzer M, Caspari A, Synytska A, Salehi S, Ionov L, 4D Biofabrication Using a Combination of 3D Printing and Melt-Electrowriting of Shape-Morphing Polymers, ACS Appl Mater Interfaces 13(11) (2021) 12767-12776. (B03)

Cai FF, Heid S, Boccaccini AR, Potential of Laponite(R) incorporated oxidized alginate-gelatin (ADA-GEL) composite hydrogels for extrusion-based 3D printing, J. Biomed. Mater. Res., Part B doi:10.1002/jbm.b.34771 (2021) 1 – 15. (B03)

Salehi S, Koeck K, Scheibel T, Spider Silk for Tissue Engineering Applications, Molecules 25(3) (2020) 737. (B03, C01)

Russell CS, Mostafavi A, Quint JP, Panayi AC, Baldino K, Williams TJ, Daubendiek JG, Hugo Sánchez V, Bonick Z, Trujillo-Miranda M, Shin SR, Pourquie O, Salehi S, Sinha I, Tamayol A, In Situ Printing of Adhesive Hydrogel Scaffolds for the Treatment of Skeletal Muscle Injuries, ACS Appl. Bio Mater. 3(3) (2020) 1568-1579. (B03)

Heid S, Boccaccini AR, Advancing bioinks for 3D bioprinting using reactive fillers: A review, Acta Biomater 113 (2020) 1-22. (B03)

Gensler M, Leikeim A, Mollmann M, Komma M, Heid S, Muller C, Boccaccini AR, Salehi S, Groeber-Becker F, Hansmann J, 3D printing of bioreactors in tissue engineering: A generalised approach, PLoS One 15(11) (2020) 0242615. (B03)

Apsite I, Constante G, Dulle M, Vogt L, Caspari A, Boccaccini AR, Synytska A, Salehi S, Ionov L, 4D Biofabrication of fibrous artificial nerve graft for neuron regeneration, Biofabrication 12(3) (2020) 035027. (B03)

Aigner TB, Haynl C, Salehi S, O’Connor A, Scheibel T, Nerve guidance conduit design based on self-rolling tubes, Mater Today Bio 5 (2020) 100042. (C01, B03)

Wicklein VJ, Singer BB, Scheibel T, Salehi S, Chapter 17 – Nanoengineered biomaterials for corneal regeneration, Micro and Nano Technologies (2019) pp. 379-415. (B03)

Schwedhelm I, Zdzieblo D, Appelt-Menzel A, Berger C, Schmitz T, Schuldt B, Franke A, Muller FJ, Pless O, Schwarz T, Wiedemann P, Walles H, Hansmann J, Automated real-time monitoring of human pluripotent stem cell aggregation in stirred tank reactors, Sci. Rep. 9(1) (2019) 12297. (B03)

Ostrovidov S, Salehi S, Costantini M, Suthiwanich K, Ebrahimi M, Sadeghian RB, Fujie T, Shi X, Cannata S, Gargioli C, Tamayol A, Dokmeci MR, Orive G, Swieszkowski W, Khademhosseini A, 3D Bioprinting in Skeletal Muscle Tissue Engineering, Small 15(24) (2019) 1805530. (B03)

Distler T, Ruther F, Boccaccini AR, Detsch R, Development of 3D Biofabricated Cell Laden Hydrogel Vessels and a Low-Cost Desktop Printed Perfusion Chamber for In Vitro Vessel Maturation, Macromol. Biosci. 19(9) (2019) 1900245. (B06, A01, B03)

Apsite I, Uribe JM, Posada AF, Rosenfeldt S, Salehi S, Ionov L, 4D biofabrication of skeletal muscle microtissues, Biofabrication 12(1) (2019) 015016. (B03)

Steiner D, Lingens L, Fischer L, Kohn K, Detsch R, Boccaccini AR, Fey T, Greil P, Weis C, Beier JP, Horch RE, Arkudas A, Encapsulation of Mesenchymal Stem Cells Improves Vascularization of Alginate-Based Scaffolds, Tissue Eng Part A 24(17-18) (2018) 1320-1331. (C03, C04, A01, B06, B03) Front Cover for Tissue Engineering Part A

Heid S, Becker K, Byun J, Lu HH, Boccaccini AR (2021) Advanced modification of alginate dialdehyde-gelatine composite bioinks with bioactive inorganic fillers for 3D bioprinting approaches, 31st Annual Conference of the European Society for Biomaterials (ESB 2021), 5 to 9 September 2021 (B03)

Trujillo Miranda M, Müller C, Salehi S (2020) Effect of external stimulus on biofabricated skeletal muscle microtissues, 11th World biomaterials congress WBC 2020 11 – 15 December 2020 — Web conference

Russell CS, Mostafavi A, Quint JP, Panayi A, Udeh K, Williams TJ, Daubendiek JG, Sánchez VH, Bonick Z, Trujill-Miranda M, Ryon Shin S, Pourquie O, Salehi S, Sinha I, Tamayol A (2020) In situ printing of gelatin-based scaffolds for the treatment of muscle injuries, World biomaterials congress WBC 2020 Glasgow 11 – 15 December 2020 – Web conference

Heid S, Lu HH, Venkatraman A, Detsch R, Boccaccini AR (2020) Evaluating the 3D Printaility of ADA-GEL-based Composite Bioinks, Materials Science and Engineering (MSE), 22 – 25 September 2020 – Web conference

Salehi, S (2020) Application of micro/nano scale substrates for skeletal muscle tissue regeneration, 8th International Conference on Nanoscience and Nanotechnology (ICONN 2020) 9 – 13 February 2020, Bisbane, Australia

Detsch R, Hazur J, Karakaya E, Kuth S, Heid S, Boccaccini AR (2019) Evaluating three different extrusion-based bioprinting systems using cell-laden alginate-based bioinks, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)

Trujillo Miranda M, Uribe J, Apsite I, DeSimone E, Salehi S, Ionov L (2019) Fabrication of tubular structure to mimic the osteon structure, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)

Heid S, Detsch R, Boccaccini AR (2019) New Alginate-Based Composite Bioinks for Enhanced 3D Bioprinting; TERMIS EU Workshop 2019, August, 26 to 27 2019, (Nantes, France)

Salehi S (2019) Mimicking the osteon structure by biofabrication techniques, TERMIS European Chapter Meeting 2019 27th-31st May 2019, Rhodes, Greece Tissue Engineering Therapies: From Concept to Clinical Translation & Commercialisation

Salehi S, Kharaziha M, Ionov L, Scheibel T. (2019) Composite ink development from bioceramic nanoparticles for bone tissue regeneration, 9th biennial Australian Colloid and Interface Symposium (ACIS) 3 – 7 February 2019 (Hobart, Tasmania, Australia)

Boccaccini AR (2018) Bioinks containing reactive fillers in biofabrication approaches, Invited presentation at West Pomeranian University of Technology, 22 November 2018 (Szczecin, Poland)

Boccaccini AR (2018) Bioactive Materials and Biofabrication for Biomedical Applications: Progress and Challenges, Invited speaker at Scientific Workshop 2018, Rostock University Medicine (Universitätsmedizin Rostock), 16 November 2018 (Rostock, Germany)

Boccaccini A. (2018) Bioactive materials and biofabrication for tissue engineering: achievements and challenges”, Invited presentation at Polytechnic School (Escuela Politecnica Superior, EPS), University of Seville, 31st October 2018 (Seville, Spain).

Boccaccini A. (2018) Development of composite bioinks incorporating bioreactive inorganic particles, Invited presentation at: International Conference on Biofabrication (ISBF) 2018, 28-31 October 2018 (Würzburg, Germany).

Boccaccini A. (2018) Biofabrication with alginate di-aldehyde / gelatin (ADA/GEL) hydrogels: progress, applications and challenges, Oral presentation at: Biomaterials and Novel Technologies for Healthcare, 2nd International Biennial Conference BioMaH, Frascati, 8-11 October, 2018 (Rome, Italy).

Boccaccini A. (2018) Bioactive materials and biofabrication for tissue engineering applications. Invited seminar at: CONICET Laboratory of Integration of Cellular Signals at the Institute for Histology and Embryology, Faculty of Medicine, National University of Cuyo (UNCUYO), 27th August 2018 (Mendoza, Argentina).

Boccaccini A. (2018) Bioactive materials and biofabrication for bone and soft tissue engineering, Invited lecture at: Justus-Liebig-University Giessen, Medical School, 19th July 2018 (Giessen, Germany).

Boccaccini A. (2018) Bioactive materials and biofabrication for tissue engineering: progress and challenges. Invited speaker at: Junior EUROMAT 2018 Conference, 8-12 July 2018 (Budapest, Hungary).

Boccaccini A. (2018) Enhancing alginate based bioinks for biofabrication: the composites approach, Invited speaker at: 1st TERMIS-EU Workshop on “3D Printing in Musculoskeletal Tissue Engineering”, 22-23 March 2018 (Warsaw, Poland).

Boccaccini A. (2018) Bioactive materials and biofabrication approaches for hard tissue regeneration, Invited speaker at: 2nd Sino-German Symposium on Reconstruction and Repair of Musculoskeletal Tissues, Mainz University Medical Center, 13-14 March 2018 (Mainz, Germany).

Müller C, Salehi S (2020) Fabrication of support structures for 3D bioprinting skeletal muscle cells, World biomaterials congress WBC 2020 Glasgow, 11 – 15 December 2020 – Web conference

Müller C, Gensler M, Heid S, Boccaccini AR, Detsch R, Hansmann J, Salehi S (2020) Simultaneous printing of biofabricates and tailored bioreactors, 5th Bioinspired Materials 2020 Conference, 16 – 19 March 2020 – Web conference

Karakaya E, Hazur J, Teßmar J, Boccaccini AR, Detsch R(2019) How to Determine a Suitable Sodium Alginate for the Fabrication of Standardized Alginatedialdehyde-Gelatin crosslinked Hydrogels in Biofabrication Approaches? Annual Conference of the International Society for Biofabrication (ISBF), 20-22 October 2019 (Columbus, Ohio, USA)

Heid S, Detsch R, Boccaccini AR (2019) Is There an Optimum in the Design of Alginate-Based Composite Bioinks for 3D Bioprinting Regarding the Filler Concentration? Annual Conference of the International Society for Biofabrication (ISBF), 20-22 October 2019 (Columbus, Ohio, USA)

Heid S, Detsch R, Boccaccini AR (2019) Alginate-Based Composite Bioinks for 3D Biofabrication, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)

Salehi S (2019) Electrospun Stimuli-Responsive Mats for Muscle Tissue Regeneration, TeRMIS European Chapter Meeting 2019 27th-31st May 2019, Tissue Engineering Therapies: From Concept to Clinical Translation & Commercialisation (Rhodes, Greece)

Detsch R, Boccaccini AR (2018) Advances in Biomaterials for Biofabrication: 3D Printing with Bioinks, “Tag der Technischen Fakultät” of the FAU Erlangen 16 November 2018 (Erlangen, Germany)

Salehi S, Ionov L, Scheibel T (2018) Bioactive Composite Ink for Bone Tissue Regeneration, Annual Conference of the International Society for Biofabrication (ISBF), Würzburg, Germany, on 28-31 October, 2018.

Gensler M, Komma M, Heid S, Detsch R, Salehi S, Boccaccini AR, Hansmann J (2018) Bioreactor concepts for simultaneous printing of bioreactor and tissue equivalent, Annual Conference of the International Society for Biofabrication (ISBF), 28-31 October 2018 (Würzburg, Germany)

Claudia Müller

Previous academic education: Diploma thesis
Academic Advisor within SFB/TRR 225: Dr. Sahar Salehi
Location: University of Bayreuth, Department of biomaterials, Faculty of Engineering
Topic of thesis: Simultaneous printing of biofabricated constructs and bioreactors: composite bioinks for regenerative approaches (skeletal muscle tissue)
Main techniques and methodologies to be used: Soft-printing, rheology, mechanical tests, spectroscopy, microscopy and cell culture.

Contact: Claudia1.Mueller@uni-bayreuth.de

Marius Gensler

Previous academic education: Master (Biology)
Academic Advisor within SFB/TRR 225: Dr.-Ing. Jan Hansmann
Location: University of Würzburg, Department Tissue Engineering and Regenerative Medicine
Topic of thesis: Simultaneous printing of bioreactor and tissue (working title)
Main techniques and methodologies to be used: In Silico Simulations, Cell Culture, 3D Printing, Bioreactor Handling

Contact: marius.gensler@uni-wuerzburg.de