B06 | Reporter constructs and reporter cells for the investigation of cellular stress reply and signal transduction during biofabrication
The goal of B06 is to understand which stresses cells experience during the bioprinting process and in the maturing biofabricates, and how these stresses affect cell behavior. Using lentiviral, fluorescent reporter constructs and reporter cells we will monitor various stress parameters in different cell types in response to bioplotting and inkjet printing conditions, specific integrin-ligation and matrix-stiffness in alginate based biofabricates. We will correlate the stress responses with the activities of specific integrin-dependent signaling processes in order to identify, analyze and modulate molecular mechanisms that determine the cellular response to stresses in alginate based biofabricates.
