Due to the complexity of bioprinted constructs, the supply of the embedded cells with nutrients, as well as maturation of the construct to a functional tissue, tailored conditions must be maintained. The technological focus of TP B03 is on the integration of the bioprinting process in a functional bioreactor system where the bioconstruct and the bioreactor are printed simultaneously. By biofabrication of tissue models based on hydrogel composite materials and by generation of a suitable bioreactor platform, the feasibility of this concept will be shown. Furthermore, functional biofabrication of muscle tissue will demonstrate for the efficient generation of personalized specific 3D construct.
Previous academic education: Bachelor of Science in Nanotechnology, Master of Science in Nanotechnology – passed with distinction: “Tissue Plasminogen Activator Functionalized Iron Oxide Nanoparticles for Targeted Fibrin Clot Dissolution”
Academic Advisor at SFB/TRR 225: Prof. Dr.-Ing. habil. Aldo R. Boccaccini, Dr. Rainer Detsch (technical advisor)
Location: Friedrich-Alexander University of Erlangen-Nuremberg, Institute of Biomaterials
Topic of thesis: Simultaneous printing of biofabricated constructs and bioreactors: composite bioinks and non-degradable polymers for regenerative approaches
Main techniques and methodologies to be used: Hard and soft printing, mechanical studies, rheology, spectroscopy, microscopy, cell culture
Previous academic education: Diploma thesis
Academic Advisor within SFB/TRR 225: Dr. Sahar Salehi
Location: University of Bayreuth, Department of biomaterials, Faculty of Engineering
Topic of thesis: Simultaneous printing of biofabricated constructs and bioreactors: composite bioinks for regenerative approaches (skeletal muscle tissue)
Main techniques and methodologies to be used: Soft-printing, rheology, mechanical tests, spectroscopy, microscopy and cell culture.
Previous academic education: Master (Biology)
Academic Advisor within SFB/TRR 225: Dr.-Ing. Jan Hansmann
Location: University of Würzburg, Department Tissue Engineering and Regenerative Medicine
Topic of thesis: Simultaneous printing of bioreactor and tissue (working title)
Main techniques and methodologies to be used: In Silico Simulations, Cell Culture, 3D Printing, Bioreactor Handling
Uribe-Gomez J, Posada-Murcia A, Shukla A, Ergin M, Constante G, Apsite I, Dulle M, Schwarzer M, Caspari A, Synytska A, Salehi S, Ionov L (2021) Shape-Morphing Fibrous Hydrogel/Elastomer Bilayers Fabricated by a Combination of 3D Printing and Melt Electrowriting for MuscleTissue Regeneration, ACS Appl. Bio Mater. 2021
Constante G, Apsite I, Alkhamis H, Dulle M, Schwarzer M, Caspari A, Synytska A, Salehi S, Ionov L (2021) 4D biofabrication using a combination of 3D printing and melt-electrowriting of shape-morphing polymers, ACS Applied Materials & Interfaces
Gensler M, Leikeim A, Mollmann M,Komma M, Heid S, Müller C, AldoR.Boccaccini, SaharSalehi, FlorianGroeber-Becker,JanHansmann (2020) 3D printing of bioreactors in tissue engineering: A generalised approach, PLoSONE 15(11):e0242615.
Mueller C, Trujillo-Miranda M, Maier M, Heath DE, O’Connor A, Salehi S (2020) Effects of External Stimulators on Engineered Skeletal Muscle Tissue Maturation, Advanced Materials Interfaces Volume8, Issue1
Apsite I, Constante G, Dulle M, Vogt L, Caspari A, Boccaccini AR, Synytska A, Salehi S, Ionov L (2020) 4D Biofabrication of fibrous artificial nerve graft for neuron regeneration, Biofabrication 12 035027
Russell CS, Mostafavi A, Quint JP, Panayi AC, Baldino K, Williams TJ, Daubendiek JG, Sánchez VH, Bonick Z, Trujillo-Miranda M, Shin SR, Pourquie O, Salehi S, Sinha I, Tamayol A (2020) In Situ Printing of Adhesive Hydrogel Scaffolds for the Treatment of Skeletal Muscle Injuries, ACS Appl. Bio Mater. 2020, 3, 3, 1568–1579
Schwedhelm I, Zdzieblo D, Apelt-Menzel A, Berger C, Schmitz T, Schuldt B, Franke A, Müller FJ, Pless O, Schwarz T, Wiedemann P, Walles H, Hansmann J (2019) Automated real-time monitoring of human pluripotent stem cell aggregation in stirred tank reactors, Scientific Reports volume 9, Article number: 12297
Distler T, Ruther F, Boccaccini AR, Detsch R (2019) Development of 3D Biofabricated Cell Laden Hydrogel Vessels and a Low-Cost Desktop Printed Perfusion Chamber for In Vitro Vessel Maturation, Macromol. Biosci.2019, 1900245
Ostrovidov S, Salehi S, Costantini M, Suthiwanich K, Ebrahimi M, Sadeghian RB, Fujie T, Shi X, Cannata S, Gargioli C, Tamayol A, Dokmeci MR, Orive G, Swieszkowski W, Khademhosseini A (2019) 3D Bioprinting in Skeletal Muscle Tissue Engineering Small. 2019 Apr 23:e1805530
Wicklein VJ, Singer BB, Scheibel T, Salehi S (2018) Nanoengineered biomaterials for corneal regeneration, anoengineered Biomaterials for Regenerative Medicine
A volume in Micro and Nano Technologies – Book Chapter 17; Pages 379-415, ISBN 978-0-12-813355-2 2019 Elsevier Inc.
Steiner D, Lingens L, Fischer L, Köhn K, Detsch R, Boccaccini AR, Fey T, Greil P, Weis C, Beier J, Horch RE, Arkudas A. (2018) Encapsulation of mesenchymal stem cells improves vascularization of alginate-based scaffolds. Tissue Eng Part A. 2018 Mar 20. doi: 10.1089/ten.TEA.2017.0496. Front Cover for Tissue Engineering Part A
Trujillo Miranda M, Müller C, Salehi S (2020) Effect of external stimulus on biofabricated skeletal muscle microtissues, 11th World biomaterials congress WBC 2020 11 – 15 December 2020 — Web conference
Russell CS, Mostafavi A, Quint JP, Panayi A, Udeh K, Williams TJ, Daubendiek JG, Sánchez VH, Bonick Z, Trujill-Miranda M, Ryon Shin S, Pourquie O, Salehi S, Sinha I, Tamayol A (2020) In situ printing of gelatin-based scaffolds for the treatment of muscle injuries, World biomaterials congress WBC 2020 Glasgow 11 – 15 December 2020 – Web conference
Heid S, Lu HH, Venkatraman A, Detsch R, Boccaccini AR (2020) Evaluating the 3D Printaility of ADA-GEL-based Composite Bioinks, Materials Science and Engineering (MSE), 22 – 25 September 2020 – Web conference
Salehi, S (2020) Application of micro/nano scale substrates for skeletal muscle tissue regeneration, 8th International Conference on Nanoscience and Nanotechnology (ICONN 2020) 9 – 13 February 2020, Bisbane, Australia
Detsch R, Hazur J, Karakaya E, Kuth S, Heid S, Boccaccini AR (2019) Evaluating three different extrusion-based bioprinting systems using cell-laden alginate-based bioinks, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)
Trujillo Miranda M, Uribe J, Apsite I, DeSimone E, Salehi S, Ionov L (2019) Fabrication of tubular structure to mimic the osteon structure, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)
Heid S, Detsch R, Boccaccini AR (2019) New Alginate-Based Composite Bioinks for Enhanced 3D Bioprinting; TERMIS EU Workshop 2019, August, 26 to 27 2019, (Nantes, France)
Salehi S (2019) Mimicking the osteon structure by biofabrication techniques, TERMIS European Chapter Meeting 2019 27th-31st May 2019, Rhodes, Greece Tissue Engineering Therapies: From Concept to Clinical Translation & Commercialisation
Salehi S, Kharaziha M, Ionov L, Scheibel T. (2019) Composite ink development from bioceramic nanoparticles for bone tissue regeneration, 9th biennial Australian Colloid and Interface Symposium (ACIS) 3 – 7 February 2019 (Hobart, Tasmania, Australia)
Boccaccini AR (2018) Bioinks containing reactive fillers in biofabrication approaches, Invited presentation at West Pomeranian University of Technology, 22 November 2018 (Szczecin, Poland)
Boccaccini AR (2018) Bioactive Materials and Biofabrication for Biomedical Applications: Progress and Challenges, Invited speaker at Scientific Workshop 2018, Rostock University Medicine (Universitätsmedizin Rostock), 16 November 2018 (Rostock, Germany)
Boccaccini A. (2018) Bioactive materials and biofabrication for tissue engineering: achievements and challenges”, Invited presentation at Polytechnic School (Escuela Politecnica Superior, EPS), University of Seville, 31st October 2018 (Seville, Spain).
Boccaccini A. (2018) Development of composite bioinks incorporating bioreactive inorganic particles, Invited presentation at: International Conference on Biofabrication (ISBF) 2018, 28-31 October 2018 (Würzburg, Germany).
Boccaccini A. (2018) Biofabrication with alginate di-aldehyde / gelatin (ADA/GEL) hydrogels: progress, applications and challenges, Oral presentation at: Biomaterials and Novel Technologies for Healthcare, 2nd International Biennial Conference BioMaH, Frascati, 8-11 October, 2018 (Rome, Italy).
Boccaccini A. (2018) Bioactive materials and biofabrication for tissue engineering applications. Invited seminar at: CONICET Laboratory of Integration of Cellular Signals at the Institute for Histology and Embryology, Faculty of Medicine, National University of Cuyo (UNCUYO), 27th August 2018 (Mendoza, Argentina).
Boccaccini A. (2018) Bioactive materials and biofabrication for bone and soft tissue engineering, Invited lecture at: Justus-Liebig-University Giessen, Medical School, 19th July 2018 (Giessen, Germany).
Boccaccini A. (2018) Bioactive materials and biofabrication for tissue engineering: progress and challenges. Invited speaker at: Junior EUROMAT 2018 Conference, 8-12 July 2018 (Budapest, Hungary).
Boccaccini A. (2018) Enhancing alginate based bioinks for biofabrication: the composites approach, Invited speaker at: 1st TERMIS-EU Workshop on “3D Printing in Musculoskeletal Tissue Engineering”, 22-23 March 2018 (Warsaw, Poland).
Boccaccini A. (2018) Bioactive materials and biofabrication approaches for hard tissue regeneration, Invited speaker at: 2nd Sino-German Symposium on Reconstruction and Repair of Musculoskeletal Tissues, Mainz University Medical Center, 13-14 March 2018 (Mainz, Germany).
Müller C, Salehi S (2020) Fabrication of support structures for 3D bioprinting skeletal muscle cells, World biomaterials congress WBC 2020 Glasgow, 11 – 15 December 2020 – Web conference
Müller C, Gensler M, Heid S, Boccaccini AR, Detsch R, Hansmann J, Salehi S (2020) Simultaneous printing of biofabricates and tailored bioreactors, 5th Bioinspired Materials 2020 Conference, 16 – 19 March 2020 – Web conference
Karakaya E, Hazur J, Teßmar J, Boccaccini AR, Detsch R(2019) How to Determine a Suitable Sodium Alginate for the Fabrication of Standardized Alginatedialdehyde-Gelatin crosslinked Hydrogels in Biofabrication Approaches? Annual Conference of the International Society for Biofabrication (ISBF), 20-22 October 2019 (Columbus, Ohio, USA)
Heid S, Detsch R, Boccaccini AR (2019) Is There an Optimum in the Design of Alginate-Based Composite Bioinks for 3D Bioprinting Regarding the Filler Concentration? Annual Conference of the International Society for Biofabrication (ISBF), 20-22 October 2019 (Columbus, Ohio, USA)
Heid S, Detsch R, Boccaccini AR (2019) Alginate-Based Composite Bioinks for 3D Biofabrication, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)
Salehi S (2019) Electrospun Stimuli-Responsive Mats for Muscle Tissue Regeneration, TeRMIS European Chapter Meeting 2019 27th-31st May 2019, Tissue Engineering Therapies: From Concept to Clinical Translation & Commercialisation (Rhodes, Greece)
Salehi S, Ionov L, Scheibel T (2018) Bioactive Composite Ink for Bone Tissue Regeneration, Annual Conference of the International Society for Biofabrication (ISBF), Würzburg, Germany, on 28-31 October, 2018.
Gensler M, Komma M, Heid S, Detsch R, Salehi S, Boccaccini AR, Hansmann J (2018) Bioreactor concepts for simultaneous printing of bioreactor and tissue equivalent, Annual Conference of the International Society for Biofabrication (ISBF), 28-31 October 2018 (Würzburg, Germany)