A01 | Alginate based Hydrogels with adhesive functionalization and controlled degradation

The aim of subproject A01 is the systematic investigation and optimization of adhesive, microstructural as well as microscopic and macroscopic mechanical properties of biofabricated hierarchical tissue analogues based on alginate dialdehyde (ADA). This also includes optimizing the temporal degradation of the alginate matrix and its replacement by a cell-generated extracellular matrix. Our long-term goal is to achieve tight control over the temporal changes in adhesive, mechanical and structural properties and the associated cell-biological behavior in alginate-based biofabricated tissues.

Prof. Dr. Aldo Boccaccini
Prof. Dr. Ben Fabry

Jonas Hazur

Previous academic education: Sc. in Nanotechnology, Sc. in Materials Science: “Characterization of rheological behavior and processability in additive manufacturing of different medical grade alginate gel systems“
Academic Advisor within SFB TRR225: Prof. Dr.-Ing. habil. Aldo R. Boccaccini, Dr.-Ing. Rainer Detsch (technical advisor)
Location: Friedrich-Alexander University of Erlangen-Nuremberg, Institute of Biomaterials
Topic of thesis: Systematical investigation of adhesive functionalized and controlled degrading alginate based hydrogels
Main techniques and methodologies to be used: Bioplotting, chemical and physical modification, cell culture, degradation studies, spectroscopy, microscopy, rheology

Contact: Jonas.hazur@fau.de

Huijun Zhang

Previous academic education: M. Sc. Polymer Chemistry
Academic Advisor within SFB TRR225: Prof. Dr. Ben Fabry
Location: Friedrich-Alexander University of Erlangen-Nuremberg, Biophysics group, Center for Medical Physics and Technology
Topic of thesis: Development and characterization of organic-inorganic hybrids and composites for tissue engineering
Main techniques and methodologies to be used: Bioplotting, chemical and physical modification, spectroscopy, microscopy, rheology, degradation studies, cell migration assays
Contact: huijun.zhang@fau.de

Spörrer M, Kah D, Gerum RC, Reischl B, Huraskin D, Dessalles CA, Schneider W, Goldmann WH, Herrmann H, Thievessen I, Clemen CS, Friedrich O, Hashemolhosseini S, Schröder R, Fabry B, The desmin mutation R349P increases contractility and fragility of stem cell-generated muscle micro-tissues, bioRxiv (2021) 2021.07.23.453481 (A01, B08, C02)

Hazur J, Endrizzi N, Schubert DW, Boccaccini AR, Fabry B, Stress relaxation amplitude of hydrogels determines migration, proliferation, and morphology of cells in 3-D, bioRxiv (2021)2021.07.08.451608 (A01, A07, C02)

Bauer A, Prechova M, Fischer L, Thievessen I, Gregor M, Fabry B, pyTFM: A tool for traction force and monolayer stress microscopy, PLoS Comput Biol 17(6) (2021) e1008364. (A01, B06)

Bednarzig V, Karakaya E, Egana AL, Teßmar J, Boccaccini AR, Detsch R, Advanced ADA-GEL bioink for bioprinted artificial cancer models, Bioprinting doi:10.1016/j.bprint.2021.e00145  (2021) 00145. (B06, A01, A02)

Fuentes-Chandia M, Vierling A, Kappelmann-Fenzl M, Monavari M, Letort G, Hone L, Parma B, Antara SK, Ertekin O, Palmisano R, Dong M, Bopple K, Boccaccini AR, Ceppi P, Bosserhoff AK, Leal-Egana A, 3D Spheroids Versus 3D Tumor-Like Microcapsules: Confinement and Mechanical Stress May Lead to the Expression of Malignant Responses in Cancer Cells, Adv. Biol. doi:10.1002/adbi.202000349  (2021) 2000349. (C03, A01)

Genc H, Hazur J, Karakaya E, Dietel B, Bider F, Groll J, Alexiou C, Boccaccini AR, Detsch R, Cicha I, Differential Responses to Bioink-Induced Oxidative Stress in Endothelial Cells and Fibroblasts, Int. J. Mol. Sci. 22(5) (2021) 2358. (B02, A01, B06)

Kah D, Winterl A, Přechová M, Schöler U, Schneider W, Friedrich O, Gregor M, Fabry B, A low-cost uniaxial cell stretcher for six parallel wells, HardwareX 9 (2021). (A01, B08, C02)

Distler T, Solisito AA, Schneidereit D, Friedrich O, Detsch R, Boccaccini AR, 3D printed oxidized alginate-gelatin bioink provides guidance for C2C12 muscle precursor cell orientation and differentiation via shear stress during bioprinting, Biofabrication 12(4) (2020) 045005. (A01, B06, B08)

Dranseikiene D, Schrufer S, Schubert DW, Reakasame S, Boccaccini AR, Cell-laden alginate dialdehyde-gelatin hydrogels formed in 3D printed sacrificial gel, J. Mater. Sci.: Mater. Med. 31(3) (2020) 31. (A01, A07)

Hazur J, Detsch R, Karakaya E, Kaschta J, Tessmar J, Schneidereit D, Friedrich O, Schubert DW, Boccaccini AR, Improving alginate printability for biofabrication: establishment of a universal and homogeneous pre-crosslinking technique, Biofabrication 12(4) (2020) 045004. (A01, A02, A07, B06, B08)

Karakaya E, Fischer L, Hazur J, Boccaccini AR, Thievessen I, Detsch R, Strategies to evaluate alginate based bioinks applying extrusion printingfor biofabrication, Additive Manufacturing Meets Medicine, 2020. (B06, A01)

Mirzaei Z, Kuth S, Schubert DW, Detsch R, Roether JA, Blunk T, Kordestani SS, Boccaccini AR, Preparation and Characterization of Electrospun Blend Fibrous Polyethylene Oxide:Polycaprolactone Scaffolds to Promote Cartilage Regeneration, Adv. Eng. Mater. 22(9) (2020). (A01, A02, A07, B06)

Muller SJ, Mirzahossein E, Iftekhar EN, Bacher C, Schrufer S, Schubert DW, Fabry B, Gekle S, Flow and hydrodynamic shear stress inside a printing needle during biofabrication, PLoS One 15(7) (2020) 0236371. (B07, A01, A07)

Rausch M, Bohringer D, Steinmann M, Schubert DW, Schrufer S, Mark C, Fabry B, Measurement of Skeletal Muscle Fiber Contractility with High-Speed Traction Microscopy, Biophys. J. 118(3) (2020) 657-666. (A01, A07)

Reimann R, Kah D, Mark C, Dettmer J, Reimann TM, Gerum RC, Geitmann A, Fabry B, Dietrich P, Kost B, Durotropic Growth of Pollen Tubes, Plant Physiol. 183(2) (2020) 558-569. (A01, C02)

Schmid R, Schmidt SK, Hazur J, Detsch R, Maurer E, Boccaccini AR, Hauptstein J, Tessmar J, Blunk T, Schrufer S, Schubert DW, Horch RE, Bosserhoff AK, Arkudas A, Kengelbach-Weigand A, Comparison of Hydrogels for the Development of Well-Defined 3D Cancer Models of Breast Cancer and Melanoma, Cancers 12(8) (2020) 2320. (C03, A01, A02, A07, C02)

Distler T, Ruther F, Boccaccini AR, Detsch R, Development of 3D Biofabricated Cell Laden Hydrogel Vessels and a Low-Cost Desktop Printed Perfusion Chamber for In Vitro Vessel Maturation, Macromol. Biosci. 19(9) (2019) 1900245. (B06, A01, B03)

Rottensteiner-Brandl U, Detsch R, Sarker B, Lingens L, Kohn K, Kneser U, Bosserhoff AK, Horch RE, Boccaccini AR, Arkudas A, Encapsulation of Rat Bone Marrow Derived Mesenchymal Stem Cells in Alginate Dialdehyde/Gelatin Microbeads with and without Nanoscaled Bioactive Glass for In Vivo Bone Tissue Engineering, Materials (Basel) 11(10) (2018) 1880. (C04, A01, C03)

Ruther F, Distler T, Boccaccini AR, Detsch R, Biofabrication of vessel-like structures with alginate di-aldehyde-gelatin (ADA-GEL) bioink, J. Mater. Sci.: Mater. Med. 30(1) (2018) 8. (B06, A01)

Steiner D, Lingens L, Fischer L, Kohn K, Detsch R, Boccaccini AR, Fey T, Greil P, Weis C, Beier JP, Horch RE, Arkudas A, Encapsulation of Mesenchymal Stem Cells Improves Vascularization of Alginate-Based Scaffolds, Tissue Eng Part A 24(17-18) (2018) 1320-1331. (C03, C04, A01, B06, B03) Front Cover for Tissue Engineering Part A

Karakya E, Fischer L, Hazur J, Boccaccini ARB, Thievessen I, Detsch R (2020) Strategies to evaluate alginate based bioinks applyingextrusion printing for biofabrication, AMMM – Additive Manufacturing Meets Medicine 2020, 9 – 11 September 2020 – Web conference

Hazur J, Geske M, Esper L, Boccaccini  AR (2020) Tailoring mechanical properties and degradation behavior of alginate dialdehyde-gelatin (ADA-GEL) hydrogels, 5th Bioinspired Materials 2020 Conference, 16 – 19 March 2020 – Web conference

Hazur J, Kaschta J, Schubert DW, Detsch R, Boccaccini AR (2019) Rheologische Charakterisierung und 3D Biodrucken von vorvernetzten Alginat Biotinten, WeekstoffWoche Dresden; September 18 to 20, 2019 (Dresden, Germany)

Detsch R, Hazur J, Karakaya E, Kuth S, Heid S, Boccaccini AR (2019) Evaluating three different extrusion-based bioprinting systems using cell-laden alginate-based bioinks, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)

Boccaccini AR (2018) Bioinks containing reactive fillers in biofabrication approaches, Invited presentation at West Pomeranian University of Technology, 22 November 2018 (Szczecin, Poland)

Boccaccini AR (2018) Bioactive Materials and Biofabrication for Biomedical Applications: Progress and Challenges, Invited speaker at Scientific Workshop 2018, Rostock University Medicine (Universitätsmedizin Rostock), 16 November 2018 (Rostock, Germany)

Boccaccini AR (2018) Bioactive materials and biofabrication for tissue engineering: achievements and challenges”, Invited presentation at Polytechnic School (Escuela Politecnica Superior, EPS), University of Seville, 31st October 2018 (Seville, Spain).

Boccaccini AR (2018) Development of composite bioinks incorporating bioreactive inorganic particles, Invited presentation at: International Conference on Biofabrication (ISBF) 2018, 28-31 October 2018 (Würzburg, Germany).

Boccaccini AR (2018) Biofabrication with alginate di-aldehyde / gelatin (ADA/GEL) hydrogels: progress, applications and challenges, Oral presentation at: Biomaterials and Novel Technologies for Healthcare, 2nd International Biennial Conference BioMaH, Frascati, 8-11 October, 2018 (Rome, Italy).

Boccaccini AR (2018) Bioactive materials and biofabrication for tissue engineering applications. Invited seminar at: CONICET Laboratory of Integration of Cellular Signals at the Institute for Histology and Embryology, Faculty of Medicine, National University of Cuyo (UNCUYO), 27th August 2018 (Mendoza, Argentina).

Boccaccini AR (2018) Bioactive materials and biofabrication for bone and soft tissue engineering, Invited lecture at: Justus-Liebig-University Giessen, Medical School, 19th July 2018 (Giessen, Germany).

Boccaccini AR (2018) Bioactive materials and biofabrication for tissue engineering: progress and challenges. Invited speaker at: Junior EUROMAT 2018 Conference, 8-12 July 2018 (Budapest, Hungary).

Boccaccini AR (2018) Enhancing alginate based bioinks for biofabrication: the composites approach, Invited speaker at: 1st TERMIS-EU Workshop on “3D Printing in Musculoskeletal Tissue Engineering”, 22-23 March 2018 (Warsaw, Poland).

Boccaccini AR (2018) Bioactive materials and biofabrication approaches for hard tissue regeneration, Invited speaker at: 2nd Sino-German Symposium on Reconstruction and Repair of Musculoskeletal Tissues, Mainz University Medical Center, 13-14 March 2018 (Mainz, Germany).

Schneidereit D, Distler T, Meinert C, Carlé B, Detsch R, Schürmann S, Hutmacher D, Boccaccini AR, Friedrich O (2020) Multiphoton Microscopy: A powerful tool to reveal cellular organization and morphology within bioengineered constructs in 3D, 5th Bioinspired Materials 2020 Conference, 16 – 19 March 2020 – Web conference

Karakaya E, Hazur J, Teßmar J, Boccaccini AR, Detsch R(2019) How to Determine a Suitable Sodium Alginate for the Fabrication of Standardized Alginatedialdehyde-Gelatin crosslinked Hydrogels in Biofabrication Approaches? Annual Conference of the International Society for Biofabrication (ISBF), 20-22 October 2019 (Columbus, Ohio, USA)

Hazur J, Kaschta J, Schubert DW, Detsch R, Boccaccini AR (2019) Rheological examintation and 3D bioprinting of internally pre-crosslinked alginate bioinks, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)

Detsch R, Boccaccini AR (2018) Advances in Biomaterials for Biofabrication: 3D Printing with Bioinks, “Tag der Technischen Fakultät” of the FAU Erlangen, 16 November 2018 (Erlangen, Germany)

Sebastian Richter

Previous academic education: Dipl. Ing.
Academic Advisor within SFB TRR225: Prof. Dr. Ben Fabry
Location: Friedrich-Alexander University of Erlangen-Nuremberg, LS f Biophysik
Topic of thesis:
Main techniques and methodologies to be used: Software- & Hardware-Development / Mashine Learning / Computer Vision

Contact: srichter@biomed.uni-erlangen.de

Elham Mirzahossein

Previous academic education: Master of Physics in Sharif University of Technology
Academic Advisor within SFB TRR225: Prof. Dr. Ben Fabry
Location: Friedrich-Alexander University of Erlangen-Nuremberg, Department of Physics
Topic of thesis: Adhesive functionalized and controlled degrading alginate-based hydrogels
Main techniques and methodologies to be used: 3D- printer, fluorescent microscopy, microfluidic and micro-indentor

Contact: Elham.mirzahossein@fau.de