B07 | Development of a micro particle sensor system to establish correlations between mechanical stress and cell functionality during biofabrication

Hydrodynamic forces during printing can cause enduring damage to living cells, their strength is however largely unknown. Here we will develop sensor particles (microcapsules, microgels) which in combination with computer models will close this knowledge gap. The sensor particles will give insight into the mechanical deformation while the closely corresponding simulations will allow us to calculate resulting mechanical stresses. Finally, by comparing with living cells we will be able to establish correlations between deformation, stress and cell damage during bioprinting.

Dr. Krystyna Albrecht
Prof. Dr. Stephan Gekle

Sebastian Müller

Previous academic education: M. Sc. in Physics: “Simulation of Stem Cells in Shear Thinning Bioinks”
Academic Advisor within SFB/TRR 225: Prof. Dr. Stephan Gekle
Location: University of Bayreuth, Theoretical Physics VI
Topic of thesis: Computational estimate of cell damage in bioprinting processes
Main techniques and methodologies to be used: Lattice Boltzmann Simulations, Immersed-Boundary method, Shear thinning fluid models, different cell models

Contact: sebastian.j.mueller@uni-bayreuth.de

Jessica Brand

Previous academic education: 2015, Bachelor in Chemistry: Pikosekunden-zeitaufgelöste Photoionisation von 1,8-Naphthalimid,2017, Master in Chemistry: Synthese und Charakterisierung von Donor- und Akzeptor-substituierten Porphyrinen
Academic Advisor within SFB/TRR 225: Dr. Krystyna Albrecht
Location: University of Würzburg, Department for Functional Materials in Medicine and Dentistry
Topic of thesis: „Comparison of different synthesized microgels for biofabrication“
Main techniques and methodologies to be used: Chemical synthesis, Emulsion technique, Microfluidic, 3D printing

Contact: Jessica.brand@fmz.uni-wuerzburg.de

Muller SJ, Weigl F, Bezold C, Bacher C, Albrecht K, Gekle S, A hyperelastic model for simulating cells in flow, Biomech Model Mechanobiol 20(2) (2021) 509-520. (B07)

Horvat S, Yu Y, Manz H, Keller T, Beilhack A, Groll J, Albrecht K, Nanogels as Antifungal‐Drug Delivery System Against Aspergillus Fumigatus, Advanced NanoBiomed Research 1(5) (2021) 2000060. (B07, B08)

Bächer C, Graessel K, Gekle S, Rayleigh–Plateau instability of anisotropic interfaces. Part 2. Limited instability of elastic interfaces, J. Fluid Mech. 910 (2021) A47. (B07)

Muller SJ, Mirzahossein E, Iftekhar EN, Bacher C, Schrufer S, Schubert DW, Fabry B, Gekle S, Flow and hydrodynamic shear stress inside a printing needle during biofabrication, PLoS One 15(7) (2020) 0236371. (B07, A01, A07)

Lehmann M, Muller SJ, Gekle S, Efficient viscosity contrast calculation for blood flow simulations using the lattice Boltzmann method, Int. J. Numer. Methods Fluids 92(11) (2020) 1463-1477. (B07)

Bacher C, Bender M, Gekle S, Flow-accelerated platelet biogenesis is due to an elasto-hydrodynamic instability, Proc. Natl. Acad. Sci. U. S. A. 117(32) (2020) 18969-18976. (B07)

Neubauer JW, Hauck N, Mannel MJ, Seuss M, Fery A, Thiele J, Mechanoresponsive Hydrogel Particles as a Platform for Three-Dimensional Force Sensing, ACS Appl Mater Interfaces 11(29) (2019) 26307-26313. (B07)

Daddi-Moussa-Ider A, Kurzthaler C, Hoell C, Zottl A, Mirzakhanloo M, Alam MR, Menzel AM, Lowen H, Gekle S, Frequency-dependent higher-order Stokes singularities near a planar elastic boundary: Implications for the hydrodynamics of an active microswimmer near an elastic interface, Phys. Rev. E 100(3-1) (2019) 032610. (B07)

Bacher C, Gekle S, Computational modeling of active deformable membranes embedded in three-dimensional flows, Phys. Rev. E 99(6-1) (2019) 062418. (B07)

Nait-Ouhra A, Guckenberger A, Farutin A, Ez-Zahraouy H, Benyoussef A, Gekle S, Misbah C, Lateral vesicle migration in a bounded shear flow: Viscosity contrast leads to off-centered solutions, Phys. Rev. Fluids 3(12) (2018) 123601. (B07)

Bezold C, Bächer C, Gekle S. (2018) Simulations of stem cells in microjets, Deutsche Physikalische Gesellschaft (DPG), DPG Spring Meeting Berlin, March 11 – 16, 2018 (Berlin, Germany)

Müller SJ, Bezold C, Gekle S (2019) Numerical Investigation of Cell Deformation during Bioprinting Processes, Annual workshop of the Franco-German Doctoral School, University of Bayreuth, 30th Sep and 1st Oct 2019 (Bayreuth, Germany)

Herbig J, Shan J, Jüngst T, Albrecht K, Groll J (2019) Microgel based bioinks, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)

Müller SJ, Bezold C, Gekle S (2019) Numerical Investigation of Cell Deformation during Bioprinting Processes, DPG-Frühjahrstagung 2019, 31 March – 5 April 2019, (Regensburg, Germany)

Müller S, Gekle S. (2018) Multiple-relaxation-time Lattice Boltzmann Implementation of Non-Newtonian Fluids, Deutsche Physikalische Gesellschaft (DPG), DPG Spring Meeting Berlin, March 11 – 16, 2018 (Berlin, Germany)

Johannes Herbig

Previous academic education: Master Funktionswerkstoffe 2016
Academic Advisor within SFB/TRR 225: Dr. Krystyna Albrecht
Location: University of Würzburg, Department for Functional Materials in Medicine and Dentistry
Topic of thesis: „Mikrofluidische Herstellung polymerer Mikrogele und Mikrokapseln für die Biofabrikation“
Main techniques and methodologies to be used: Microfluidics, Layer-by-layer coating

Contact: Johannes.herbig@fmz.uni-wuerzburg.de