C04 | Biofabrication of cellularized and by the AV-loop vascularized tissue container for the transplantation of cells producing therapeutic proteins

Aim of the project is the development of a broadly applicable transplantable tissue container which enables the in vivo production of recombinant therapeutic proteins, e.g. antibodies. For this a container shell will be filled with two cell doted matrix cylinders structured with tubes. The cylinders contains a bed in which a AV loop can be placed which sprouts into the tube system to ensure the vascularization of the container and producer cell maintenance. Container vascularization in the bioreactor, container immune tolerance and container evaluation in pre-clinical models are the subjects of future funding periods.

Prof. Dr. Dr. h.c. Raymund Horch
Prof. Dr. Harald Wajant

Prof. Dr. Dr. Raymund E. Horch

Friedrich-Alexander-University Erlangen-Nürnberg (FAU), Department of Plastic and Hand Surgery University Hospital of Erlangen
Contact: Raymund.Horch@uk-erlangen.de

HaMiPla – Best paper award 2018 for Prof. Raymund E. Horch

Prof. Dr. Harald Wajant

University Hospital Würzburg, Division of Molecular Internal Medicine Department for Internal Medicine II
Contact: wajant_h1@ukw.de

 

 

Stefanie Heltmann-Meyer

Previous academic education: Master
Academic Advisor within SFB TRR225: Prof. Dr. Dr. Raymund E. Horch
Location: Friedrich-Alexander-University Erlangen-Nürnberg (FAU), Department of Plastic and Hand Surgery University Hospital of Erlangen
Topic of thesis: Generation of bioactive tissue containers in the rat AV loop model
Main techniques and methodologies to be used: microsurgical operations (rat), histological and molecular biological analyses of the explants

Contact: stefanie.heltmann-meyer@uk-erlangen.de

 

Hanna Amouei

Previous academic education: Master
Academic Advisor within SFB TRR225: Prof. Dr. Harald Wajant
Location: University Hospital Würzburg, Division of Molecular Internal Medicine Department for Internal Medicine II
Topic of thesis: Development of structured, cell-doted matrices for the “in vivo” production of biologicals
Main techniques and methodologies to be used: GpL fusion proteins as tracers, Development of producer cell lines, Ink, structure  and print protocol evaluation

Contact: hanna.amouei@uni-wuerzburg.de

 

Steiner D, Mutschall H, Winkler S, Horch RE, Arkudas A, The Adipose-Derived Stem Cell and Endothelial Cell Coculture System-Role of Growth Factors?, Cells 10(8) (2021). (C04)

Heltmann-Meyer S, Steiner D, Muller C, Schneidereit D, Friedrich O, Salehi S, Engel FB, Arkudas A, Horch RE, Gelatin methacryloyl is a slow degrading material allowing vascularization and long-term usein vivo, Biomed Mater 16(6) (2021). (B03, B08, C01, C04)

Winkler S, Mutschall H, Biggemann J, Fey T, Greil P, Korner C, Weisbach V, Meyer-Lindenberg A, Arkudas A, Horch RE, Steiner D, Human Umbilical Vein Endothelial Cell Support Bone Formation of Adipose-Derived Stem Cell-Loaded and 3D-Printed Osteogenic Matrices in the Arteriovenous Loop Model, Tissue Eng Part A 27(5-6) (2021) 413-423. (C04, C03)

 Steiner D, Winkler S, Heltmann-Meyer S, Trossmann V, Fey T, Scheibel T, Horch RE, Arkudas A, Enhanced vascularization andde novotissue formation in hydrogels made of engineered RGD-tagged spider silk proteins in the arteriovenous loop model, Biofabrication doi:10.1088/1758-5090/ac0d9b  (2021). (C01, C04)

Hessenauer MET, Vaghela R, Korner C, von Horsten S, Pobel C, Gage D, Muller C, Salehi S, Horch RE, Arkudas A, Watching the vessels grow – establishment of intravital microscopy in the AV-loop rat model, Tissue Eng Part C Methods doi:10.1089/ten.TEC.2021.0024  (2021). (C04, B03)

Tolksdorf J, Horch RE, Gruner JS, Schmid R, Kengelbach-Weigand A, Schubert DW, Werner S, Schneidereit D, Friedrich O, Ludolph I, Size matters-in vitro behaviour of human fibroblasts on textured silicone surfaces with different pore sizes, J. Mater. Sci.: Mater. Med. 31(2) (2020) 23. (C04, B08, A07, C03)

Mutschall H, Winkler S, Weisbach V, Arkudas A, Horch RE, Steiner D, Bone tissue engineering using adipose-derived stem cells and endothelial cells: Effects of the cell ratio, J. Cell. Mol. Med. 24(12) (2020) 7034-7043. (C04, C03)

Steiner D, Lang G, Fischer L, Winkler S, Fey T, Greil P, Scheibel T, Horch RE, Arkudas A, Intrinsic Vascularization of Recombinant eADF4(C16) Spider Silk Matrices in the Arteriovenous Loop Model, Tissue Eng Part A 25(21-22) (2019) 1504-1513. (C04, A07, C01, C03)

Weigand A, Horch RE, Boos AM, Beier JP, Arkudas A, The Arteriovenous Loop: Engineering of Axially Vascularized Tissue, Eur. Surg. Res. 59(3-4) (2018) 286-299. (C03, C04)

Steiner D, Lingens L, Fischer L, Kohn K, Detsch R, Boccaccini AR, Fey T, Greil P, Weis C, Beier JP, Horch RE, Arkudas A, Encapsulation of Mesenchymal Stem Cells Improves Vascularization of Alginate-Based Scaffolds, Tissue Eng Part A 24(17-18) (2018) 1320-1331. (C03, C04, A01, B06, B03) Front Cover for Tissue Engineering Part A

Rottensteiner-Brandl U, Detsch R, Sarker B, Lingens L, Kohn K, Kneser U, Bosserhoff AK, Horch RE, Boccaccini AR, Arkudas A, Encapsulation of Rat Bone Marrow Derived Mesenchymal Stem Cells in Alginate Dialdehyde/Gelatin Microbeads with and without Nanoscaled Bioactive Glass for In Vivo Bone Tissue Engineering, Materials (Basel) 11(10) (2018) 1880. (C04, A01, C03)

Horch RE, Weigand A, Wajant H, Groll J, Boccaccini AR, Arkudas A, [Biofabrication: new approaches for tissue regeneration], Handchir Mikrochir Plast Chir 50(2) (2018) 93-100. (C04)

Winkler S, Steiner D, Biggemann J, Fey T, Greil P, Körner C, Weisbach V, Meyer-Lindenberg A, Mutschall H, Arkudas A and Horch RE (2019) Intrinsic vascularization of ADSC-loaded hydrogel scaffolds in the rat AV loop model, The 11th EPSRC Meeting, 23-25 August 2019, Munich, Germany, Klinikum rechts der Isar, Technische Universität München (München, Germany)

Heltmann-Meyer S (2019) Biofabrikation zellularisierter und im AV Loop vaskularisierter Gewebecontainer für die Transplantation wirkstoffproduzierender Zellen, 3. DAM Wissenschaftsakademie, 12 – 13 April 2019 (Erlangen, Germany)

Peddi A, Steiner D, Weigand A, Lingens L, Fischer L, Köhn K, Detsch R, Boccaccini AR, Fey T, Greil P, Weis C, Beier JP, Horch RE, Arkudas A. (2018) Encapsulation of mesenchymal stem cells improves vascularization of alginate-based scaffolds,  The 10th EPSRC Meeting, 23 – 25 August 2018 (Hamburg, Germany)

Heltmann-Meyer S, Steiner D, Lang G, Winkler S, Fischer L, Fey T, Greil P, Scheibel T, Horch RE, Arkudas A. (2018) Intrinsic Vascularization of Recombinant eADF4(C16) Spider Silk fibrous matrices in the Arteriovenous Loop Model, 10th EPSRC Meeting, 23 – 25 August 2018 (Hamburg, Germany)

Trossmann V, Steiner D, Heltmann-Meyer S, Winkler S, Amouei H, Wajant H, Fey T, Greil P, Scheibel T, Arkudas A, Horch RE, Establishment of a fiber-based and RGD-modified spider silk for the generation of a drug-producing tissue container, 5th Bioinspired Materials 2020 Conference, 16 – 19 March 2020 – Web conference

Weigand A, Boos AM, An R, Strissel PL, Schmid R, Robering JW, Al-Abboodi M, Detsch R, Dalton PD, Youssef A , Boccaccini AR, Beckmann MW, Bäuerle T, Strick R, Horch RE (2018) From Tissue Engineering to Tumor Engineering –  Application of the arteriovenous loop model for the generation of a fully vascularized tumor in an animal model, 2018 TERMIS World Congress, 4-7 September 2018 (Kyoto, Japan)