B08 | Time-resolved biophotonics approach cellular signaling, cell-matrix interactions and matrix remodeling mechanisms in biofabricated constructs

In this optical engineering and bioprocess engineering project we aim to develop new systems to study in situ maturation and regulation of cell-matrix junctions of biofabricated constructs over long time periods. To this end, (i) we will advance high resolution/depth penetrating multiphoton- and light sheet technology towards a 2-photon single plane illumination microscopy (2P-SPIM), and (ii) design and engineer precision positioned mobile mini-bioreactors. We will validate these systems for selected bioconstructs within the consortium regarding (iii) visualization of 3D spatial patterning of focal adhesion proteins between cell and matrix as well as matrix production during long-term maturation of constructs.

Prof. Dr. Dr. Andreas Beilhack
Prof. Dr. Dr. Oliver Friedrich 

Dominik Schneidereit

Previous academic education: Master of Science
Academic Advisor within SFB TRR225: Prof. Dr. Dr. Oliver Friedrich
Location: FAU-Erlangen, Institute of Medical Biotechnology
Topic: Neuartige optomechatronische Kombinationstechnologien im Cardio-muskulären Umfeld für Medizin und Lebenswissenschaften
Main techniques and methodologies to be used: Multi photon light sheet microscopy, Optics simulation, 3D CAD and CAM, FFF printing

Contact: dominik.schneidereit@fau.de

Dr. Zeinab Mokhtari

Previous academic education: Postdoctoral Fellow
Academic Advisor within SFB TRR225: Prof. Dr. Andreas Beilhack
Location: University Hospital of Würzburg, Department of Medicine II
Topic: Time-resolved biophotonics approach for cellular signaling, cell-matrix interactions and matrix remodeling mechanisms in biofabricated constructs
Main techniques and methodologies to be used: Light sheet fluorescence microscopy, Two-photon microscopy, multiphoton light sheet fluorescence microscopy

Contact: Mokhtarias_Z@ukw.de

Heltmann-Meyer S, Steiner D, Muller C, Schneidereit D, Friedrich O, Salehi S, Engel FB, Arkudas A, Horch RE, Gelatin methacryloyl is a slow degrading material allowing vascularization and long-term usein vivo, Biomed Mater 16(6) (2021). (B03, B08, C01, C04)

Vielreicher M, Bozec A, Schett G, Friedrich O, Murine Metatarsus Bone and Joint Collagen-I Fiber Morphologies and Networks Studied With SHG Multiphoton Imaging, Front. Bioeng. Biotechnol. 9 (2021) 608383. (B08)

Spörrer M, Kah D, Gerum RC, Reischl B, Huraskin D, Dessalles CA, Schneider W, Goldmann WH, Herrmann H, Thievessen I, Clemen CS, Friedrich O, Hashemolhosseini S, Schröder R, Fabry B, The desmin mutation R349P increases contractility and fragility of stem cell-generated muscle micro-tissues, bioRxiv (2021) 2021.07.23.453481. (A01, B08, C02)

Shafiee A, Cavalcanti AS, Saidy NT, Schneidereit D, Friedrich O, Ravichandran A, De-Juan-Pardo EM, Hutmacher DW, Convergence of 3D printed biomimetic wound dressings and adult stem cell therapy, Biomaterials 268 (2021) 120558. (B08)

Schneidereit D, Brollochs A, Ritter P, Kreiss L, Mokhtari Z, Beilhack A, Kronke G, Ackermann JA, Faas M, Gruneboom A, Schurmann S, Friedrich O, An advanced optical clearing protocol allows label-free detection of tissue necrosis via multiphoton microscopy in injured whole muscle, Theranostics 11(6) (2021) 2876-2891. (B08)

Kah D, Winterl A, Přechová M, Schöler U, Schneider W, Friedrich O, Gregor M, Fabry B, A low-cost uniaxial cell stretcher for six parallel wells, HardwareX 9 (2021). (A01, B08, C02)

Horvat S, Yu Y, Manz H, Keller T, Beilhack A, Groll J, Albrecht K, Nanogels as Antifungal‐Drug Delivery System Against Aspergillus Fumigatus, Advanced NanoBiomed Research 1(5) (2021) 2000060. (B07, B08)

Distler T, Polley C, Shi F, Schneidereit D, Ashton MD, Friedrich O, Kolb JF, Hardy JG, Detsch R, Seitz H, Boccaccini AR, Electrically Conductive and 3D-Printable Oxidized Alginate-Gelatin Polypyrrole:PSS Hydrogels for Tissue Engineering, Adv Healthc Mater 10 (2021) 2001876. (B08)

Distler T, Kretzschmar L, Schneidereit D, Girardo S, Goswami R, Friedrich O, Detsch R, Guck J, Boccaccini AR, Budday S, Mechanical properties of cell- and microgel bead-laden oxidized alginate-gelatin hydrogels, Biomater. Sci. 9(8) (2021) 3051-3068. (B06, B08)

Weizel A, Distler T, Schneidereit D, Friedrich O, Brauer L, Paulsen F, Detsch R, Boccaccini AR, Budday S, Seitz H, Complex mechanical behavior of human articular cartilage and hydrogels for cartilage repair, Acta Biomater 118 (2020) 113-128. (B08)

Tolksdorf J, Horch RE, Gruner JS, Schmid R, Kengelbach-Weigand A, Schubert DW, Werner S, Schneidereit D, Friedrich O, Ludolph I, Size matters-in vitro behaviour of human fibroblasts on textured silicone surfaces with different pore sizes, J. Mater. Sci.: Mater. Med. 31(2) (2020) 23. (C04, B08, A07, C03)

No YJ, Tarafder S, Reischl B, Ramaswamy Y, Dunstan C, Friedrich O, Lee CH, Zreiqat H, High-Strength Fiber-Reinforced Composite Hydrogel Scaffolds as Biosynthetic Tendon Graft Material, ACS Biomater Sci Eng 6(4) (2020) 1887-1898. (B08)

Hazur J, Detsch R, Karakaya E, Kaschta J, Tessmar J, Schneidereit D, Friedrich O, Schubert DW, Boccaccini AR, Improving alginate printability for biofabrication: establishment of a universal and homogeneous pre-crosslinking technique, Biofabrication 12(4) (2020) 045004. (A01, A02, A07, B06, B08)

Distler T, Solisito AA, Schneidereit D, Friedrich O, Detsch R, Boccaccini AR, 3D printed oxidized alginate-gelatin bioink provides guidance for C2C12 muscle precursor cell orientation and differentiation via shear stress during bioprinting, Biofabrication 12(4) (2020) 045005. (A01, B06, B08)

Cordes S, Mokhtari Z, Bartosova M, Mertlitz S, Riesner K, Shi Y, Mengwasser J, Kalupa M, McGeary A, Schleifenbaum J, Schrezenmeier J, Bullinger L, Diaz-Ricart M, Palomo M, Carrreras E, Beutel G, Schmitt CP, Beilhack A, Penack O, Endothelial damage and dysfunction in acute graft-versus-host disease, Haematologica doi:10.3324/haematol.2020.253716 (2020). (B08)

Amich J, Mokhtari Z, Strobel M, Vialetto E, Sheta D, Yu Y, Hartweg J, Kalleda N, Jarick KJ, Brede C, Jordan-Garrote AL, Thusek S, Schmiedgen K, Arslan B, Pinnecker J, Thornton CR, Gunzer M, Krappmann S, Einsele H, Heinze KG, Beilhack A, Three-Dimensional Light Sheet Fluorescence Microscopy of Lungs To Dissect Local Host Immune-Aspergillus fumigatus Interactions, mBio 11(1) (2020) 02752-19. (B08)

Ritter P, Bye LJ, Finol-Urdaneta RK, Lesko C, Adams DJ, Friedrich O, Gilbert DF, A method for high-content functional imaging of intracellular calcium responses in gelatin-immobilized non-adherent cells, Exp Cell Res 395(2) (2020) 112210. (B08)

Friedrich O (2020) Synthetic material could help heal injured tendons and ligaments, The University of Sydney News 14.04.2020 (2020) (B08)

Schneidereit D, Schurmann S, Friedrich O, PiezoGRIN: A High-Pressure Chamber Incorporating GRIN Lenses for High-Resolution 3D-Microscopy of living Cells and Tissues, Adv Sci (Weinh) 6(4) (2019) 1801453. (B08)

Kahl M, Gertig M, Hoyer P, Friedrich O, Gilbert DF, Ultra-Low-Cost 3D Bioprinting: Modification and Application of an Off-the-Shelf Desktop 3D-Printer for Biofabrication, Front. Bioeng. Biotechnol. 7 (2019) 184. (B08)

Friedrich O (2018) Taking a deep look’ – Advanced 2-Photon Technologies to study Cell-Matrix Architecture in Tissue Engineering, Regenerative Medicine and  Organ Disease, Special Seminar, The University of Sydney, invited speaker, https://wordvine.sydney.edu.au/files/2889/22596/, 12 December 2018 (Sydney, Autralia)

Schneidereit D, Distler T, Meinert C, Carlé B, Detsch R, Schürmann S, Hutmacher D, Boccaccini AR, Friedrich O (2020) Multiphoton Microscopy: A powerful tool to reveal cellular organization and morphology within bioengineered constructs in 3D, 5th Bioinspired Materials 2020 Conference, 16 – 19 March 2020 – Web conference

Schneidereit D, Sulistio AA, Bröllochs A, Lu K, Distler T, Schürmann S, Detsch R, Dendorfer A, Boccaccini AR, Friedrich O (2018) 3D Tissue Substructure: Using Multi-Photon Excitation to Reveal Cellular 3D Morphology of Natural and Engineered Tissue in Depth, Annual Conference of the International Society for Biofabrication (ISBF), 28-31 October, 2018 (Würzburg, Germany)