B05 | Glycoengineering as a tool to control the behavior of mesenchymal stem cells in biofabrication processes

The modification of the microenvironment of cells by using click chemistry and synthetic sugar structures represents a new tool for the development of bioinks as well as for the transient modification of cell surfaces. Bioinks will be functionalized by glycoengineering in such a way that cells selectively adhere and differentiate. On the other hand, the extracellular glycocalyx will be modified and/or a protective polysaccharide layer will be added to protect cells from shear forces arising during bioprinting. The long-term goal is to modify cells and bioinks by glycoengineering in such a way that the post-production behavior of cells is optimized in biofabricated constructs.

PD Dr. Regina Ebert
Prof. Dr. Jürgen Seibel

Stephan Altmann

Previous academic education: M.Sc. Biochemistry, Topic of thesis: “To be or not to be planktonic? Investigation of single species cyanobacterial biofilms”
Academic Advisor at SFB/TRR 225: PD Dr. Regina Ebert
Location: University of Wuerzburg, Orthopedic Center for Musculoskeletal Research
Topic of thesis: Glycoengineering as a tool for regulating behaviour of mesenchymal stem cells in biofabrication
Main techniques and methodologies to be used: Cell culture techniques, Click chemistry (bioconjugation), Biochemical assays (proliferation, apoptosis), qPCR (differentiation, senescence, mechanotransduction), Microscopy (differentiation, senescence, viability, stiffness)

Contact: stephan.altmann@klh.de

Jürgen Mut

Previous academic education:  Master of Science
Academic Advisor within SFB/TRR 225: Prof. Dr. Jürgen Seibel
Location: University of Wuerzburg, Institute of Organic Chemistry
Topic of thesis: Synthese von Fluor-substituierten, aromatischen Galektin-1 Inhibitoren
Main techniques and methodologies to be used: Organic synthesis

Contact: juergen.mut@uni-wuerzburg.de

Ertl J, Ortiz-Soto ME, Anh Le T, Bechold J, Shan J, Teßmar J, Engels B, Seibel J (2019) Tuning the product spectrum of a glycoside hydrolase enzyme by a combination of site‐directed mutagenesis and tyrosine‐specific chemical modification Chem. Eur. J. 10.1002/chem.201900576

Ziouti F, Ebert R, Rummler M, Krug M, Müller-Deubert S, Lüdemann M, Jakob F, Willie BM, Jundt F (2019) NOTCH signaling is activated through mechanical strain in human bone marrow-derived mesenchymal stromal cells, Stem Cells International Volume 2019, Article ID 5150634, 13 pages

Gutmann M, Bechold J, Seibel J, Meinel L,, Lühmann T  (2018) Metabolic Glycoengineering of Cell-Derived Matrices and Cell Surfaces: A Combination of Key Principles and Step-by-Step Procedures, ACS Biomater. Sci. Eng., Article ASAP,DOI: 10.1021/acsbiomaterials.8b00865,Publication Date (Web): November 20, 2018

Gutmann M, Braun A, Seibel J, Lühmann T. (2018) Bioorthogonal Modification of Cell Derived Matrices by Metabolic Glycoengineering. ACS Biomater. Sci. Eng. 2018 Mar 13, doi: 10.1021/acsbiomaterials.8b00264.

Ortiz-Soto ME, Ertl J, Mut J, Adelmann J, Le TA, Shan J, Teßmar J, Schlosser A, Engels B, Seibel J. (2018) Product-oriented chemical surface modification of a levansucrase (SacB) via an ene-type reaction., Chem Sci. 2018 May 22;9(24):5312-5321. doi: 10.1039/c8sc01244j. eCollection 2018 Jun 28.   Front Cover Picture for Chemical Science   Press Release

Seibel, J (2018) Glycoengineering as a tool for visualizing and redesigning the human and bacterial cell surfaces, GlykoT 2018, The 11th International Symposium on Glycosyltransferases, 19-23 June 2018 (Qingdao, China)

Julian Bechold

Previous academic education: Master-Thesis
Academic Advisor within SFB/TRR 225: Prof. Dr. Jürgen Seibel
Location: University of Würzburg, Institute of Organic Chemistry
Topic of thesis: Synthesis of Galectin-1-Inhibitors
Main techniques and methodologies to be used: Organic Synthesis, Chromatography, Spectrometry, protein expression

Contact: julian.bechold@uni-wuerzburg.de

Natalia Wolf

Previous academic education:  M. Sc. Chemistry
Academic Advisor within SFB/TRR 225: Prof. Dr. Jürgen Seibel
Location: University of Wuerzburg, Institute of Organic Chemistry
Topic of thesis: Synthesis and application of cyanine based dyes in super resolution microscopy.
Main techniques and methodologies to be used: Organic synthesis, spectroscopy, modification and functionalization of various materials (glas slides, gold chips).

Contact: natalia.wolf@uni-wuerzburg.de