B02 | Pre-endothelialized perfusable microvascular networks for biofabrication of standardized in vitro tissue models.

The aim of this project is biofabrication of perfusable and endothelialized microvascular networks, which will be produced by MEW of sacrificial thermoresponsive polyoxazolines. Particular focus will be placed on adapting the network geometry and the surrounding cell-containing hydrogels to tissue-specific requirements of B and C projects and to the design of appropriate microscopy-compliant bioreactors. Besides, extensive investigations of endothelial function in the fabricated tissue models are planned, including angiogenesis, interactions with circulating cells, barrier function and regulation of physiological processes in the hydrogel-contained cells.

Prof. Dr. Iwona Cicha
Prof. Dr. Jürgen Groll

Jürgen Groll

Hatice Genc

Previous academic education: Master thesis: Anti-angiogenic drug development, PhD course: Nanoparticles for Photodynamic Cancer Therapy, Research projects: Nanoparticle Biocompatibility
Academic Advisor within SFB TRR225: PD Dr. Iwona Cicha
Location: University Hospital Erlangen, Section of Experimental Oncology and Nanomedicine
Topic of thesis: Pre-endothelialized perfusable microvascular networks for biofabrication of standardized in vitro tissue models
Main techniques and methodologies to be used: Cell Colonization, Cell response to hydrogels, Biocompatibility, Functionality of microvascular networks, Angiogenesis

Contact: hatice.genc@uk-erlangen.de

Robin Dai

Previous academic education: Master thesis (Life Science Engineering)
Academic Advisor within SFB TRR225: Prof. Dr. Iwona Cicha
Location: University Hospital Erlangen, Section of Experimental Oncology and Nanomedicine
Topic of thesis: Biofabrication of endothelialized perfusable microvascular structures and ther adaptation to 3D tissue models
Main techniques and methodologies to be used: 3D cell culture, spheroids, perfusion systems & bioreactors, confocal microscopy & 3D imaging, functional cell assays,

Contact: Robin.Dai@uk-erlangen.de

Sven Heilig

Previous academic education: Master Funktionswerkstoffe
Academic Advisor within SFB TRR225: Prof. Dr. Jürgen Groll
Location: Department of functional material in medicine and dentistry, University of Würzburg
Topic of thesis: Perfusable endothelialized networks per oxaxolnine sacrificial structures
Main techniques and methodologies to be used: Cell culture, Stereolithography, Melt Electrospinning Writing

Contact: sven.heilig@fmz.uni-wuerzburg.de

Dr. Matthias Ryma

Location: Department of functional material in medicine and dentistry, University of Würzburg
Contact: matthias.ryma@fmz.uni-wuerzburg.de

Dissertation Award: “Die unterfränkische Gedenkstiftung für Wissenschaft und die Julius-Maximilians-Universtität Würzburg zeichnen Herrn Dr. rer. nat. Matthias Ryma in Anerkennung der mit dem Werk “Anwendung der Thermoresponsivität von Poly(2-oxazolin) für die Biofabrikation” geleisteten wissenschaftlich herausragenden Arbeit durch Verleihung eines Promotionspreises aus.” Würzburg, 11. Mai 2022

2022

Ryma M, Genc H, Nadernezhad A, Paulus I, Schneidereit D, Friedrich O, Andelovic K, Lyer S, Alexiou C, Cicha I, Groll J, A print-and-fuse Strategy for Sacrificial Filaments Enables Biomimetically Structured Perfusable Microvascular Networks with Functional Endothelium Inside 3D Hydrogels, Adv Mater doi:10.1002/adma.202200653  (2022) e2200653. (B02, B08, A06)

Mair V, Paulus I, Groll J, Ryma M, Freeform printing of thermoresponsive poly(2-cyclopropyl-oxazoline) as cytocompatible and on-demand dissolving template of hollow channel networks in cell-laden hydrogels, Biofabrication doi:10.1088/1758-5090/ac57a7  (2022). (B02)

Hauptstein J, Forster L, Nadernezhad A, Groll J, Teßmar J, Blunk T, Tethered TGF-β1 in a Hyaluronic Acid-Based Bioink for Bioprinting Cartilaginous Tissues, International Journal of Molecular Sciences 23(2) (2022). (A02, A06, B02)

Hauptstein J, Forster L, Nadernezhad A, Horder H, Stahlhut P, Groll J, Blunk T, Teßmar J, Bioink Platform Utilizing Dual-stage Crosslinking of Hyaluronic Acid Tailored for Chondrogenic Differentiation of Mesenchymal Stromal Cells, Macromolecular Bioscience n/a(n/a)  2100331. (A02, A06, B02, C02)

2021

Friedrich RP, Cicha I, Alexiou C, Iron Oxide Nanoparticles in Regenerative Medicine and Tissue Engineering, Nanomaterials 11(9) (2021). (B02)

Ryma M, Tylek T, Bloehbaum J, Fernandez R, Boehm C, Blum C, Kastenmueller W, Gasteiger G, Groll J, Translation of Collagen Ultrastructure to Biomaterial Fabrication for Material Independent but Highly Efficient Topographic Immunomodulation, Adv. Mater. doi:10.1002/adma.202101228  (2021). (B02)

Nadernezhad A, Ryma M, Genç H, Cicha I, Jüngst T, Groll J, Melt Electrowriting of Isomalt for High‐Resolution Templating of Embedded Microchannels, Advanced Materials Technologies doi:10.1002/admt.202100221 (2021). (B02)

Horder H, Guaza Lasheras M, Grummel N, Nadernezhad A, Herbig J, Ergun S, Tessmar J, Groll J, Fabry B, Bauer-Kreisel P, Blunk T, Bioprinting and Differentiation of Adipose-Derived Stromal Cell Spheroids for a 3D Breast Cancer-Adipose Tissue Model, Cells 10(4) (2021) 803. (C02, A02, B02, B04)

Genc H, Hazur J, Karakaya E, Dietel B, Bider F, Groll J, Alexiou C, Boccaccini AR, Detsch R, Cicha I, Differential Responses to Bioink-Induced Oxidative Stress in Endothelial Cells and Fibroblasts, Int. J. Mol. Sci. 22(5) (2021) 2358. (B02, A01, B06)

2020

Sun W, Starly B, Daly AC, Burdick JA, Groll J, Skeldon G, Shu W, Sakai Y, Shinohara M, Nishikawa M, Jang J, Cho DW, Nie M, Takeuchi S, Ostrovidov S, Khademhosseini A, Kamm RD, Mironov V, Moroni L, Ozbolat IT, The bioprinting roadmap, Biofabrication 12(2) (2020) 022002. (B02, B04)

Hauptstein J, Bock T, Bartolf-Kopp M, Forster L, Stahlhut P, Nadernezhad A, Blahetek G, Zernecke-Madsen A, Detsch R, Jungst T, Groll J, Tessmar J, Blunk T, Hyaluronic Acid-Based Bioink Composition Enabling 3D Bioprinting and Improving Quality of Deposited Cartilaginous Extracellular Matrix, Adv Healthc Mater 9(15) (2020) 2000737. (A02, A06, B02, B06, Z01)

Dalton PD, Woodfield TBF, Mironov V, Groll J, Advances in Hybrid Fabrication toward Hierarchical Tissue Constructs, Adv Sci (Weinh) 7(11) (2020) 1902953. (A04, B02)

2019

Ryma M, Blohbaum J, Singh R, Sancho A, Matuszak J, Cicha I, Groll J, Easy-to-Prepare Coating of Standard Cell Culture Dishes for Cell-Sheet Engineering Using Aqueous Solutions of Poly(2-n-propyl-oxazoline), ACS Biomater Sci Eng 5(3) (2019) 1509-1517. (B02)

2018

Groll J, Burdick JA, Cho DW, Derby B, Gelinsky M, Heilshorn SC, Jungst T, Malda J, Mironov VA, Nakayama K, Ovsianikov A, Sun W, Takeuchi S, Yoo JJ, Woodfield TBF, A definition of bioinks and their distinction from biomaterial inks, Biofabrication 11(1) (2018) 013001. (A06, B02, B04)

Genc H, Hazur J, Karakaya E, Dietel B, Bider F, Groll J, Alexiou C, Boccaccini AR, Detsch R, Cicha I (2021) Alginate di-Aldehyde-Induced Oxidative Stress in Endothelial Cells and Fibroblasts: Cell-specific Responses, 31st Annual Conference of the European Society for Biomaterials (ESB 2021), 5 to 9 September 2021 (A01, B02, B06)

Hauptstein J, Forster L, Nadernezhad A, Groll J, Blunk T, Teßmar J (2021) Dual-stage Crosslinked Hyaluronic Acid-based Bioink Tailored for Chondrogenic Differentiation of Mesenchymal Stromal Cells, 31st Annual Conference of the European Society for Biomaterials (ESB 2021), 5 to 9 September 2021 (A02, A06, B02)

Horder H, Guaza Lasheras M, Grummel N, Nadernezhad A, Herbig J, Ergün S, Teßmar J, Groll J, Fabry B, Bauer-Kreisel P, Blunk T (2021) A 3D Bioprinted Breast Cancer-Adipose Tissue Model Utilizing Adipose-Derived Stromal Cell Spheroids, 31st Annual Conference of the European Society for Biomaterials (ESB 2021), 5 to 9 September 2021 (A02, B02, B04, C02)

Mair VT, Paulus I, Groll J, Ryma M (2021) Sacrificial thermoresponsive Poly(2-cyclopropyl-oxazoline) as a beneficial material for freeform 3D printing and channel network generation in hydrogels, 31st Annual Conference of the European Society for Biomaterials (ESB 2021), 5 to 9 September 2021 (B02)

Genç H, Hazur J, Karakaya E, Dietel B, Bider F, Groll J, Alexiou C, Boccaccini AR, Detsch R, Cicha I (2021) Alginate di-Aldehyde-Induced Oxidative Stress in Endothelial Cells and Fibroblasts: Cell-specific Responses, September 2021 (Virtual conference)

Hauptstein J, Böck T, Bartolf-Kopp M, Forster L, Stahlhut P, Nadernezhad A, Blahetek G, Zernecke-Madsen A, Detsch R, Jüngst T, Groll J, Teßmar J, Blunk T (2020) Hyaluronic Acid-based Bioink Composition Enabling 3D Bioprinting and Improving Quality of Deposited Cartilaginous Extracellular Matrix, Materials Science and Engineering (MSE), 22 – 25 September 2020 – Web conference

Nadernezhad A, Ryma M, Genc H, Cicha I, Groll J (2020) Design of pre-endothelialized microvascular structures for biofabrication of in vitro tissue models, 5th Bioinspired Materials 2020 Conference, 16 – 19 March 2020 – Web conference

Genç G, Ryma M, Schneidereit D, Nadernezhad A, Groll J, Cicha I (2019) Efficiency of pre-endothelialization by using sacrificial microvessel structures for biofabrication of tissue models, ISBF 2019, Columbus, Ohio, USA

Genç H, Ryma M, Schneidereit D, Nadernezhad A, Groll J, Cicha I (2019) Pre-endothelialization of sacrificial microvessel structures for biofabrication of tissue models, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)

Ryma M, Tylek T, Blöhbaum J, Fernandez R, Groll J (2019) Melt-Electrowritten 3D Scaffolds Based on Aligned Nano-Structured Microfibers for Topography-Mediated Immunomodulation of Human Macrophages, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)

Groll J (2019) 3D Printable Hydrogel-Bioinks: Rheological Implications and the Importance of Controlled Network Formation, TERMIS European Chapter Meeting 2019 27th-31st May 2019, Rhodes, Greece Tissue Engineering Therapies: From Concept to Clinical Translation & Commercialisation

Groll J (2019) Biofabrication – Chances, Challenges and Limits, Euro BioMAT 2019, 08 – 09 May 2019 (Jena-Weimar, Germany)

Groll J (2018) Biofabrication research at FMZ: from solution electrospun meshes to thiol-ene cross-linked hydrogel-bioinks, International Conference on Additive Manufacturing and Biomanufacturing,  2-4 December 2018 (Beijing, China)

Groll J (2018) Bio-ink development for Biofabrication, International Conference on Emerging Healthcare Materials, 15-16 November 2018 (Seoul, South-Korea)

Groll J (2018) SFB/TRR 225: From the Fundamentals of Biofabrication to Functional Tissue Models, International Conference on Biofabrication (ISBF) 2018, 28-31 October 2018 (Würzburg, Germany)

Ryma M, Blöhbaum J, Böhm C, Genc H, Dalton P, Cicha I, Groll J (2018) Prevascularization of Tissue Engineered Constructs via Melt Electrowriting of Thermoresponsive Poly(2-Oxazoline)s, International Conference on Biofabrication (ISBF) 2018, 28-31 October 2018 (Würzburg, Germany)

Groll J (2018) Hydrogels as Bioinks for Biofabrication, MSE conference, 26 – 28 September 2018, (Darmstadt, Germany)

Groll J (2018) Melt Electro Writing at the Department of Functional Materials in Medicine and Dentistry, Annual Meeting of the European Society for Biomaterials,  10-13 September 2018 (Maastricht, Netherlands)

Groll J (2018) Biofabrication: Status of the field and translational perspective, 3D-BioNET-working-event,  18 July 2018 (Freiburg im Breisgau, Germany)

Groll J (2018) Biotinten: 3D-Druckbare Hydrogele als Schlüssel zum BioPrinting, Materialinnovationen 2018 – Vom Material zur Innovation, Konferenz zur Materialforschung des BMBF, 4-6. June 2018, (München, Germany)

Groll J (2018) Hydrogels as “Bio-inks” for Biofabrication, 2nd Theodore von Kármán – Discussion Conference on Materials for Life: Bioinspired and Biomimetic Hydrogels, 15-18 April 2018, (Bensberg, Germany).

Groll J (2018) Bioinks: Concepts, Rheological Considerations and Recent Trends, TERMIS workshop on 3D Printing in Musculoskeletal Tissue Engineering: Bioprinting in Action, 23 March 2018 (Warsaw, Poland)

Groll J (2018) 3D-Druckbare Hydrogele als Tinten für die Biofabrikation, Thüringer Biomaterial Kolloquium, 13. – 15. March 2018 (Zeulenroda, Germany)

Ali Nadernezhad

Previous academic education: Master of Science in Biomedical Engineering (Biomaterials)
Academic Advisor within SFB TRR225: Prof. Dr. Jürgen Groll
Location: Department of functional material in medicine and dentistry, University of Würzburg
Topic of thesis: Pre-endothelialized perfusable microvascular vesselsystems for biofabrication of standardized tissue models
Main techniques and methodologies to be used: Melt Electrowriting, 3D cell culture

Contact: ali.nadernezhad@fmz.uni-wuerzburg.de