B06 | Reporter constructs and reporter cells for the investigation of cellular stress reply and signal transduction during biofabrication

The goal of B06 is to understand which stresses cells experience during the bioprinting process and in the maturing biofabricates, and how these stresses affect cell behavior. Using lentiviral, fluorescent reporter constructs and reporter cells we will monitor various stress parameters in different cell types in response to bioplotting and inkjet printing conditions, specific integrin-ligation and matrix-stiffness in alginate based biofabricates. We will correlate the stress responses with the activities of specific integrin-dependent signaling processes in order to identify, analyze and modulate molecular mechanisms that determine the cellular response to stresses in alginate based biofabricates.

Dr. Ingo Thievessen
Dr. Rainer Detsch

Lena Fischer

Previous academic education: Master Thesis
Academic Advisor within SFB/TRR 225: Dr. Ingo Thievessen
Location: Friedrich-Alexander UniversityErlangen/Nürnberg, Biophysics group, Center for Medical Physics and Technology
Topic of thesis: Cellular stress response and integrin-mediated signaling in biofabrication processes
Main techniques and methodologies to be used: Lentiviral reporter constructs, cell culture, fluorescent live cell microscopy, bioprinting

Contact: lena.lf.fischer@fau.de

Emine Karakaya

Previous academic education: B.Sc. Chemistry: “Synthesis and Characterization of the Buckminsterfullerenes C60H36und C60H18“, M. Sc. Chemistry: “Design of Goldnanorods for Nanoplasmonics”
Academic Advisor within SFB/TRR 225: Prof. Dr.-Ing. habil. Aldo R. Boccaccini, Dr. Rainer Detsch (technical advisor)
Location: Friedrich-Alexander University of Erlangen-Nuremberg, Institute of Biomaterials
Topic of thesis: Chemical functionalization of bioinks, characterization techniques, developing of bioinks for biofabrication
Main techniques and methodologies to be used: Spectroscopic methods to analyze chemical interaction of polysaccharides and peptides, bioplotting and inkjet printing, cell culture

Contact: emine.karakaya@fau.de

Hazur J, Detsch R, Karakaya E, Kaschta J, Tessmar J, Schneidereit D, Friedrich O, Schubert D, Boccaccini AR (2020) Improving alginate printability for biofabrication: establishment of a universal and homogeneous pre-crosslinking technique, Biofabrication in press

Mirzaei Z, Kuth S, Schubert DW, Detsch R, Roether J, Blunk T, Kordestani SS, Boccaccini AR (2020) Preparation and Characterization of Electrospun Blend Fibrous Polyethylene Oxide:Polycaprolactone Scaffolds to Promote Cartilage Regeneration, Advanced Engineering Materials.

Distler T, Ruther F, Boccaccini AR, Detsch R (2019) Development of 3D Biofabricated Cell Laden Hydrogel Vessels and a Low-Cost Desktop Printed Perfusion Chamber for In Vitro Vessel Maturation, Macromol. Biosci.2019, 1900245

Ruther F, Distler T, Boccaccini AR, Detsch R (2018) Biofabrication of vessel-like structures with alginate di-aldehyde—gelatin (ADA-GEL) bioink, J Mater Sci Mater Med. 2018 Dec 29;30(1):8. doi: 10.1007/s10856-018-6205-7. January 2019, 30:8

Rottensteiner-Brandl U, Detsch R, Sarker B, Lingens L, Köhn K, Kneser U, Bosserhoff AK, Horch RE, Boccaccini AR, Arkudas A. (2018) Encapsulation of Rat Bone Marrow Derived Mesenchymal Stem Cells in Alginate Dialdehyde/Gelatin Microbeads with and without Nanoscaled Bioactive Glass for In Vivo Bone Tissue Engineering. Materials (Basel). 2018 Oct 1;11(10). pii: E1880. doi: 10.3390/ma11101880

Steiner D, Lingens L, Fischer L, Köhn K, Detsch R, Boccaccini AR, Fey T, Greil P, Weis C, Beier J, Horch RE, Arkudas A. (2018) Encapsulation of mesenchymal stem cells improves vascularization of alginate-based scaffolds. Tissue Eng Part A. 2018 Mar 20. doi: 10.1089/ten.TEA.2017.0496.      Front Cover for Tissue Engineering Part A

Detsch R (2019) Leben aus dem 3D Drucker: Chancen, Herausforderungen und Limitationen für verschiedene medizintechnische Anwendungen, Collegium Alexandrinum der Universität Erlangen-Nürnberg – Wissenschaft für die Öffentlichkeit – Novmber, 28, 2019 (Erlangen Germany)

Detsch R, Hazur J, Karakaya E, Kuth S, Heid S, Boccaccini AR (2019) Evaluating three different extrusion-based bioprinting systems using cell-laden alginate-based bioinks, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)

Detsch R (2019) From the Fundamentals of Biofabrication towards Functional Tissue Models, 1st Heidelberg Workshop on 3D-Bioprinting, 18 July 2019 (Heidelberg, Germany).

Detsch R, Boccaccini AR (2019) Challenges in biofabrication of alginate-based bioinks for different biomedical approaches, Deutsches Zentrum für Luft-und Raumfahrt in Bonn, Workshop Additive Fertigung in der Raumfahrt. 28 May 2019 (Bonn, Germany)

Detsch R, Distler T, Ruther F, Boccaccini AR (2018) Engineering of Vessel Grafts using Alginate di-aldehyde – Gelatin Bioink, International Conference on Biofabrication (ISBF) 2018, 28-31 October 2018 (Würzburg, Germany)

Detsch R, Liverani L, Boccaccini AR (2018) Biomaterials research in Erlangen: from bioactive materials to tissue scaffolds and biofabrication, UMN-FAU Workshop, 19 – 20 September 2018 (University Minnesota, USA)

Karakaya E, Frank A, Forster L, Teßmar J, Schmidt HW, Boccaccini AR, Detsch R (2020) Evaluation of inkjet printing for ADA-PEG bioinks, 5th Bioinspired Materials 2020 Conference, 16 – 19 March 2020 – Web conference

Fischer L, Karakaya E, Müller S, Schrüfer S, Distler T, Schubert DW, Gekle S, Fabry B, Boccaccini AR, Detsch R, Thievessen I (2019) Shear stress and cellular behaviour in 3 D biofabrication assays, Goodbye Flat Biology: Advancing 3D-based Models for Cancer Biology and Drug Discovery – 10.-13.11.2019 (Berlin, Germany)

Karakaya E, Hazur J, Teßmar J, Boccaccini AR, Detsch R(2019) How to Determine a Suitable Sodium Alginate for the Fabrication of Standardized Alginatedialdehyde-Gelatin crosslinked Hydrogels in Biofabrication Approaches? Annual Conference of the International Society for Biofabrication (ISBF), 20-22 October 2019 (Columbus, Ohio, USA)

Karakaya E, Teßmar J, Boccaccini AR, Detsch R (2019) Evaluation of the Biopolymer Modification for the Reproducible Production of Alginate-based Bioinks, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)

Fischer L, Karakaya E, Distler T, Boccaccini AR, Fabry B, Detsch R, Thievessen I (2018) Concept studies of the application of reporter cells in biofabrication approaches, Annual Conference of the International Society for Biofabrication (ISBF), 28-31 October 2018 (Würzburg, Germany)

Fischer L, Karakaya E, Distler T, Grummel N, Boccaccini AR, Fabry B, Detsch R, Thievessen I (2018) Shear stress and cellular behaviour in 3 D biofabrication assays, 9th Annual Symposium “Physics of Cancer” 24-26 September 2018 (Leipzig, Germany)

Detsch R, Boccaccini AR (2018) Advances in Biomaterials for Biofabrication: 3D Printing with Bioinks, “Tag der Technischen Fakultät” of the FAU Erlangen 16 November 2018 (Erlangen, Germany)

Schneidereit D, Sulistio AA, Bröllochs A, Lu K, Distler T, Schürmann S, Detsch R, Dendorfer A, Boccaccini AR, Friedrich O (2018) 3D Tissue Substructure: Using Multi-Photon Excitation to Reveal Cellular 3D Morphology of Natural and Engineered Tissue in Depth, Annual Conference of the International Society for Biofabrication (ISBF), 28-31 October 2018 (Würzburg, Germany)