B05 | Glycoengineering as a tool to control the behavior of mesenchymal stem cells in biofabrication processes

The modification of the microenvironment of cells by using click chemistry and synthetic sugar structures represents a new tool for the development of bioinks as well as for the transient modification of cell surfaces. Bioinks will be functionalized by glycoengineering in such a way that cells selectively adhere and differentiate. On the other hand, the extracellular glycocalyx will be modified and/or a protective polysaccharide layer will be added to protect cells from shear forces arising during bioprinting. The long-term goal is to modify cells and bioinks by glycoengineering in such a way that the post-production behavior of cells is optimized in biofabricated constructs.

Prof. Dr. Regina Ebert
Prof. Dr. Jürgen Seibel

Stephan Altmann

Previous academic education: M.Sc. Biochemistry, Topic of thesis: “To be or not to be planktonic? Investigation of single species cyanobacterial biofilms”
Academic Advisor at SFB/TRR 225: PD Dr. Regina Ebert
Location: University of Wuerzburg, Orthopedic Center for Musculoskeletal Research
Topic of thesis: Glycoengineering as a tool for regulating behaviour of mesenchymal stem cells in biofabrication
Main techniques and methodologies to be used: Cell culture techniques, Click chemistry (bioconjugation), Biochemical assays (proliferation, apoptosis), qPCR (differentiation, senescence, mechanotransduction), Microscopy (differentiation, senescence, viability, stiffness)

Contact: stephan.altmann@klh.de

Jürgen Mut

Previous academic education:  Master of Science
Academic Advisor within SFB/TRR 225: Prof. Dr. Jürgen Seibel
Location: University of Wuerzburg, Institute of Organic Chemistry
Topic of thesis: Synthese von Fluor-substituierten, aromatischen Galektin-1 Inhibitoren
Main techniques and methodologies to be used: Organic synthesis

Contact: juergen.mut@uni-wuerzburg.de

Seibel, J (2018) Glycoengineering as a tool for visualizing and redesigning the human and bacterial cell surfaces, GlykoT 2018, The 11th International Symposium on Glycosyltransferases, 19-23 June 2018 (Qingdao, China)

Altmann S, Mut J, Wolf N, Bechold J, Jakob F, Seibel J, Ebert R (2019) Glycoengineering as a tool to control the behavior of mesenchymal stem cells in biofabrication processes, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)

Julian Bechold

Previous academic education: Master-Thesis
Academic Advisor within SFB/TRR 225: Prof. Dr. Jürgen Seibel
Location: University of Würzburg, Institute of Organic Chemistry
Topic of thesis: Synthesis of Galectin-1-Inhibitors
Main techniques and methodologies to be used: Organic Synthesis, Chromatography, Spectrometry, protein expression

Contact: julian.bechold@uni-wuerzburg.de

Natalia Wolf

Previous academic education:  M. Sc. Chemistry
Academic Advisor within SFB/TRR 225: Prof. Dr. Jürgen Seibel
Location: University of Wuerzburg, Institute of Organic Chemistry
Topic of thesis: Synthesis and application of cyanine based dyes in super resolution microscopy.
Main techniques and methodologies to be used: Organic synthesis, spectroscopy, modification and functionalization of various materials (glas slides, gold chips).

Contact: natalia.wolf@uni-wuerzburg.de