C04 | Biofabrication of cellularized and by the AV-loop vascularized tissue container for the transplantation of cells producing therapeutic proteins

Aim of the project is the development of a broadly applicable transplantable tissue container which enables the in vivo production of recombinant therapeutic proteins, e.g. antibodies. For this a container shell will be filled with two cell doted matrix cylinders structured with tubes. The cylinders contains a bed in which a AV loop can be placed which sprouts into the tube system to ensure the vascularization of the container and producer cell maintenance. Container vascularization in the bioreactor, container immune tolerance and container evaluation in pre-clinical models are the subjects of future funding periods.

Prof. Dr. Dr. h.c. Raymund Horch
Prof. Dr. Harald Wajant


Rottensteiner-Brandl U, Detsch R, Sarker B, Lingens L, Köhn K, Kneser U, Bosserhoff AK, Horch RE, Boccaccini AR, Arkudas A. (2018) Encapsulation of Rat Bone Marrow Derived Mesenchymal Stem Cells in Alginate Dialdehyde/Gelatin Microbeads with and without Nanoscaled Bioactive Glass for In Vivo Bone Tissue Engineering. Materials (Basel). 2018 Oct 1;11(10). pii: E1880. doi: 10.3390/ma11101880.

Steiner D, Lingens L, Fischer L, Köhn K, Detsch R, Boccaccini AR, Fey T, Greil P, Weis C, Beier J, Horch RE, Arkudas A. (2018) Encapsulation of mesenchymal stem cells improves vascularization of alginate-based scaffolds. Tissue Eng Part A. 2018 Mar 20. doi: 10.1089/ten.TEA.2017.0496.      Front Cover for Tissue Engineering Part A

Stefanie Heltmann-Meyer

Previous academic education: Master
Academic Advisor within SFB TRR225: Prof. Dr. Dr. Raymund E. Horch
Location: Friedrich-Alexander-University Erlangen-Nürnberg (FAU), Department of Plastic and Hand Surgery University Hospital of Erlangen
Topic of thesis: Generation of bioactive tissue containers in the rat AV loop model
Main techniques and methodologies to be used: microsurgical operations (rat), histological and molecular biological analyses of the explants

Contact: stefanie.heltmann-meyer@uk-erlangen.de


Hanna Amouei

Previous academic education: Master
Academic Advisor within SFB TRR225: Prof. Dr. Harald Wajant
Location: University Hospital Würzburg, Division of Molecular Internal Medicine Department for Internal Medicine II
Topic of thesis: Development of structured, cell-doted matrices for the “in vivo” production of biologicals
Main techniques and methodologies to be used: GpL fusion proteins as tracers, Development of producer cell lines, Ink, structure  and print protocol evaluation

Contact: hanna.amouei@uni-wuerzburg.de