A06 | Cell-loaded microgels as mechanical protection and controlled microenvironment for cells in bioinks

Aim of this project is the generation of cell-loaded microgels with uniform size and their establishment as additive for bioinks. The microgels shall on the one side act as mechanical protection from shear-forces during printing and thereby extend the biofabrication window towards bioinks with higher viscosity and towards higher shear forces. In addition to that, biochemical functionalization and adjusting microgel degradability shall be used to tailor the microenvironment of the encapsulated cells and control their behavior. Prospectively, this project aims at establishing suspensions of cell-loaded microgels as bioink especially for the simultaneous printing of different cell types.

Prof. Dr. Stephan Förster
Prof. Dr. Jürgen Groll


Ilona Paulus

Previous academic education: Master of Science in chemistry
Academic Advisor within SFB TRR225: Prof. Dr. Jürgen Groll
Location: University of Würzburg, Department for functional materials in medicine and dentistry
Topic of thesis: Multifunctional POx-based building blocks for bioinks
Main techniques and methodologies to be used: Chemical synthesis, Microfluidic, 3D-Printing

Contact: ilona.paulus@fmz.uni-wuerzburg.de

Benjamin Reineke

Previous academic education: Polymer Science M.Sc. @ Uni Bayreuth
Academic Advisor within SFB TRR225: Prof. Dr. Stephan Förster
Location: Forschungszentrum Jülich
Topic of thesis: Microfluidic chips for the encapsulation of cells
Main techniques and methodologies to be used: Microfluidic chip design, soft lithography, preparation of colloids using microfluidics

Contact: b.reineke@fz-juelich.de

Dr. Stephan Hauschild

Location: Forschungszentrum Jülich, JCNS-1/ICS-1: Neutron Scattering and Soft Matter
Contact: s.hauschild@fz-juelich.de

Groll J (2019) 3D Printable Hydrogel-Bioinks: Rheological Implications and the Importance of Controlled Network Formation, TERMIS European Chapter Meeting 2019 27th-31st May 2019, Rhodes, Greece Tissue Engineering Therapies: From Concept to Clinical Translation & Commercialisation

Groll J (2019) Biofabrication – Chances, Challenges and Limits, Euro BioMAT 2019, 08 – 09 May 2019 (Jena-Weimar, Germany)

Groll J (2018) Biofabrication research at FMZ: from solution electrospun meshes to thiol-ene cross-linked hydrogel-bioinks, International Conference on Additive Manufacturing and Biomanufacturing,  2-4 December 2018 (Beijing, China)

Groll J (2018) Bio-ink development for Biofabrication, International Conference on Emerging Healthcare Materials, 15-16 November 2018 (Seoul, South-Korea)

Groll J (2018) SFB/TRR 225: From the Fundamentals of Biofabrication to Functional Tissue Models, International Conference on Biofabrication (ISBF) 2018, 28-31 October 2018 (Würzburg, Germany)

Groll J (2018) Hydrogels as Bioinks for Biofabrication, MSE conference, 26 – 28 September 2018, (Darmstadt, Germany)

Groll J (2018) Melt Electro Writing at the Department of Functional Materials in Medicine and Dentistry, Annual Meeting of the European Society for Biomaterials,  10 – 13 September2018 (Maastricht, Netherlands)

Groll J (2018) Biofabrication: Status of the field and translational perspective, 3D-BioNET-working-event,  18 July 2018 (Freiburg im Breisgau, Germany)

Groll J (2018) Biotinten: 3D-Druckbare Hydrogele als Schlüssel zum BioPrinting, Materialinnovationen 2018 – Vom Material zur Innovation, Konferenz zur Materialforschung des BMBF, 4. – 6. June 2018, (München, Germany)

Hauptstein J, Böck T, Bartolf-Kopp M, Forster L, Stahlhut P, Nadernezhad A, Blahetek G, Zernecke-Madsen A, Detsch R, Jüngst T, Groll J, Teßmar J, Blunk T (2020) Hyaluronic Acid-based Bioink Composition Enabling 3D Bioprinting and Improving Quality of Deposited Cartilaginous Extracellular Matrix, Materials Science and Engineering (MSE), 22 – 25 September 2020 – Web conference

Groll J (2018) Hydrogels as “Bio-inks” for Biofabrication, 2nd Theodore von Kármán – Discussion Conference on Materials for Life: Bioinspired and Biomimetic Hydrogels, 15 – 18 April 2018, (Bensberg, Germany).

Groll J (2018) Bioinks: Concepts, Rheological Considerations and Recent Trends, TERMIS workshop on 3D Printing in Musculoskeletal Tissue Engineering: Bioprinting in Action, 23 March 2018 (Warsaw, Poland)

Groll J (2018) 3D-Druckbare Hydrogele als Tinten für die Biofabrikation, Thüringer Biomaterial Kolloquium, 13. – 15. March 2018 (Zeulenroda, Germany)

Paulus I, Reineke B, Hauschild S, Förster S, Groll J (2020) Cell-loaded Microgels as mechanical Protection and controlled Microenvironment for Cells in Bioinks, 5th Bioinspired Materials 2020 Conference, 16 – 19 March 2020 – Web conference

Paulus I, Bloehbaum J, Tessmar J, Groll J (2019) Visible Light Cross-linked Poly(2-oxazoline) (POx)-based Hydrogels via Thiol-Ene Reaction, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)

Herbig J, Shan J, Jüngst T, Albrecht K, Groll J (2019) Microgel based bioinks, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)

Reineke B, Hauschild S, Förster S (2018) Development of microfluidic devices for the encapsulation of cells, Jülich Soft Matter Days 2018 20-23 November 2018 (Jülich, Germany)