A06 | Cell-loaded microgels as mechanical protection and controlled microenvironment for cells in bioinks

Aim of this project is the generation of cell-loaded microgels with uniform size and their establishment as additives in bioinks. The microgels shall on the one side act as mechanical protection from shearforces during printing and thereby extend the biofabrication window towards bioinks with higher viscosity and towards higher shear forces. In addition to that, biochemical functionalization and adjusting microgel degradability shall be used to tailor the microenvironment of the encapsulated cells and control their behavior. Prospectively, this project aims at establishing suspensions of cell-loaded microgels as bioink especially for the simultaneous printing of different cell types.

Prof. Dr. Stephan Förster
Prof. Dr. Jürgen Groll

 

Jürgen Groll

Sophia Löffelsend

Previous academic education: B.Sc. Chemistry (2018, JMU), M.Sc. Biofabrication (2020, JMU), B.Sc. Psychology (~ 2022/23, JMU)
Academic Advisor within SFB TRR225: Prof. Dr. Jürgen Groll
Location: University of Würzburg, Department for functional materials in medicine and dentistry
Topic of thesis: Biofunctional and degradable polymers for cell-loaded microgels
Main techniques and methodologies to be used: Polymer synthesis and characterization, microfluidics

Contact: sophia.loeffelsend@fmz.uni-wuerzburg.de

Anna Meyer

Previous academic education: Bachelor (B.Sc.) and Master of Science (M.Sc.) in Chemistry at the RWTH Aachen
Academic Advisor within SFB TRR225: Prof. Dr. Stephan Förster (and Dr. Stephan Hauschild)
Location: Forschungszentrum Jülich
Topic of thesis: Microfluidic Methods for Biofabrication
Main techniques and methodologies to be used: Microfluidics, photolithography, soft lithography, 3D (bio)printing, CFD simulations, confocal microscopy

Contact: an.meyer@fz-juelich.de

Dr. Stephan Hauschild

Location: Forschungszentrum Jülich, JCNS-1/ICS-1: Neutron Scattering and Soft Matter
Contact: s.hauschild@fz-juelich.de

2022

Ryma M, Genc H, Nadernezhad A, Paulus I, Schneidereit D, Friedrich O, Andelovic K, Lyer S, Alexiou C, Cicha I, Groll J, A print-and-fuse Strategy for Sacrificial Filaments Enables Biomimetically Structured Perfusable Microvascular Networks with Functional Endothelium Inside 3D Hydrogels, Adv Mater doi:10.1002/adma.202200653  (2022) e2200653. (B02, B08, A06)

Heid S, Becker K, Byun J, Biermann I, Neščáková Z, Zhu H, Groll J, Boccaccini AR, Bioprinting with bioactive alginate dialdehyde-gelatin (ADA-GEL) composite bioinks: Time-dependent in-situ crosslinking via addition of calcium-silicate particles tunes in vitro stability of 3D bioprinted constructs, Bioprinting doi:10.1016/j.bprint.2022.e00200  (2022). (B03, A01, A06)

Hauptstein J, Forster L, Nadernezhad A, Groll J, Teßmar J, Blunk T, Tethered TGF-β1 in a Hyaluronic Acid-Based Bioink for Bioprinting Cartilaginous Tissues, International Journal of Molecular Sciences 23(2) (2022). (A02, A06, B02)

Hauptstein J, Forster L, Nadernezhad A, Horder H, Stahlhut P, Groll J, Blunk T, Teßmar J, Bioink Platform Utilizing Dual-stage Crosslinking of Hyaluronic Acid Tailored for Chondrogenic Differentiation of Mesenchymal Stromal Cells, Macromolecular Bioscience n/a(n/a)  2100331. (A02, A06, B02, C02)

2021

Brand JS, Forster L, Bock T, Stahlhut P, Tessmar J, Groll J, Albrecht K, Covalently Crosslinked Pig Gastric Mucin Hydrogels Prepared by Radical-based Chain-Growth and Thiol-ene Mechanisms, Macromol Biosci doi:10.1002/mabi.202100274  (2021) e2100274. (B07, A02, A06)

Förster S, Groll J, Reineke B, Hauschild S, Paulus I, Hazur J, Vollmer M, Tamgüney G, Boccacini A, On-chip-fabrication-and-in-flow-3d-printing-of-cell-laden-microgel-constructs-from-chip-to-scaffold-materials-in-one-integral-process, doi:10.26434/chemrxiv.14564508.v1  (2021). (A06)

Pinzner F, Keller T, Mut J, Bechold J, Seibel J, Groll J, Polyoxazolines with a Vicinally Double-Bioactivated Terminus for Biomacromolecular Affinity Assessment, Sensors 21(9) (2021). (A06, B05)

Blum C, Taskin MB, Shan J, Schilling T, Schlegelmilch K, Tessmar J, Groll J, Appreciating the First Line of the Human Innate Immune Defense: A Strategy to Model and Alleviate the Neutrophil Elastase-Mediated Attack toward Bioactivated Biomaterials, Small 17(13) (2021) 2007551. (A06, A02)

Muir VG, Qazi TH, Shan J, Groll J, Burdick JA, Influence of Microgel Fabrication Technique on Granular Hydrogel Properties, ACS Biomater Sci Eng doi:10.1021/acsbiomaterials.0c01612  (2021). (A02, A06)

2020

Liebscher J, Tessmar J, Groll J, In Situ Polymer Analogue Generation of Azlactone Functions at Poly(oxazoline)s for Peptide Conjugation, Macromol. Chem. Phys. 221(1) (2020) 1900500. (A06, B04)

Hauptstein J, Bock T, Bartolf-Kopp M, Forster L, Stahlhut P, Nadernezhad A, Blahetek G, Zernecke-Madsen A, Detsch R, Jungst T, Groll J, Tessmar J, Blunk T, Hyaluronic Acid-Based Bioink Composition Enabling 3D Bioprinting and Improving Quality of Deposited Cartilaginous Extracellular Matrix, Adv Healthc Mater 9(15) (2020) 2000737. (A02, A06, B02, B06, Z01)

2019

Blohbaum J, Paulus I, Poppler AC, Tessmar J, Groll J, Influence of charged groups on the cross-linking efficiency and release of guest molecules from thiol-ene cross-linked poly(2-oxazoline) hydrogels, J Mater Chem B 7(10) (2019) 1782-1794. (A06)

Berberich O, Blöhbaum J, Hölscher-Doht S, Meffert RH, Teßmar J, Blunk T, Groll J, Catechol-modified poly(oxazoline)s with tunable degradability facilitate cell invasion and lateral cartilage integration, J. Ind. Eng. Chem. 80 (2019) 757-769. (A06, B04)

2018

Groll J, Burdick JA, Cho DW, Derby B, Gelinsky M, Heilshorn SC, Jungst T, Malda J, Mironov VA, Nakayama K, Ovsianikov A, Sun W, Takeuchi S, Yoo JJ, Woodfield TBF, A definition of bioinks and their distinction from biomaterial inks, Biofabrication 11(1) (2018) 013001. (A06, B02, B04)

Reineke B, Paulus I, Hauschild S, Groll, J, Förster S,(2021) All-in-one: On-chip fabrication and in-flow bioprinting of perfusable cell-laden microgel constructs, 6th World Congress 202, Tissue Engineering and Regenerative Medicine International Society (TERMIS2021) 15 – 19 November 2021 (virtual)

Hauptstein J, Forster L, Nadernezhad A, Groll J, Blunk T, Teßmar J (2021) Dual-stage Crosslinked Hyaluronic Acid-based Bioink Tailored for Chondrogenic Differentiation of Mesenchymal Stromal Cells, 31st Annual Conference of the European Society for Biomaterials (ESB 2021), 5 – 9 September 2021 (A02, A06, B02)

Paulus I, Reineke B, Shan J, Hauschild S, Teßmar J, Förster S, Groll J (2021) Bioprinting of microfluidic generated microgels based on a degradable and bioactivated POx/GelPA hybrid system, 31st Annual Conference of the European Society for Biomaterials (ESB 2021), 5 – 9 September 2021 (virtual) (A06, A02)

Reineke B, Förster S, Groll, J, Hauschild S, Paulus I (2021) Advanced Microfluidics for Biofabrication, 6th European Symposium on Biomaterials (6th Euro BioMAT 2021), 05 – 06 May 2021

Groll J (2019) 3D Printable Hydrogel-Bioinks: Rheological Implications and the Importance of Controlled Network Formation, TERMIS European Chapter Meeting 2019 27th-31st May 2019, Rhodes, Greece Tissue Engineering Therapies: From Concept to Clinical Translation & Commercialisation

Groll J (2019) Biofabrication – Chances, Challenges and Limits, Euro BioMAT 2019, 08 – 09 May 2019 (Jena-Weimar, Germany)

Groll J (2018) Biofabrication research at FMZ: from solution electrospun meshes to thiol-ene cross-linked hydrogel-bioinks, International Conference on Additive Manufacturing and Biomanufacturing,  2-4 December 2018 (Beijing, China)

Groll J (2018) Bio-ink development for Biofabrication, International Conference on Emerging Healthcare Materials, 15-16 November 2018 (Seoul, South-Korea)

Groll J (2018) SFB/TRR 225: From the Fundamentals of Biofabrication to Functional Tissue Models, International Conference on Biofabrication (ISBF) 2018, 28-31 October 2018 (Würzburg, Germany)

Groll J (2018) Hydrogels as Bioinks for Biofabrication, MSE conference, 26 – 28 September 2018, (Darmstadt, Germany)

Groll J (2018) Melt Electro Writing at the Department of Functional Materials in Medicine and Dentistry, Annual Meeting of the European Society for Biomaterials,  10 – 13 September2018 (Maastricht, Netherlands)

Groll J (2018) Biofabrication: Status of the field and translational perspective, 3D-BioNET-working-event,  18 July 2018 (Freiburg im Breisgau, Germany)

Groll J (2018) Biotinten: 3D-Druckbare Hydrogele als Schlüssel zum BioPrinting, Materialinnovationen 2018 – Vom Material zur Innovation, Konferenz zur Materialforschung des BMBF, 4. – 6. June 2018, (München, Germany)

Hauptstein J, Böck T, Bartolf-Kopp M, Forster L, Stahlhut P, Nadernezhad A, Blahetek G, Zernecke-Madsen A, Detsch R, Jüngst T, Groll J, Teßmar J, Blunk T (2020) Hyaluronic Acid-based Bioink Composition Enabling 3D Bioprinting and Improving Quality of Deposited Cartilaginous Extracellular Matrix, Materials Science and Engineering (MSE), 22 – 25 September 2020 – Web conference

Groll J (2018) Hydrogels as “Bio-inks” for Biofabrication, 2nd Theodore von Kármán – Discussion Conference on Materials for Life: Bioinspired and Biomimetic Hydrogels, 15 – 18 April 2018, (Bensberg, Germany).

Groll J (2018) Bioinks: Concepts, Rheological Considerations and Recent Trends, TERMIS workshop on 3D Printing in Musculoskeletal Tissue Engineering: Bioprinting in Action, 23 March 2018 (Warsaw, Poland)

Groll J (2018) 3D-Druckbare Hydrogele als Tinten für die Biofabrikation, Thüringer Biomaterial Kolloquium, 13. – 15. March 2018 (Zeulenroda, Germany)

Paulus I, Reineke B, Hauschild S, Förster S, Groll J (2020) Cell-loaded Microgels as mechanical Protection and controlled Microenvironment for Cells in Bioinks, 5th Bioinspired Materials 2020 Conference, 16 – 19 March 2020 – Web conference

Paulus I, Bloehbaum J, Tessmar J, Groll J (2019) Visible Light Cross-linked Poly(2-oxazoline) (POx)-based Hydrogels via Thiol-Ene Reaction, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)

Herbig J, Shan J, Jüngst T, Albrecht K, Groll J (2019) Microgel based bioinks, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)

Reineke B, Hauschild S, Förster S (2018) Development of microfluidic devices for the encapsulation of cells, Jülich Soft Matter Days 2018 20-23 November 2018 (Jülich, Germany)

Ilona Paulus

Previous academic education: Master of Science in chemistry
Academic Advisor within SFB TRR225: Prof. Dr. Jürgen Groll
Location: University of Würzburg, Department for functional materials in medicine and dentistry
Topic of thesis: Multifunctional POx-based building blocks for bioinks
Main techniques and methodologies to be used: Chemical synthesis, Microfluidic, 3D-Printing

Contact: ilona.paulus@fmz.uni-wuerzburg.de

Benjamin Reineke

Previous academic education: Polymer Science M.Sc. @ Uni Bayreuth
Academic Advisor within SFB TRR225: Prof. Dr. Stephan Förster
Location: Forschungszentrum Jülich
Topic of thesis: Microfluidic chips for the encapsulation of cells
Main techniques and methodologies to be used: Microfluidic chip design, soft lithography, preparation of colloids using microfluidics

Contact: b.reineke@fz-juelich.de