C01 | Biofabrication of heart substitute tissue based on bioink from spider silk proteins

Heart diseases are a major socioeconomic burden. Despite considerable progress in the prevention and minimization of heart damage, the prevalence of heart insufficiency increases continuously. The aim of this subproject is the reproducible production of human cardiac substitute tissue based on a scaffold with adjustable mechanical properties from spider silk bioink and cardiac cells using 3D printing technology. The long-term goal is the treatment of heart diseases.

Prof. Dr. Felix Engel
Prof. Dr. Thomas Scheibel

Tilman Esser

Previous academic education: M.Sc (Cardiovascular Science), B.Sc. (Molecular Medicine), University of Göttingen
Academic Advisor within SFB TRR225: Prof. Dr. Felix Engel
Location: Universitätsklinikum Erlangen, Nephropathology
Topic of thesis: Biofabrication of Silk-based Artificial Heart Tissue
Main techniques and methodologies to be used: Stem Cell Culture, Cardiac Differentiation, Bioprinting, Cell & Molecular Analysis

Contact: tilman.esser@uk-erlangen.de

First SFB/TRR 225 PhD student with first authorship

Vanessa Trossmann

Previous academic education: Master-thesis
Academic Advisor within SFB TRR225: Prof. Dr. Thomas Scheibel
Location: University of Bayreuth, Chair of Biomaterials
Topic of thesis: Recombinant spider silk proteins with designed cell-substrate-interaction for biomedical applications
Main techniques and methodologies to be used: Cloning, recombinant protein expression and purification, protein characterization, protein processing (films, bioinks), 3D-Bioprinting, Cell culture

Contact: vanessa.trossmann@bm.uni-bayreuth.de

Annika Döbl

Previous academic education: Master-thesis
Academic Advisor within SFB TRR225: Prof. Dr. Thomas Scheibel
Location: University of Bayreuth, Chair of Biomaterials
Topic of thesis: Spider silk-based 3D cultivation of tumor cells as in vitro model for cancer research
Main techniques and methodologies to be used: 3D printing/bioprinting, cell culture, protein processing, chemical protein modification

Contact: annika.doebl@bm.uni-bayreuth.de

Roshanbinfar K, Mohammadi Z, Sheikh-Mahdi Mesgar A, Dehghan MM, Oommen OP, Hilborn J, Engel FB (2019) Carbon nanotube doped pericardial matrix derived electroconductive biohybrid hydrogel for cardiac tissue engineering, Biomater. Sci., 2019,7, 3906-3917 

Steiner D, Lang G, Fischer L, Winkler S, Fey T, Greil P, Scheibel T, Horch RE, Arkudas A (2019) Intrinsic Vascularization of Recombinant eADF4(C16) Spider Silkmatrices in the AV Loop Model,  Tissue Engineering Part A.

Madonna R, Van Laake LW, Botker HE, Davidson SM, De Caterina R, Engel FB, Eschenhagen T, Fernandez-Aviles F, Hausenloy DJ, Hulot JS, Lecour S, Leor J, Menasché P, Pesce M, Perrino C, Prunier F, Van Linthout S, Ytrehus K, Zimmermann WH, Ferdinandy P, Sluijter JPG (2019). Position paper for Cardiovascular Research. ESC Working Group on Cellular Biology of the Heart: Tissue Engineering and Cell-Based Therapies for Cardiac Repair in Ischemic Heart Disease and Heart Failure. Cardiovasc Res. 2019 Jan 17. doi: 10.1093/cvr/cvz010. PubMed PMID: 30657875.

Esser TU, Roshanbinfar K, Engel FB (2018) Promoting vascularization for tissue engineering constructs: current strategies focusing on HIF-regulating scaffolds, Expert Opin Biol Ther. 2018 Dec 20. doi: 10.1080/14712598.2019.1561855

Roshanbinfar K, Vogt L, Greber B, Diecke S, Boccaccini A, Scheibel T, Engel FB. (2018) Electroconductive Biohybrid Hydrogel for Enhanced Maturation and Beating Properties of Engineered Cardiac Tissues, Adv Funct Mater, 2018 Sep 06. doi: 10.1002/adfm.201803951

Engel FB (2018) Funktionale Gewebemodelle: Neue Materialien für die Herzgewebeersatztherapie, connexi – das Konferenzmagazin, connexi Kardiologie, Ausgabe 8-2018 The Paideia Group GmbH

Roshanbinfar K, Vogt L, Greber B, Diecke S, Boccaccini AR, Mohammadi Z, Mesgar AS, Dehghan MM, Oommen OP, Scheibel T, Hilborn J, Engel FB (2019) Electroconductive materials enhance maturation of hiPSC-derived cardiomyocytes in engineered cardiac tissues, TERMIS European Chapter Meeting 2019 27th – 31st May 2019, Rhodes, Greece Tissue Engineering Therapies: From Concept to Clinical Translation & Commercialisation

Scheibel T (2019) Drug delivery vehicles based on engineered spider silk protein, 9th biennial Australian Colloid and Interface Symposium (ACIS) 3 – 7 February 2019 (Hobart, Tasmania, Australia)

Trossmann V (2019) Recombinant spider silk proteins for cardiac tissue engineering, 9th biennial Australian Colloid and Interface Symposium (ACIS) 3 – 7 February 2019 (Hobart, Tasmania, Australia)

Engel FB (2018) TRR 225: Biofabrikation von funktionalen Gewebemodellen, Invited speaker at 27. Jahrestagung der Deutschen Transplantationsgesellschaft (DTG 2018), 7-10 November 2018 (Berlin, Germany)

Engel FB (2018) New scaffolds for cardiac repair, Invited presentation at: International Conference on Biofabrication (ISBF) 2018, 28-31 October 2018 (Würzburg, Germany)

Roshanbinfar K (2018) Electroconductive Biohybrid Hydrogel for Enhanced Maturation and Beating Properties of Engineered Cardiac Tissues, Invited presentation at: International Conference on Biofabrication (ISBF) 2018, 28-31 October 2018 (Würzburg, Germany)

Scheibel T (2018) Biopolymer-based materials, Gesellschaft Deutscher Chemiker e.V. GDCh-Vortrag/Hamburg 25-26 October 2018 (Hamburg, Germany)

Scheibel T (2018) Was Spider-Man besser wissen sollte… Landesamt für Gesundheit 23 October 2018 (Erlangen, Germany)

Scheibel T (2018) Biofabrication with bioinks made of rekombinant spider silk, N.I.C.E. Conference 2018, The 4th International Conference on Bioinspired and Biobased Chemistry & Materials, 14-17 October 2018 (Nice, France)

Scheibel T (2018) Development of spider silk applications, Siemens Healthcare GmbH 1 October 2018 (Kemnath, Germany)

Engel FB (2018) New scaffolds for cardiac repair, ESC Congress 2018 (European Cardiac Society) 25-29 August 2018 (Munich, Germany)

Scheibel T (2018) Engineering, processing and applications of structural proteins, UPAC und Büroeröffnung 1-5 July 2018  (Melbourne Cairns, Australia)