3D printable constructs for cartilage biofabrication combining sufficient construct stability with a cell-supportive environment still represent a challenge in the field. Here, hyaluronic acid-based bioink compositions are used to modulate biological properties of 3D constructs after bioprinting and chondrogenic differentiation of MSCs, focusing on ECM distribution. The study contributes to effective bioink development and presents a perspective for cartilage regeneration.