A03 | Thermo-gelling poly(2-oxazolin) based hydrogels with temporal mechanical control

This project aims at developing a synthetic, cell-compatible and modular bioink-platform. Diblock copolymers based on poly(2-oxazoline)s will be deployed exhibiting suitable thermo- and shearresponsive behavior. This novel bioink platform will be modified by a dual-crosslinking mechanism to (i) guarantee mechanical stability after printing and (ii) to modulate the stiffness of the ink during cell-culture experiments.

PD Dr. Tessa Lühmann
Prof. Dr. Robert Luxenhofer

Matthias Beudert

Previous academic education: Bachelor of Science in Biochemistry at the Friedrich-Schiller-University Jena, Master of Science in Molecular Biotechnology at the Technical University Munich
Academic Advisor within SFB TRR225: PD Dr. Tessa Lühmann
Location: University of Wuerzburg, Department of Pharmaceutical Technology and Biopharmacy
Topic of thesis: Development of cell compatible bioinks
Main techniques and methodologies to be used: Peptide Synthesis, Polymer Functionalization,  HPLC, Rheology, Cell Culture, Bioprinting, Cytotoxic Assays, FACS, Enzymatic Functionalization

Contact: matthias.beudert@uni-wuerzburg.de

Lukas Hahn

Previous academic education: Biochemistry (BSc, Würzburg), Natural products and drug chemistry (MSc, Bayreuth)
Academic Advisor within SFB TRR225: Prof. Dr. Robert Luxenhofer
Location: University of Würzburg, Lehrstuhl Chemische Technologie der Materialsynthese, Functional Polymer Materials
Topic of thesis: Structure-property relationships in dual-crosslinking poly(2-oxazoline)/poly(2-oxazine) bioinks
Main techniques and methodologies to be used: Monomer synthesis; polymer synthesis, acidic hydrolysis, post-polymerization modification, NMR spectroscopy; GPC, rheological profiling

Contact: lukas.hahn@uni-wuerzburg.de

Zahoranova A, Luxenhofer R, Poly(2-oxazoline)- and Poly(2-oxazine)-Based Self-Assemblies, Polyplexes, and Drug Nanoformulations-An Update, Adv Healthc Mater 10(6) (2021) 2001382. (A03)

Hahn L, Karakaya E, Zorn T, Sochor B, Maier M, Stahlhut P, Forster S, Fischer K, Seiffert S, Poppler AC, Detsch R, Luxenhofer R, An Inverse Thermogelling Bioink Based on an ABA-Type Poly(2-oxazoline) Amphiphile, Biomacromolecules doi:10.1021/acs.biomac.1c00427  (2021). (A03, B06)

Hahn L, Keßler L, Polzin L, Fritze L, Forster S, Helten H, Luxenhofer R, ABA Type Amphiphiles with Poly(2‐benzhydryl‐2‐oxazine) Moieties: Synthesis, Characterization and Inverse Thermogelation, Macromol. Chem. Phys. doi:10.1002/macp.202100114  (2021). (A03)

Zahoranova A, Luxenhofer R, Poly(2-oxazoline)- and Poly(2-oxazine)-Based Self-Assemblies, Polyplexes, and Drug Nanoformulations-An Update, Adv Healthc Mater 10(6) (2021) 2001382. (A03)

Hu C, Hahn L, Yang MS, Altmann A, Stahlhut P, Groll J, Luxenhofer R, Improving printability of a thermoresponsive hydrogel biomaterial ink by nanoclay addition, J. Mater. Sci. 56(1) (2021) 691-705. (A03)

Haider MS, Ahmad T, Yang M, Hu C, Hahn L, Stahlhut P, Groll J, Luxenhofer R, Tuning the Thermogelation and Rheology of Poly(2-Oxazoline)/Poly(2-Oxazine)s Based Thermosensitive Hydrogels for 3D Bioprinting, Gels 7(3) (2021). (A03)

Hahn L, Karakaya E, Zorn T, Sochor B, Maier M, Stahlhut P, Forster S, Fischer K, Seiffert S, Poppler AC, Detsch R, Luxenhofer R, doi:10.1021/acs.biomac.1c00427An Inverse Thermogelling Bioink Based on an ABA-Type Poly(2-oxazoline) Amphiphile, Biomacromolecules  22, 7, 3017–3027  (2021). (A03, B06)

Hahn L, Beudert M, Gutmann M, Kessler L, Stahlhut P, Fischer L, Karakaya E, Lorson T, Thievessen I, Detsch R, Luhmann T, Luxenhofer R, From Thermogelling Hydrogels toward Functional Bioinks: Controlled Modification and Cytocompatible Crosslinking, Macromol. Biosci. doi:10.1002/mabi.202100122  (2021) e2100122. (A03, B06)

Altmann S, Mut J, Wolf N, Meissner-Weigl J, Rudert M, Jakob F, Gutmann M, Luhmann T, Seibel J, Ebert R, Metabolic Glycoengineering in hMSC-TERT as a Model for Skeletal Precursors by Using Modified Azide/Alkyne Monosaccharides, Int. J. Mol. Sci. 22(6) (2021) 2820. (B05, A03)

Lorson T, Ruopp M, Nadernezhad A, Eiber J, Vogel U, Jungst T, Luhmann T, Sterilization Methods and Their Influence on Physicochemical Properties and Bioprinting of Alginate as a Bioink Component, ACS Omega 5(12) (2020) 6481-6486. (A03)

Landrya MR, DuRossa AN, Neufeld MJ, Hahn L, Sahaya G, Luxenhofer R, Suna C (2020) Low Dose Novel PARP-PI3K Inhibition via Nanoformulation Improves Colorectal Cancer, I Materials Today Bio Volume 8, September 2020, 100082(A03)

Hahn L, Maier M, Stahlhut P, Beudert M, Flegler V, Forster S, Altmann A, Toppke F, Fischer K, Seiffert S, Bottcher B, Luhmann T, Luxenhofer R, Inverse Thermogelation of Aqueous Triblock Copolymer Solutions into Macroporous Shear-Thinning 3D Printable Inks, ACS Appl Mater Interfaces 12(11) (2020) 12445-12456. (A03)

Berger C, Bjorlykke Y, Hahn L, Muhlemann M, Kress S, Walles H, Luxenhofer R, Raeder H, Metzger M, Zdzieblo D, Matrix decoded – A pancreatic extracellular matrix with organ specific cues guiding human iPSC differentiation, Biomaterials 244 (2020) 119766. (A03)

Lubtow MM, Mrlik M, Hahn L, Altmann A, Beudert M, Luhmann T, Luxenhofer R, Temperature-Dependent Rheological and Viscoelastic Investigation of a Poly(2-methyl-2-oxazoline)-b-poly(2-iso-butyl-2-oxazoline)-b-poly(2-methyl-2-oxaz oline)-Based Thermogelling Hydrogel, J. Funct. Biomater. 10(3) (2019) 36. (A03)

Lübtow MM, Lorson T, Finger T, Gröber‐Becker FK, Luxenhofer R, Combining Ultra‐High Drug‐Loaded Micelles and Injectable Hydrogel Drug Depots for Prolonged Drug Release, Macromol. Chem. Phys. 221(1) (2019). (A03)

Gutmann M, Bechold J, Seibel J, Meinel L, Luhmann T, Metabolic Glycoengineering of Cell-Derived Matrices and Cell Surfaces: A Combination of Key Principles and Step-by-Step Procedures, ACS Biomater Sci Eng 5(1) (2019) 215-233. Available open access as part of the ACS Editors’ Choice initiative(A03, B05)

Lorson T, Lubtow MM, Wegener E, Haider MS, Borova S, Nahm D, Jordan R, Sokolski-Papkov M, Kabanov AV, Luxenhofer R, Poly(2-oxazoline)s based biomaterials: A comprehensive and critical update, Biomaterials 178 (2018) 204-280. (A03)

Gutmann M, Braun A, Seibel J, Luhmann T, Bioorthogonal Modification of Cell Derived Matrices by Metabolic Glycoengineering, ACS Biomater Sci Eng 4(4) (2018) 1300-1306. (A03, B05)

Hahn L, Maier M, Sochor B, Beudert M, Lühmann T, Luxenhofer R (2019) Poly(2-oxazoline)/Poly(2-oxazine) based Copolymers: Physical gelation and related properties, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)

Luxenhofer R (2018) Pseudo-polypeptides forming thermoresponsive, biocompatible bioinks, International Conference on Biofabrication (ISBF) 2018, 28-31 October 2018 (Würzburg, Germany)

Lühmann T (2018) Nanomechanics of fibroblast growth factor 2 – heparin at physiological and pathophysiological conditions: Implications for drug delivery, Annual meeting of the DPhG. Hamburg, 4-6 October 2018 (Hamburg, Germany)

Luxenhofer R (2018) Pseudo-Polypeptides: From surface modification to drug delivery and 3D bioprinting, Tomas Bata University, 19 September 2018 (Zlín, Czech Republic)

Lühmann T (2018) Tissue painting: Methods and application, Living materials meeting, Tufts University, European centre, 9-12 July 2018 (Talloires, France)

Luxenhofer R (2018) Poly(2-oxazoline) amphiphiles: From ultra-high loaded polymer micelles to 3D bioprinting, Invited speaker at Advanced Functional Polymers for Medicine, 16-18 May 2018 (Montpellier, France)

Lorson T, Jaksch S, Jüngst T, Groll J, Lühmann T, Luxenhofer R (2018)  Nanostructured, thermoresponsive and biocompatible hydrogels based on Poly(2-oxazoline)s for 3D bioprinting and biofabrication, Joint DIPC, JCNS & SoftComp Workshop 2018: Functional Polymers, 19-21 March 2018 (San Sebastian, Spain)

Hahn L, Beudert M, Lorson T, Lühmann T, Luxenhofer R (2020) Poly(2-oxazoline)/Poly(2-oxazine) copolymers: From thermoresponsive hydrogels towards functional bioink formulations, 5th Bioinspired Materials 2020 Conference, 16 – 19 March 2020 – Web conference

Hahn L, Beudert M, Lorson T, Lühmann T, Luxenhofer R (2019) Post-polymerization modification of thermogelling poly(2-oxazoline)/poly(2-oxazine) based hydrogels ensuring biocompatibility and temporal mechanical control, European Polymer Federation (EPF) Congress 9-14 June 2019 (Hersonissos, Crete, Greece)

Beudert M, Hahn L, Lorson T, Deiwiks A, Luxenhofer R, Lühmann T (2018) Implementation of cell adhesion in thermogelling poly(2-oxazoline)-based hydrogels for their use as bioinks in bioprinting 6th Galenus Workshop.  6-8 November (Würzburg, Germany)

Hahn L, Beudert M, Lorson T, Lühmann T, Luxenhofer R (2020) Poly(2-oxazoline)/Poly(2-oxazine) copolymers: From thermoresponsive hydrogels towards functional bioink formulations, 5th Bioinspired Materials 2020 Conference, 16 – 19 March 2020 – Web conference

Beudert M, Hahn L, Lorson T, Deiwiks A, Luxenhofer R, Lühmann T (2018) Biofunctionalization of thermogelling poly(2-oxazoline)-based hydrogels by Diels Alder chemistry, Annual Conference of the International Society for Biofabrication (ISBF), 28-31 October, 2018 (Würzburg, Germany)

Hahn L, Hüttner N, Deiwiks A, Lorson T, Lühmann T, Luxenhofer R (2018) Poly-(2-methyl-2-oxazoline) and thermoresponsive poly(-2-n-propyl-oxazine): Different architecture same viscoelastic profile?, Annual Conference of the International Society for Biofabrication (ISBF), 28-31 October 2018 (Würzburg, Germany)

Lorson T, Jüngst T, Jaksch S, Lühmann S, Luxenhofer R. (2018) Biocompatible,Thermoresponsive, and Printable Poly(2-Oxazoline) Gels. Polymers: Design, Function and Application, Barcelona, 22–23 Mar 2018

Dr. Thomas Lorson

Previous academic education: Master Thesis
Academic Advisor within SFB TRR225:  PD Dr. Tessa Lühmann
Location: University of Würzburg, Department of Pharmaceutical Technology and Biopharmacy
Topic of thesis: Novel polymeric biomaterials and scaffold designs for tissue engineering applications
Main techniques and methodologies to be used: Polymer Synthesis, Modification & Characterization, Rheology, Bioprinting

Contact: thomas.lorson@uni-wuerzburg.de