C02 | Biofabrication of a 3D model for the functional investigation of the stromal parameters on the behavior of breast cancer cells

Project C02 aims at the biofabrication of a complex tumor-stroma model to specifically study the behavior of breast cancer cells as a function of stromal parameters. To achieve this goal, innovative 3D printing technologies will be applied to combine breast cancer and stromal cells in bioink matrices with independently controllable physicochemical features. This modular approach allows us to recapitulate relevant aspects of the tumor microenvironment and to gain important mechanistic insights into how tumor-stroma interactions contribute to tumorigenesis.

Prof. Dr. Ben Fabry
Prof. Dr. Torsten Blunk

Prof. Dr. Torsten Blunk

University Hospital Würzburg, Department of Trauma, Hand, Plastic and Reconstructive Surgery
Contact: torsten.blunk@fmz.uni-wuerzburg.de

Prof. Dr. Ben Fabry

Friedrich-Alexander University of Erlangen-Nuremberg, Physics department
Contact: bfabry@biomed.uni-erlangen.de

Nadine Endrizzi (Grummel)

Previous academic education: M.Sc. Cell- and Molecularbiology
Academic Advisor within SFB TRR225: Prof. Dr. Ben Fabry
Location: University of Erlangen-Nürnberg, Department for Biophysics
Topic of thesis: Biofabrication of a 3D model for functional analysis of stromal determinants on breast cancer cells‘ behavior
Main techniques and methodologies to be used: Cell culture, confocal microscopy

Contact: nadine.endrizzi@fau.de

Hannes Horder

Previous academic education: Molecular Biotechnology (M. Sc., Goethe-University Frankfurt), Applied Bio-, Pharma- and Medical Sciences (B. Sc., University of Applied Sciences Kaiserslautern)
Academic Advisor within SFB TRR225: Prof. Dr. Torsten Blunk
Location: University Hospital Würzburg, Department of Trauma, Hand, Plastic and Reconstructive Surgery
Topic of thesis: Biofabrication of a 3D Model for the Functional Investigation of the Impact of Stromal Determinants on the Behavior of Breast Cancer Cells
Main techniques and methodologies to be used: 2D/3D Cell Culture, Histology, Immunohistochemistry, Microscopy, quantitative DNA- and ECM-Assays, FACS, 3D Bioprinting

Contact: Horder_H@ukw.de

Spörrer M, Kah D, Gerum RC, Reischl B, Huraskin D, Dessalles CA, Schneider W, Goldmann WH, Herrmann H, Thievessen I, Clemen CS, Friedrich O, Hashemolhosseini S, Schröder R, Fabry B, doi:10.1101/2021.07.23.453481 (2021). (A01, B08, C02)

Kah D, Winterl A, Přechová M, Schöler U, Schneider W, Friedrich O, Gregor M, Fabry B, A low-cost uniaxial cell stretcher for six parallel wells, HardwareX 9 (2021). (A01, B08, C02)

Horder H, Guaza Lasheras M, Grummel N, Nadernezhad A, Herbig J, Ergun S, Tessmar J, Groll J, Fabry B, Bauer-Kreisel P, Blunk T, Bioprinting and Differentiation of Adipose-Derived Stromal Cell Spheroids for a 3D Breast Cancer-Adipose Tissue Model, Cells 10(4) (2021) 803. (C02, A02, B02, B04)

Hazur J, Endrizzi N, Schubert DW, Boccaccini AR, Fabry B, Stress relaxation amplitude of hydrogels determines migration, proliferation, and morphology of cells in 3-D, bioRxiv (2021) 2021.07.08.451608 (A01, A07, C02)

Schmid R, Schmidt SK, Hazur J, Detsch R, Maurer E, Boccaccini AR, Hauptstein J, Tessmar J, Blunk T, Schrufer S, Schubert DW, Horch RE, Bosserhoff AK, Arkudas A, Kengelbach-Weigand A, Comparison of Hydrogels for the Development of Well-Defined 3D Cancer Models of Breast Cancer and Melanoma, Cancers 12(8) (2020) 2320. (C03, A01, A02, A07, C02)

Reimann R, Kah D, Mark C, Dettmer J, Reimann TM, Gerum RC, Geitmann A, Fabry B, Dietrich P, Kost B, Durotropic Growth of Pollen Tubes, Plant Physiol. 183(2) (2020) 558-569. (A01, C02)

Mark C, Grundy TJ, Strissel PL, Bohringer D, Grummel N, Gerum R, Steinwachs J, Hack CC, Beckmann MW, Eckstein M, Strick R, O’Neill GM, Fabry B, Collective forces of tumor spheroids in three-dimensional biopolymer networks, eLife 9 (2020) 51912. (C02, B01)

Hoefner C, Muhr C, Horder H, Wiesner M, Wittmann K, Lukaszyk D, Radeloff K, Winnefeld M, Becker M, Blunk T, Bauer-Kreisel P, Human Adipose-Derived Mesenchymal Stromal/Stem Cell Spheroids Possess High Adipogenic Capacity and Acquire an Adipose Tissue-like Extracellular Matrix Pattern, Tissue Eng Part A 26(15-16) (2020) 915-926. (C02)

Bachmann J, Ehlert E, Becker M, Otto C, Radeloff K, Blunk T, Bauer-Kreisel P, Ischemia-Like Stress Conditions Stimulate Trophic Activities of Adipose-Derived Stromal/Stem Cells, Cells 9(9) (2020) 1935. (C02)

McMaster R, Hoefner C, Hrynevich A, Blum C, Wiesner M, Wittmann K, Dargaville TR, Bauer-Kreisel P, Groll J, Dalton PD, Blunk T, Tailored Melt Electrowritten Scaffolds for the Generation of Sheet-Like Tissue Constructs from Multicellular Spheroids, Adv Healthc Mater 8(7) (2019) 1801326. (C02, A04)

Condor M, Mark C, Gerum RC, Grummel NC, Bauer A, Garcia-Aznar JM, Fabry B, Breast Cancer Cells Adapt Contractile Forces to Overcome Steric Hindrance, Biophys. J. 116(7) (2019) 1305-1312. (C02)

 

Horder H, Lasheras MG, Grummel N, Fabry B, Teßmar J, Bauer-Kreisel P, Blunk T (2020) 3D Bioprinting of Multicellular Adipose-derived Stromal Cell Spheroids in Hyaluronic Acid-based Bioinks, 5th Bioinspired Materials 2020 Conference, 16 – 19 March 2020 – Web conference

Grummel N, Mark C, Böhringer D, Horder H, Strissel P, Strick R , Blunk T, Fabry B (2019) Invasion, migration and force generation of primary epithelial and mesenchymal breast cancer cells, 10th Physics of Cancer –  25-27 September 2019 (Leipzig, Germany)

Fischer L, Karakaya E, Distler T, Grummel N, Boccaccini AR, Fabry B, Detsch R, Thievessen I (2018) Shear stress and cellular behaviour in 3 D biofabrication assays, 9th Annual Symposium “Physics of Cancer” 24-26 September 2018 (Leipzig, Germany)