C03 | Analysis of tumor dormancy and progression in biofabricated and in vivo vascularized 3D models

Using biofabrication methods a 3D tumor model will be developed, serving for the investigation of different aspects of tumor progression in a controlled manner both in vitro and in the vascularized in vivo AV loop model. In particular, with the establishment of a such highly-defined biofabricated melanoma tumor model essential tumor properties, such as tumor dormancy and metastasis, but also the influence of the tumor microenvironment can be analyzed and specifically modified and controlled.

Prof. Dr. Andreas Arkudas
Prof. Dr. Anja Boßerhoff
PD Dr. Annika Kengelbach-Weigand

Sonja Schmidt

Previous academic education: Master of Science
Academic Advisor within SFB TRR225: Prof. Dr. Anja Boßerhoff
Location: FAU Erlangen-Nürnberg, Department for Biochemistry and Molecular Medicine
Topic of thesis: Analysis of tumor dormancy and progression in biofabricated and in vivo vascularized 3D models
Main techniques and methodologies to be used: Analysis of cell behavior & signaling pathways (cell culture, fluorescence microscopy, qPCR, Western Blot, …)

Contact: sonja.s.schmidt@fau.de

Rafael Schmid

Previous academic education: Master of Tissue Engineering and Regenerative Medicine
Academic Advisor within SFB TRR225: Prof. Dr. Andreas Arkudas, Dr. Annika Kengelbach-Weigand
Location: University Hospital of Erlangen, Department of Plastic and Hand Surgery
Topic of thesis: Analysis of tumor dormancy and progression in biofabricated and vascularized in vivo 3D models
Main techniques and methodologies to be used: cell culture, cell culture assays, (live cell) microscopy, in vivo AV loop, in vivo 3D imaging (immuno)histology, gene expression

Contact: rafael.schmid@uk-erlangen.de

Winkler S, Mutschall H, Biggemann J, Fey T, Greil P, Korner C, Weisbach V, Meyer-Lindenberg A, Arkudas A, Horch RE, Steiner D, Human Umbilical Vein Endothelial Cell Support Bone Formation of Adipose-Derived Stem Cell-Loaded and 3D-Printed Osteogenic Matrices in the Arteriovenous Loop Model, Tissue Eng Part A 27(5-6) (2021) 413-423. (C04, C03)

Fuentes-Chandia M, Vierling A, Kappelmann-Fenzl M, Monavari M, Letort G, Hone L, Parma B, Antara SK, Ertekin O, Palmisano R, Dong M, Bopple K, Boccaccini AR, Ceppi P, Bosserhoff AK, Leal-Egana A, 3D Spheroids Versus 3D Tumor-Like Microcapsules: Confinement and Mechanical Stress May Lead to the Expression of Malignant Responses in Cancer Cells, Adv. Biol. doi:10.1002/adbi.202000349  (2021) 2000349. (C03, A01)

Tolksdorf J, Horch RE, Gruner JS, Schmid R, Kengelbach-Weigand A, Schubert DW, Werner S, Schneidereit D, Friedrich O, Ludolph I, Size matters-in vitro behaviour of human fibroblasts on textured silicone surfaces with different pore sizes, J. Mater. Sci.: Mater. Med. 31(2) (2020) 23. (C04, B08, A07, C03)

Schmid R, Schmidt SK, Hazur J, Detsch R, Maurer E, Boccaccini AR, Hauptstein J, Tessmar J, Blunk T, Schrufer S, Schubert DW, Horch RE, Bosserhoff AK, Arkudas A, Kengelbach-Weigand A, Comparison of Hydrogels for the Development of Well-Defined 3D Cancer Models of Breast Cancer and Melanoma, Cancers 12(8) (2020) 2320. (C03, A01, A02, A07, C02)

Mutschall H, Winkler S, Weisbach V, Arkudas A, Horch RE, Steiner D, Bone tissue engineering using adipose-derived stem cells and endothelial cells: Effects of the cell ratio, J. Cell. Mol. Med. 24(12) (2020) 7034-7043. (C04, C03)

Bohme I, Bosserhoff A, Extracellular acidosis triggers a senescence-like phenotype in human melanoma cells, Pigm. Cell Melanoma Res. 33(1) (2020) 41-51. (C03)

Steiner D, Lang G, Fischer L, Winkler S, Fey T, Greil P, Scheibel T, Horch RE, Arkudas A, Intrinsic Vascularization of Recombinant eADF4(C16) Spider Silk Matrices in the Arteriovenous Loop Model, Tissue Eng Part A 25(21-22) (2019) 1504-1513. (C04, A07, C01, C03)

Schmidt SK, Schmid R, Arkudas A, Kengelbach-Weigand A, Bosserhoff AK, Tumor Cells Develop Defined Cellular Phenotypes After 3D-Bioprinting in Different Bioinks, Cells 8(10) (2019) 1295. (C03)

Kengelbach-Weigand A, Tasbihi K, Strissel PL, Schmid R, Marques JM, Beier JP, Beckmann MW, Strick R, Horch RE, Boos AM, Plasticity of patient-matched normal mammary epithelial cells is dependent on autologous adipose-derived stem cells, Sci. Rep. 9(1) (2019) 10722. (C03)

Weigand A, Horch RE, Boos AM, Beier JP, Arkudas A, The Arteriovenous Loop: Engineering of Axially Vascularized Tissue, Eur. Surg. Res. 59(3-4) (2018) 286-299. (C03, C04)

Steiner D, Lingens L, Fischer L, Kohn K, Detsch R, Boccaccini AR, Fey T, Greil P, Weis C, Beier JP, Horch RE, Arkudas A, Encapsulation of Mesenchymal Stem Cells Improves Vascularization of Alginate-Based Scaffolds, Tissue Eng Part A 24(17-18) (2018) 1320-1331. (C03, C04, A01, B06, B03) Front Cover for Tissue Engineering Part A

Rottensteiner-Brandl U, Detsch R, Sarker B, Lingens L, Kohn K, Kneser U, Bosserhoff AK, Horch RE, Boccaccini AR, Arkudas A, Encapsulation of Rat Bone Marrow Derived Mesenchymal Stem Cells in Alginate Dialdehyde/Gelatin Microbeads with and without Nanoscaled Bioactive Glass for In Vivo Bone Tissue Engineering, Materials (Basel) 11(10) (2018) 1880. (C04, A01, C03)

An R, Schmid R, Klausing A, Robering JW, Weber M, Bauerle T, Detsch R, Boccaccini AR, Horch RE, Boos AM, Weigand A, Proangiogenic effects of tumor cells on endothelial progenitor cells vary with tumor type in an in vitro and in vivo rat model, FASEB J. 32(10) (2018) 5587-5601. (C03)

Schmid R, Schmidt S, Hazur J, Detsch R, Boccaccini AR, Bosserhoff A, Horch RE, Arkudas A, Kengelbach-Weigand A (2019) Analyse von Tumor Dormancy und Progression in biofabrizierten und in vivo vaskularisierten 3D Modellen; 50. Jahrestagung der Deutschen Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen (DGPRÄC) September 26-28, 2019 (Hamburg, Germany)

Kengelbach-Weigand A (2019) In vitro und in vivo Modelle zur Erforschung der Mammakarzinompathogenese; 50. Jahrestagung der Deutschen Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen (DGPRÄC) September 26-28, 2019 (Hamburg, Germany)

Kengelbach-Weigand A, Schmid R, Tasbihi K, Strissel PL, Strick R, Al-Abboodi M, An R, Robering JW, Eckstein M, Bäuerle T, Detsch R, Boccaccini AR, Dalton PD, Youssef A, Wolf K, Kremer AE, Boos AM, Bosserhoff A, Arkudas A, Horch RE (2019) The arteriovenous loop as 3D model for studying the interaction of ADSC and breast cancer cells, TERMIS EU Workshop 2019, 26th-27th Aug 2019, Nantes, France

Kengelbach-Weigand A (2019) Das arteriovenöse Gefäßschleifenmodell als Tool für die Untersuchung der Interaktion von ADSC und Mammakarzinomzellen, 3. DAM Wissenschaftsakademie, 12 – 13 April 2019 (Erlangen, Germany)

Schmid R (2019) Analyse von Tumor dormancy und Progression in biofabrizierten und in vivo vaskularisierten 3D-Modellen, 3. DAM Wissenschaftsakademie, 12 – 13 April 2019 (Erlangen, Germany)

Weigand A, Boos AM, An R, Strissel PL, Schmid R, Robering JW, Al-Abboodi M, Detsch R, Dalton PD, Youssef A , Boccaccini AR, Beckmann MW, Bäuerle T, Strick R, Horch RE (2018) Vom Tissue Engineering zum Tumor Engineering, 49. Jahrestagung der Deutsche Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen DGPRÄC & 23. Jahrestagung der Vereinigung der Deutschen Ästhetisch Plastischen Chirurgen (VDÄPC), 13 – 15 September 2018 (Bochum, Germany)

Weigand A, Beier JP, Schmid R, Robering JW, Cai A, Arkudas A, Horch RE, Boos A (2018) Vascularized Tissue Engineering Using the Arteriovenous Loop – The Path from the Small Animal Model to the Preclinical Large Animal Model, 2018 TERMIS World Congress, 4-7 September 2018 (Kyoto, Japan)

Peddi A, Steiner D, Weigand A, Lingens L, Fischer L, Köhn K, Detsch R, Boccaccini AR, Fey T, Greil P, Weis C, Beier JP, Horch RE, Arkudas A. (2018) Encapsulation of mesenchymal stem cells improves vascularization of alginate-based scaffolds,  The 10th EPSRC Meeting, 23 – 25 August 2018 (Hamburg, Germany)

Weigand A, Boos AM, An R, Strissel PL, Schmid R, Robering JW, Al-Abboodi M, Detsch R, Dalton PD, Youssef A , Boccaccini AR, Beckmann MW, Bäuerle T, Strick R, Horch RE (2018) From Tissue Engineering to Tumor Engineering –  Application of the arteriovenous loop model for the generation of a fully vascularized tumor in an animal model, 2018 TERMIS World Congress, 4-7 September 2018 (Kyoto, Japan)

Ajay Peddi

Previous academic education: Master in Biomedical Engineering, Martin Luther University Halle, Germany Effects of Fresh Blood Versus Old Blood (stored) on Hepatocyte Viability, Proliferation, Gene Expression; Comparison of the Effects in Hepatocyte 2D and 3D Cell Culture Models
Bachelor of Pharmacy, Rajeev Gandhi University of Health Sciences, India
Academic Advisor within SFB TRR225: Prof. Dr. Andreas Arkudas, Dr. Annika Weigand
Location: University Hospital of Erlangen, Department of Plastic and Hand Surgery
Topic of thesis: Analysis of tumor dormancy and progression in biofabricated and vascularized in vivo 3D models
Main techniques and methodologies to be used: cell culture, cell culture assays, (live cell) microscopy, in vivo AV loop, in vivo 3D imaging (immuno)histology, gene expression