A07 | Fiber-reinforced hydrogels

The subproject deals with the bilateral task of improved shape fidelity and cell viability in biofabrication by applying fiber-reinforced hydrogels as bioinks. Thereby, fiber fragments which can be used both, as cell carriers and for matrix-reinforcement, are produced by electrospinning. Strikingly, the morphology of the fibers plays a significant role for cell behavior (adhesion, proliferation, alignment) as well as for the rheological and mechanical properties of the composite (flow behavior, spreading kinetics, strength), which will be investigated analytically within the project. The obtained results will allow for a systematic improvement of bioinks, which can furthermore be transferred to other materials.

Prof. Dr. Gregor Lang
Prof. Dr. Dirk Schubert

David Sonnleitner

Previous academic education: Diploma Thesis
Academic Advisor within SFB/TRR 225: Prof. Dr. Gregor Lang
Location: University of Bayreuth, Department of Engineering, Biopolymerprocessing
Topic of thesis: Development and characterization of a new bioink-system based on fiber reinforced hydrogels.
Main techniques and methodologies to be used: Bioprinting, Electrospinning, Rheology Measurements, Tensile testing

Contact: David.Sonnleitner@uni-bayreuth.de

Stefan Schrüfer

Previous academic education: Master thesis
Academic Advisor within SFB/TRR 225: Prof. Dr. Dirk W. Schubert
Location: Friedrich-Alexander Universität Erlangen-Nürnberg, Lehrstuhl für Polymerwerkstoffe – LSP
Topic of thesis: Conductive PMMA/Silver Nanowire composites
Main techniques and methodologies to be used: Meltspinning, Micro-CT,  conductivity measurement, Mechanical and rheological material tests

Contact: stefan.schruefer@fau.de

Macromolecular Journals – Best Poster Award for Stefan Schrüfer at the 16th biennial Bayreuth Polymer Symposium 2019 – 22.-24.09.2019

Best Poster Award at the 56th Nordic Polymer Days in Trondheim, Norway – 05.06.-07.06.2019

Müller SJ, Mirzahossein E, Iftekhar EN, Bächer C, Schrüfer S, Schubert DW, Fabry B, Gekle S (2020) Flow and hydrodynamic shear stress inside a printing needle during biofabrication. PLoS ONE 15(7): e0236371.

Schubert DW (2020) Novel theoretical self-consistent mean-field approach to describe the conductivity of carbon fiber filled thermoplastics—PART III—Application of the concept to mechanical properties of composites and polymer solutions. Adv. Eng. Mater. 2020

Schrüfer S, Sonnleitner D, Lang G, Schubert, DW (2020) A Novel Simple Approach to Material Parameters from Commonly Accessible Rheometer Data. Polymers, 12(6), 1276.

Hazur J, Detsch R, Karakaya E, Kaschta J, Tessmar J, Schneidereit D, Friedrich O, Schubert D, Boccaccini AR (2020) Improving alginate printability for biofabrication: establishment of a universal and homogeneous pre-crosslinking technique, Biofabrication in press

Mirzaei Z, Kuth S, Schubert DW, Detsch R, Roether J, Blunk T, Kordestani SS, Boccaccini AR (2020) Preparation and Characterization of Electrospun Blend Fibrous Polyethylene Oxide:Polycaprolactone Scaffolds to Promote Cartilage Regeneration, Advanced Engineering Materials.

Dranseikiene, D, Schrüfer, S, Schubert, DW, Reakasame S, Boccaccini AR (2020) Cell-laden alginate dialdehyde–gelatin hydrogels formed in 3D printed sacrificial gel, J Mater Sci: Mater Med 31, 31 (2020).

Tolksdorf J, Horch RE, Grüner JS, Schmid R, Kengelbach-Weigand A, Schubert DW, Werner S, Schneidereit D, Friedrich O, Ludolph I (2020) Size matters-in vitro behaviour of human fibroblasts on textured silicone surfaces with different pore sizes, J Mater Sci Mater Med. 31(2):23. 

Rausch M, Böhringer D, Steinmann M, Schubert DW, Schrüfer S, Mark C, Fabry B (2019) Measurement of skeletal muscle fiber contractility with high-speed traction microscopy. bioRxiv (2019): 733451. 

Schubert DW (2019) Revealing Novel Power Laws and Quantization in Electrospinning Considering Jet Splitting—Toward Predicting Fiber Diameter and Its Distribution. Macromolecular Theory and Simulations (2019): 1900006.

Steiner D, Lang G, Fischer L, Winkler S, Fey T, Greil P, Scheibel T, Horch RE, Arkudas A (2019) Intrinsic Vascularization of Recombinant eADF4(C16) Spider Silkmatrices in the AV Loop Model,  Tissue Engineering Part A.

Stafiej P, Küng F, Kruse FE, Schubert DW, Fuchsluger TA (2018) Mechanical and Optical Properties of PCL Nanofiber Reinforced Alginate Hydrogels for Application in Corneal Wound Healing, A SciTechnol Journal, Biomater Med Appl 2018, 2:2, DOI: 10.4172/2577-0268.1000118

Lang G, Kumari S, DeSimone E, Trossmann V, Hudel M, Spengler C, Krämer N,Jacobs K, Scheibel T (2020) Recombinant Spider Silk Films and Hydrogels with intrinsic Bacteriostatic and Fungistatic Properties, 5th Bioinspired Materials 2020 Conference, 16 – 19 March 2020 – Web conference

Lang G, Kumari S, DeSimone E, Trossmann V, Hudel M, Spengler C, Krämer N,Jacobs K, Scheibel T (2020) Bio-selective bacteriostatic and fungistatic surfaces made of recombinant spider silk proteins, 8th International Conference on Nanoscience and Nanotechnology (ICONN 2020), 9 – 13 Febrauary 2020, Brisbane, Australia

Lang G, Kumari S, Desimone E, Spengler C, Lücker S, Hudel M, Jacobs K, Krämer N, Scheibel T (2019) Bio-selective bacteriostatic and fungistatic surfaces made of recombinant spider silk proteins, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)

Schrüfer S (2019) Rheology of alginates – some new insights, Scientific Seminar of the North Bavarian Biomaterials Alliance (NBBA), 21 February 2019 (Erlangen, Germany)

Schrüfer S (2019) Rheology on alginates, DGM Regionalforum Erlangen, „Metallische Werkstoffe für die Mobilität“ 4 February 2019, (Erlangen, Germany)

Lang G (2019) Bio-selective bacteriostatic and fungistatic surfaces made of recombinant spider silk proteins, 9th biennial Australian Colloid and Interface Symposium (ACIS) 3 – 7 February 2019 (Hobart, Tasmania, Australia)

Sonnleitner D (2019) Poly-ε-caprolactone (PCL) particle – hydrogel colloid system for enhanced cell interaction, 9th biennial Australian Colloid and Interface Symposium (ACIS) 3 – 7 February 2019 (Hobart, Tasmania, Australia)

Schrüfer S, Schubert DW (2019) Revealing critical model parameters for predicting the bioprintability of hydrogel systems, 16th biennial Bayreuth Polymer Symposium 2019 – 22.-24.09.2019 (Bayreuth, Germany)

Schrüfer S, Schubert DW (2019) Revealing critical model parameters for predicting the bioprintability of hydrogel systems, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)

Allen V, Gunselmann U, Schubert DW (2019) Revealing novel power laws and quantization in electrospinning considering jet splitting – Experimental, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)

Schrüfer S, Schubert DW (2019)  Revealing critical model parameters for predicting the bioprintability of hydrogel systems, Nordic Polymer Days 2019 5 – 7 June 2019 (Trondheim, Norway)

Schrüfer S, Schubert DW (2019) Shear rheology of hydrogels – some new insights, 6th international symposium Interface Biology of Implants – IBI2019, 8 – 10 May 2019 (Warnemünde, Germany)

Schrüfer S, Himmler M, Schubert DW (2018) Shear rheology of nanofiber reinforced alginate solutions for bioprinting – establishing a basis , Jahrestagung der DGBM in Braunschweig 8-10 November 2018 (Braunschweig, Germany)

Florian Küng

Previous academic education: Ph.D. studies on “Development of a suture-able wound-cover based on aligned nano-fibers for corneal application”
Academic Advisor within SFB/TRR 225: Prof. Dr. Dirk W. Schubert
Location: Friedrich-Alexander-Universität Erlangen-Nürnberg, Lehrstuhl für Polymerwerkstoffe – LSP
Topic of thesis: Development of a suture-able wound-cover based on aligned nano-fibers for corneal application
Main techniques and methodologies to be used: Electrospinning, mechanical and rheological material tests