B04 | 3D printing of vascular structures from vascular wall-resident stem cells

Aim of this project is the biofabrication of artificial vascular structures with correct hierarchical organization and wall morphology consisting of intima, media and adventitia. This shall be reached by the exploitation of the cellular plasticity of vascular wall-resident stem cells (VW-SCs). These cells have been shown to possess the necessary cellular plasticity to deliver such structures. By the printing of VW-SC loaded bioinks into self-healing support gels, the use of vessel wall specific matrix components and perfusion of the structures, we aim on a medium to long term to biofabricate complex and hierarchically organized vascular structures.

Prof. Dr. Süleyman Ergün
Prof. Dr. Jürgen Groll


Jürgen Groll

Ruben Gerrit Scheuring

Previous academic education: 03/2012 – 09/2015 Bachelor’s program “Molecular Biology”, Paris Lodron University Salzburg, Johannes Kepler University Linz, Austria; 09/2015 – 09/2017 Master’s program “Tissue Engineering and Regenerative  Medicine” Technikum Wien, Austria
Academic Advisor within SFB/TRR 225: Prof. Dr. Jürgen Groll
Location: University Hospital Würzburg, Abteilung für Funktionswerkstoffe der Medizin und der Zahlheilkunde
Topic of thesis: 3D Printing of Vascular Structures from Vascular Wall-Resident Stem Cells
Main techniques and methodologies to be used: Bioprinting via freeform reversible embedding, establishment of coaxial 3D bioprinting, optimization of bioinks, rheology, development of a perfusion bioreactor for tissue maturation, stem cell isolation, expansion and differentiation, cell culture, viability and functionality assays, molecular biological investigations, microscopy (light, laser, confocal and electron), in vivo tests (CAM-assay)

Contact: ruben.scheuring@fmz.uni-wuerzburg.de

Dr. Leyla Doğan

Previous academic education:
Ege University, İzmir, TR; Post Doc. Faculty of Science, Department of Biochemistry, Project Topic: Enhancement of Gene Transfection Efficiency by Functionalization of Quantum Dot-Plasmid DNA Conjugate with YopM Protein
Izmir Institute of Technology, İzmir, TR; PhD. Faculty of Science, Department of Chemistry, Thesis topic: Synthesis and Control of Exciton Dynamics in CdTe, CdTe/CdS and ZnxCd1xTe Colloidal Nanocrystals
Izmir Institute of Technology, İzmir, TR; MSc. Faculty of Science, Department of Chemistry, Thesis topic: Hydrothermal Synthesis and Characterization of Transition Metal (Mn and V) Oxides Containing Phosphates
Middle East Technical University, Ankara, TR; Bachelor of Science, Faculty of Science, Department of Chemistry
Academic Advisor within SFB/TRR 225: Prof. Süleyman Ergün
Location: University Hospital Würzburg, Institut für Anatomie und Zellbiologie
Topic: Post-Doc Researcher, project is related with Bioprinting of blood vessels using the stem cells, particularly vascular wall-resident stem cells (VW-SCs).
Main techniques and methodologies to be used: 3D bioprinting Techniques- Imaging Techniques, Material building, molecular imaging, Cell differentiation, Cell culture…

Contact: leyla.dogan@uni-wuerzburg.de

Dogan L, Scheuring R, Wagner N, Ueda Y, Wörsdörfer P, Groll J, Ergün S, Human iPSC-derived mesodermal progenitor cells preserve their vasculogenesis potential after extrusion and form hierarchically organized blood vessels, bioRxiv 2021.05.09.443303; doi:10.1101/2021.05.09.443303  (2021). (B04)

Horder H, Guaza Lasheras M, Grummel N, Nadernezhad A, Herbig J, Ergun S, Tessmar J, Groll J, Fabry B, Bauer-Kreisel P, Blunk T, Bioprinting and Differentiation of Adipose-Derived Stromal Cell Spheroids for a 3D Breast Cancer-Adipose Tissue Model, Cells 10(4) (2021) 803. (C02, A02, B02, B04)

Sun W, Starly B, Daly AC, Burdick JA, Groll J, Skeldon G, Shu W, Sakai Y, Shinohara M, Nishikawa M, Jang J, Cho DW, Nie M, Takeuchi S, Ostrovidov S, Khademhosseini A, Kamm RD, Mironov V, Moroni L, Ozbolat IT, The bioprinting roadmap, Biofabrication 12(2) (2020) 022002. (B02, B04)

Nadernezhad A, Forster L, Netti F, Adler-Abramovich L, Tessmar J, Groll J, Rheological analysis of the interplay between the molecular weight and concentration of hyaluronic acid in formulations of supramolecular HA/FmocFF hybrid hydrogels, Polym. J. 52(8) (2020) 1007-1012. (A02, B04)

Liebscher J, Tessmar J, Groll J, In Situ Polymer Analogue Generation of Azlactone Functions at Poly(oxazoline)s for Peptide Conjugation, Macromol. Chem. Phys. 221(1) (2020) 1900500. (A06, B04)

Levato R, Jungst T, Scheuring RG, Blunk T, Groll J, Malda J, From Shape to Function: The Next Step in Bioprinting, Adv. Mater. 32(12) (2020) 1906423. (A02, B04, Z01)

Berberich O, Blöhbaum J, Hölscher-Doht S, Meffert RH, Teßmar J, Blunk T, Groll J, Catechol-modified poly(oxazoline)s with tunable degradability facilitate cell invasion and lateral cartilage integration, J. Ind. Eng. Chem. 80 (2019) 757-769. (A06, B04)

Groll J, Burdick JA, Cho DW, Derby B, Gelinsky M, Heilshorn SC, Jungst T, Malda J, Mironov VA, Nakayama K, Ovsianikov A, Sun W, Takeuchi S, Yoo JJ, Woodfield TBF, A definition of bioinks and their distinction from biomaterial inks, Biofabrication 11(1) (2018) 013001. (A06, B02, B04)

Groll J (2019) 3D Printable Hydrogel-Bioinks: Rheological Implications and the Importance of Controlled Network Formation, TERMIS European Chapter Meeting 2019 27th-31st May 2019, Rhodes, Greece Tissue Engineering Therapies: From Concept to Clinical Translation & Commercialisation

Groll J (2019) Biofabrication – Chances, Challenges and Limits, Euro BioMAT 2019, 08 – 09 May 2019 (Jena-Weimar, Germany)

Groll J (2018) Biofabrication research at FMZ: from solution electrospun meshes to thiol-ene cross-linked hydrogel-bioinks, International Conference on Additive Manufacturing and Biomanufacturing,  2-4 December 2018 (Beijing, China)

Groll J (2018) Bio-ink development for Biofabrication, International Conference on Emerging Healthcare Materials, 15-16 November 2018 (Seoul, South-Korea)

Groll J (2018) SFB/TRR 225: From the Fundamentals of Biofabrication to Functional Tissue Models, International Conference on Biofabrication (ISBF) 2018, 28-31 October 2018 (Würzburg, Germany)

Groll J (2018) Hydrogels as Bioinks for Biofabrication, MSE conference, 26 – 28 September 2018, (Darmstadt, Germany)

Groll J (2018) Melt Electro Writing at the Department of Functional Materials in Medicine and Dentistry, Annual Meeting of the European Society for Biomaterials,  10 – 13 September2018 (Maastricht, Netherlands)

Groll J (2018) Biofabrication: Status of the field and translational perspective, 3D-BioNET-working-event,  18 July 2018 (Freiburg im Breisgau, Germany)

Groll J (2018) Biotinten: 3D-Druckbare Hydrogele als Schlüssel zum BioPrinting, Materialinnovationen 2018 – Vom Material zur Innovation, Konferenz zur Materialforschung des BMBF, 4. – 6. June 2018, (München, Germany)

Groll J (2018) Hydrogels as “Bio-inks” for Biofabrication, 2nd Theodore von Kármán – Discussion Conference on Materials for Life: Bioinspired and Biomimetic Hydrogels, 15 – 18 April 2018, (Bensberg, Germany).

Groll J (2018) Bioinks: Concepts, Rheological Considerations and Recent Trends, TERMIS workshop on 3D Printing in Musculoskeletal Tissue Engineering: Bioprinting in Action, 23 March 2018 (Warsaw, Poland)

Groll J (2018) 3D-Druckbare Hydrogele als Tinten für die Biofabrikation, Thüringer Biomaterial Kolloquium, 13. – 15. March 2018 (Zeulenroda, Germany)

Scheuring RG, Dogan L, Groll J, Ergün S (2019) 3D Printing of vascular wall-resident stem cells into tubular structures, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)