B08 | Time-resolved biophotonics approach cellular signaling, cell-matrix interactions and matrix remodeling mechanisms in biofabricated constructs

In this optical engineering and bioprocess engineering project we aim to develop new systems to study in situ maturation and regulation of cell-matrix junctions of biofabricated constructs over long time periods. To this end, (i) we will advance high resolution/depth penetrating multiphoton- and light sheet technology towards a 2-photon single plane illumination microscopy (2P-SPIM), and (ii) design and engineer precision positioned mobile mini-bioreactors. We will validate these systems for selected bioconstructs within the consortium regarding (iii) visualization of 3D spatial patterning of focal adhesion proteins between cell and matrix as well as matrix production during long-term maturation of constructs.

Prof. Dr. Dr. Andreas Beilhack
Prof. Dr. Dr. Oliver Friedrich 

Dominik Schneidereit

Previous academic education: Master of Science
Academic Advisor within SFB TRR225: Prof. Dr. Dr. Oliver Friedrich
Location: FAU-Erlangen, Institute of Medical Biotechnology
Topic: Neuartige optomechatronische Kombinationstechnologien im Cardio-muskulären Umfeld für Medizin und Lebenswissenschaften
Main techniques and methodologies to be used: Multi photon light sheet microscopy, Optics simulation, 3D CAD and CAM, FFF printing

Contact: dominik.schneidereit@fau.de

Dr. Zeinab Mokhtari

Previous academic education: Postdoctoral Fellow
Academic Advisor within SFB TRR225: Prof. Dr. Andreas Beilhack
Location: University Hospital of Würzburg, Department of Medicine II
Topic: Time-resolved biophotonics approach for cellular signaling, cell-matrix interactions and matrix remodeling mechanisms in biofabricated constructs
Main techniques and methodologies to be used: Light sheet fluorescence microscopy, Two-photon microscopy, multiphoton light sheet fluorescence microscopy

Contact: Mokhtarias_Z@ukw.de

Distler T, Polley C, Shi F, Schneidereit D, Ashton MD, Friedrich O, Kolb JF, Hardy JG, Detsch R, Seitz H, Boccaccini AR, Electrically Conductive and 3D-Printable Oxidized Alginate-Gelatin Polypyrrole:PSS Hydrogels for Tissue Engineering, Adv Healthc Mater doi:10.1002/adhm.202001876  (2021) e2001876.

Schneidereit D, Bröllochs A, Ritter P, Kreiß L, Mokhtari Z, Beilhack A, Krönke G, Ackermann JA, Faas M, Grüneboom A, Schürmann S, Friedrich O (2021) An advanced optical clearing protocol allows label-free detection of tissue necrosis via multiphoton microscopy in injured whole muscle, Theranostics 2021; 11(6): 2876-2891.

Kah D, Winterl A, Přechová M, Schöler U, Schneider W, Friedrich O, Gregor M, Fabry B (2021) A low-cost uniaxial cell stretcher for six parallel wells    HardwareX, e00162, ISSN 2468-0672, Volume 9, April 2021, e00162

Distler T, Kretzschmar L, Schneidereit D, Girardo S, Goswami R, Friedrich O, Detsch R, Guck J, Boccaccini AR, Budday S (2021) Mechanical properties of cell- and microgel bead-laden oxidized alginate-gelatin hydrogels, Biomater. Sci., 2021, Advance Article

Abbas Shafiee, Amanda S. Cavalcanti, Navid T. Saidy, Dominik Schneidereit, Oliver Friedrich, Akhilandeshwari Ravichandran, Elena Juan Pardo, Dietmar W. Hutmacher (2020) Convergence of 3D Printed Biomimetic Wound Dressings and Adult Stem Cell Therapy, Biomaterials, 2020, 120558, ISSN 0142-9612

Weizel A, Distler T, Schneidereit D, Friedrich O, Bräuer L, Paulsen F, Detsch R, Boccaccini AR, Budday S, Seitz H (2020) Complex mechanical behavior of human articular cartilage and hydrogels for cartilage repair, Acta Biomaterialia, 2020, ISSN 1742-7061

Ritter P, Byec LJ, Finol-Urdanet RK, Lesko C, Adams DJ, Friedrich O, Gilbert DF (2020) A method for high-content functional imaging of intracellular calcium responses in gelatin-immobilized non-adherent cells Experimental Cell Research 395 (2020) 112210

Distler A, Sulistio D, Schneidereit O, Friedrich R, Detsch, Boccaccini AR (2020) 3D printed oxidized alginate-gelatin bioink provides guidance for C2C12muscle precursor cell orientation and differentiation viashear stress during bioprinting, Biofabrication 12 (2020)045005.

Hazur J, Detsch R, Karakaya E, Kaschta J, Tessmar J, Schneidereit D, Friedrich O, Schubert D, Boccaccini AR (2020) Improving alginate printability for biofabrication: establishment of a universal and homogeneous pre-crosslinking technique, Biofabrication in press

Friedrich O (2020) Synthetic material could help heal injured tendons and ligaments, The University of Sydney News 14.04.2020

No YJ, Tarafder S, Reischl B, Ramaswamy Y, Dunstan C, Friedrich O, Lee C, Zreiqat H (2020) High-strength fiber-reinforced composite hydrogel scaffolds as biosynthetic tendon graft material, ACS Biomater. Sci. Eng. 2020, 6, 4, 1887-1898

Tolksdorf J, Horch RE, Grüner JS, Schmid R, Kengelbach-Weigand A, Schubert DW, Werner S, Schneidereit D, Friedrich O, Ludolph I (2020) Size matters-in vitro behaviour of human fibroblasts on textured silicone surfaces with different pore sizes, J Mater Sci Mater Med. 31(2):23. 

Kahl M, Gertig M, Hoyer P, Friedrich O, Gilbert DF (2019) Ultra-Low-Cost 3D Bioprinting: Modification and Application of an Off-the-Shelf Desktop 3D-Printer for Biofabrication, Front. Bioeng. Biotechnol., 31 July 2019

Schneidereit D, Schürmann S, Friedrich O (2018) PiezoGRIN: a high-pressure chamber incorporating GRIN lenses for high-resolution 3D-microscopy of living cells and tissues, Advanced Science, https://doi.org/10.1002/advs.201801453

Friedrich O (2018) Taking a deep look’ – Advanced 2-Photon Technologies to study Cell-Matrix Architecture in Tissue Engineering, Regenerative Medicine and  Organ Disease, Special Seminar, The University of Sydney, invited speaker, https://wordvine.sydney.edu.au/files/2889/22596/, 12 December 2018 (Sydney, Autralia)

Schneidereit D, Distler T, Meinert C, Carlé B, Detsch R, Schürmann S, Hutmacher D, Boccaccini AR, Friedrich O (2020) Multiphoton Microscopy: A powerful tool to reveal cellular organization and morphology within bioengineered constructs in 3D, 5th Bioinspired Materials 2020 Conference, 16 – 19 March 2020 – Web conference

Schneidereit D, Sulistio AA, Bröllochs A, Lu K, Distler T, Schürmann S, Detsch R, Dendorfer A, Boccaccini AR, Friedrich O (2018) 3D Tissue Substructure: Using Multi-Photon Excitation to Reveal Cellular 3D Morphology of Natural and Engineered Tissue in Depth, Annual Conference of the International Society for Biofabrication (ISBF), 28-31 October, 2018 (Würzburg, Germany)