B05 | Glycoengineering as a tool to control the behavior of mesenchymal stem cells in biofabrication processes

The modification of the microenvironment of cells by using click chemistry and synthetic sugar structures represents a new tool for the development of bioinks as well as for the transient modification of cell surfaces. Bioinks will be functionalized by glycoengineering in such a way that cells selectively adhere and differentiate. On the other hand, the extracellular glycocalyx will be modified and/or a protective polysaccharide layer will be added to protect cells from shear forces arising during bioprinting. The long-term goal is to modify cells and bioinks by glycoengineering in such a way that the post-production behavior of cells is optimized in biofabricated constructs.

Prof. Dr. Regina Ebert
Prof. Dr. Jürgen Seibel

Stephan Altmann

Previous academic education: M.Sc. Biochemistry, Topic of thesis: “To be or not to be planktonic? Investigation of single species cyanobacterial biofilms”
Academic Advisor at SFB/TRR 225: PD Dr. Regina Ebert
Location: University of Wuerzburg, Orthopedic Center for Musculoskeletal Research
Topic of thesis: Glycoengineering as a tool for regulating behaviour of mesenchymal stem cells in biofabrication
Main techniques and methodologies to be used: Cell culture techniques, Click chemistry (bioconjugation), Biochemical assays (proliferation, apoptosis), qPCR (differentiation, senescence, mechanotransduction), Microscopy (differentiation, senescence, viability, stiffness)

Contact: stephan.altmann@klh.de

Jürgen Mut

Previous academic education:  Master of Science
Academic Advisor within SFB/TRR 225: Prof. Dr. Jürgen Seibel
Location: University of Wuerzburg, Institute of Organic Chemistry
Topic of thesis: Synthese von Fluor-substituierten, aromatischen Galektin-1 Inhibitoren
Main techniques and methodologies to be used: Organic synthesis

Contact: juergen.mut@uni-wuerzburg.de

Ortiz-Soto ME, Reising S, Schlosser A, Seibel J (2019) Structural and functional role of disulphide bonds and substrate binding residues of the human beta-galactoside alpha-2,3-sialyltransferase 1 (hST3Gal1) Scientific Reports volume 9, Article number: 17993

Ertl J, Ortiz‐Soto ME, Le TA,Bechold J, Shan J, Teßmar J, Engels B, Seibel J (2019) Tuning the Product Spectrum of a Glycoside Hydrolase Enzyme by a Combination of Site-Directed Mutagenesis and Tyrosine-Specific Chemical Modification, Chem. Eur. J. 2019, 25, 1 – 11

Ziouti F*, Ebert R*, Rummler M, Krug M, Müller-Deubert S, Lüdemann M, Jakob F, Willie BM, Jundt F (2019) NOTCH signaling is activated through mechanical strain in human bone marrow-derived mesenchymal stromal cells, Stem Cells International Volume 2019, Article ID 5150634  *equal contribution

Gutmann M, Bechold J, Seibel J, Meinel L, Lühmann T  (2018) Metabolic Glycoengineering of Cell-Derived Matrices and Cell Surfaces: A Combination of Key Principles and Step-by-Step Procedures, ACS Biomater. Sci. Eng., Article ASAP,DOI: 10.1021/acsbiomaterials.8b00865,Publication Date (Web): November 20, 2018

Gutmann M, Braun A, Seibel J, Lühmann T. (2018) Bioorthogonal Modification of Cell Derived Matrices by Metabolic Glycoengineering. ACS Biomater. Sci. Eng. 2018 Mar 13, doi: 10.1021/acsbiomaterials.8b00264.

Ortiz-Soto ME, Ertl J, Mut J, Adelmann J, Le TA, Shan J, Teßmar J, Schlosser A, Engels B, Seibel J. (2018) Product-oriented chemical surface modification of a levansucrase (SacB) via an ene-type reaction., Chem Sci. 2018 May 22;9(24):5312-5321. doi: 10.1039/c8sc01244j. eCollection 2018 Jun 28.   Front Cover Picture for Chemical Science   Press Release

Altmann S, Mut J, Wolf N, Bechold J, Jakob F, Seibel J, Ebert R (2019) Glycoengineering as a tool to control the behavior of bone marrow-derived mesenchymal stromal cells in biofabrication processes, 1st MuSkITYR Symposium 2. – 3.12.2019 (Günzburg, Germany)

Seibel J (2019) Bioorthogonal conjugation reactions for studying biological events, Gordon Research Conference Carbohydrates, Carbohydrate Synthesis and Research to Advance Glycobiology and Biomedicine June 23 – 28, 2019 (Hong Kong, China)

Seibel, J (2018) Glycoengineering as a tool for visualizing and redesigning the human and bacterial cell surfaces, GlykoT 2018, The 11th International Symposium on Glycosyltransferases, 19-23 June 2018 (Qingdao, China)

Altmann S, Mut J, Wolf N, Bechold J, Jakob F, Seibel J, Ebert R (2019) Glycoengineering as a tool to control the behavior of mesenchymal stem cells in biofabrication processes, 22nd European Congress on Alternatives to Animal Testing, 10-13 October 2019 (Linz, Austria)

Altmann S, Mut J, Wolf N, Bechold J, Jakob F, Seibel J, Ebert R (2019) Glycoengineering as a tool to control the behavior of mesenchymal stem cells in biofabrication processes, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); 9-13 September 2019 (Dresden, Germany)

Altmann S, Mut J, Wolf N, Bechold J, Jakob F, Seibel J, Ebert R (2019) Glycoengineering as a tool to control the behavior of mesenchymal stem cells in biofabrication processes, 46th European Calcified Tissue Society Congress, 11-14 May 2019 (Budapest, Hungary)

Julian Bechold

Previous academic education: Master-Thesis
Academic Advisor within SFB/TRR 225: Prof. Dr. Jürgen Seibel
Location: University of Würzburg, Institute of Organic Chemistry
Topic of thesis: Synthesis of Galectin-1-Inhibitors
Main techniques and methodologies to be used: Organic Synthesis, Chromatography, Spectrometry, protein expression

Contact: julian.bechold@uni-wuerzburg.de

Natalia Wolf

Previous academic education:  M. Sc. Chemistry
Academic Advisor within SFB/TRR 225: Prof. Dr. Jürgen Seibel
Location: University of Wuerzburg, Institute of Organic Chemistry
Topic of thesis: Synthesis and application of cyanine based dyes in super resolution microscopy.
Main techniques and methodologies to be used: Organic synthesis, spectroscopy, modification and functionalization of various materials (glas slides, gold chips).

Contact: natalia.wolf@uni-wuerzburg.de