A03 | Thermo-gelling poly(2-oxazolin) based hydrogels with temporal mechanical control

This project aims at developing a synthetic, cell-compatible and modular bioink-platform. Diblock copolymers based on poly(2-oxazoline)s will be deployed exhibiting suitable thermo- and shearresponsive behavior. This novel bioink platform will be modified by a dual-crosslinking mechanism to (i) guarantee mechanical stability after printing and (ii) to modulate the stiffness of the ink during cell-culture experiments.

PD Dr. Tessa Lühmann
Prof. Dr. Robert Luxenhofer

Matthias Beudert

Previous academic education: Bachelor of Science in Biochemistry at the Friedrich-Schiller-University Jena, Master of Science in Molecular Biotechnology at the Technical University Munich
Academic Advisor within SFB TRR225: PD Dr. Tessa Lühmann
Location: University of Wuerzburg, Department of Pharmaceutical Technology and Biopharmacy
Topic of thesis: Development of cell compatible bioinks
Main techniques and methodologies to be used: Peptide Synthesis, Polymer Functionalization,  HPLC, Rheology, Cell Culture, Bioprinting, Cytotoxic Assays, FACS, Enzymatic Functionalization

Contact: matthias.beudert@uni-wuerzburg.de

Lukas Hahn

Previous academic education: Biochemistry (BSc, Würzburg), Natural products and drug chemistry (MSc, Bayreuth)
Academic Advisor within SFB TRR225: Prof. Dr. Robert Luxenhofer
Location: University of Würzburg, Lehrstuhl Chemische Technologie der Materialsynthese, Functional Polymer Materials
Topic of thesis: Structure-property relationships in dual-crosslinking poly(2-oxazoline)/poly(2-oxazine) bioinks
Main techniques and methodologies to be used: Monomer synthesis; polymer synthesis, acidic hydrolysis, post-polymerization modification, NMR spectroscopy; GPC, rheological profiling

Contact: lukas.hahn@uni-wuerzburg.de

Dr. Thomas Lorson

Previous academic education: Master Thesis
Academic Advisor within SFB TRR225:  PD Dr. Tessa Lühmann
Location: University of Würzburg, Department of Pharmaceutical Technology and Biopharmacy
Topic of thesis: Novel polymeric biomaterials and scaffold designs for tissue engineering applications
Main techniques and methodologies to be used: Polymer Synthesis, Modification & Characterization, Rheology, Bioprinting

Contact: thomas.lorson@uni-wuerzburg.de

Hahn L, Maier M, Stahlhut P, Beudert M, Flegler V, Forster S, Altmann A, Töppke F, Fischer K, Seiffert S, Böttcher B, Lühmann T, Luxenhofer R (2020) Inverse Thermogelation of Aqueous Triblock Copolymer Solutionsinto Macroporous Shear-Thinning 3D Printable Inks, ACS Applied Materials & Interfaces

Bergera C, Bjørlykke Y, Hahn L, Mühlemann M, Kressa S, Walles H, Luxenhofer R, Ræder H, Metzger M, Zdziebloae D (2020) Matrix decoded – A pancreatic extracellular matrix with organ specific cues guiding human iPSC differentiation, Biomaterials

Hahn L, Maier M, Stahlhut P, Beudert M, Altmann A,  Töppke F, Lühmann T, Luxenhofer R (2019) Inverse Thermogelation of Aqueous Triblock Copolymer Solutions into Macroporous Shear-Thinning 3D Printable Inks, ChemRxiv. Preprint.

Lübtow MM, Lorson T, Finger T, Gröber-Becker FK, Luxenhofer R (2019) Combining ultra-high drug loaded micelles and shear-thinning, injectable hydrogel drug depots for prolonged drug-release. ChemRxiv 2019

Lübtow MM, Mrlik M, Hahn L, Altmann A, Beudert M, Lühmann T and Luxenhofer R (2019) Temperature-Dependent Rheological and Viscoelastic Investigation of a Poly(2-methyl-2-oxazoline)-b-poly(2-iso-butyl-2-oxazoline)-b-poly(2-methyl-2-oxazoline)-Based Thermogelling Hydrogel, J. Funct. Biomater. 2019, 10(3), 36

Gutmann M, Bechold J, Seibel J, Meinel L, Lühmann T (2018) Metabolic Glycoengineering of Cell-Derived Matrices and Cell Surfaces: A Combination of Key Principles and Step-by-Step Procedures, ACS Biomater. Sci. Eng., Article ASAP,DOI: 10.1021/acsbiomaterials.8b00865,Publication Date (Web): November 20, 2018   Available open access as part of the ACS Editors’ Choice initiative

Lorson T, Lübtow MM, Wegener E, Haider MS, Borova S, Nahm D, Jordan R, Sokolski-Papkov M, Kabanov AV, Luxenhofer R (2018) Poly(2-oxazoline)s based biomaterials: A comprehensive and critical update. Biomaterials. 2018 Sep;178:204-280. doi: 10.1016/j.biomaterials.2018.05.022

Gutmann M, Braun A, Seibel J, Lühmann T. (2018) Bioorthogonal Modification of Cell Derived Matrices by Metabolic Glycoengineering. ACS Biomater. Sci. Eng. 2018 Mar 13, doi: 10.1021/acsbiomaterials.8b00264.

Hahn L, Maier M, Sochor B, Beudert M, Lühmann T, Luxenhofer R (2019) Poly(2-oxazoline)/Poly(2-oxazine) based Copolymers: Physical gelation and related properties, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)

Luxenhofer R (2018) Pseudo-polypeptides forming thermoresponsive, biocompatible bioinks, International Conference on Biofabrication (ISBF) 2018, 28-31 October 2018 (Würzburg, Germany)

Lühmann T (2018) Nanomechanics of fibroblast growth factor 2 – heparin at physiological and pathophysiological conditions: Implications for drug delivery, Annual meeting of the DPhG. Hamburg, 4-6 October 2018 (Hamburg, Germany)

Luxenhofer R (2018) Pseudo-Polypeptides: From surface modification to drug delivery and 3D bioprinting, Tomas Bata University, 19 September 2018 (Zlín, Czech Republic)

Lühmann T (2018) Tissue painting: Methods and application, Living materials meeting, Tufts University, European centre, 9-12 July 2018 (Talloires, France)

Luxenhofer R (2018) Poly(2-oxazoline) amphiphiles: From ultra-high loaded polymer micelles to 3D bioprinting, Invited speaker at Advanced Functional Polymers for Medicine, 16-18 May 2018 (Montpellier, France)

Lorson T, Jaksch S, Jüngst T, Groll J, Lühmann T, Luxenhofer R (2018)  Nanostructured, thermoresponsive and biocompatible hydrogels based on Poly(2-oxazoline)s for 3D bioprinting and biofabrication, Joint DIPC, JCNS & SoftComp Workshop 2018: Functional Polymers, 19-21 March 2018 (San Sebastian, Spain)

Hahn L, Beudert M, Lorson T, Lühmann T, Luxenhofer R (2019) Post-polymerization modification of thermogelling poly(2-oxazoline)/poly(2-oxazine) based hydrogels ensuring biocompatibility and temporal mechanical control, European Polymer Federation (EPF) Congress 9-14 June 2019 (Hersonissos, Crete, Greece)

Beudert M, Hahn L, Lorson T, Deiwiks A, Luxenhofer R, Lühmann T (2018) Implementation of cell adhesion in thermogelling poly(2-oxazoline)-based hydrogels for their use as bioinks in bioprinting 6th Galenus Workshop.  6-8 November (Würzburg, Germany)

Beudert M, Hahn L, Lorson T, Deiwiks A, Luxenhofer R, Lühmann T (2018) Biofunctionalization of thermogelling poly(2-oxazoline)-based hydrogels by Diels Alder chemistry, Annual Conference of the International Society for Biofabrication (ISBF), 28-31 October, 2018 (Würzburg, Germany)

Hahn L, Hüttner N, Deiwiks A, Lorson T, Lühmann T, Luxenhofer R (2018) Poly-(2-methyl-2-oxazoline) and thermoresponsive poly(-2-n-propyl-oxazine): Different architecture same viscoelastic profile?, Annual Conference of the International Society for Biofabrication (ISBF), 28-31 October 2018 (Würzburg, Germany)

Lorson T, Jüngst T, Jaksch S, Lühmann S, Luxenhofer R. (2018) Biocompatible,Thermoresponsive, and Printable Poly(2-Oxazoline) Gels. Polymers: Design, Function and Application, Barcelona, 22–23 Mar 2018