A01 | Alginate based Hydrogels with adhesive functionalization and controlled degradation

The aim of subproject A01 is the systematic investigation and optimization of adhesive, microstructural as well as microscopic and macroscopic mechanical properties of biofabricated hierarchical tissue analogues based on alginate dialdehyde (ADA). This also includes optimizing the temporal degradation of the alginate matrix and its replacement by a cell-generated extracellular matrix. Our long-term goal is to achieve tight control over the temporal changes in adhesive, mechanical and structural properties and the associated cell-biological behavior in alginate-based biofabricated tissues.

Prof. Dr. Aldo Boccaccini
Prof. Dr. Ben Fabry

Jonas Hazur

Previous academic education: Sc. in Nanotechnology, Sc. in Materials Science: “Characterization of rheological behavior and processability in additive manufacturing of different medical grade alginate gel systems“
Academic Advisor within SFB TRR225: Prof. Dr.-Ing. habil. Aldo R. Boccaccini, Dr.-Ing. Rainer Detsch (technical advisor)
Location: Friedrich-Alexander University of Erlangen-Nuremberg, Institute of Biomaterials
Topic of thesis: Systematical investigation of adhesive functionalized and controlled degrading alginate based hydrogels
Main techniques and methodologies to be used: Bioplotting, chemical and physical modification, cell culture, degradation studies, spectroscopy, microscopy, rheology

Contact: Jonas.hazur@fau.de

Elham Mirzahossein

Previous academic education: Master of Physics in Sharif University of Technology
Academic Advisor within SFB TRR225: Prof. Dr. Ben Fabry
Location: Friedrich-Alexander University of Erlangen-Nuremberg, Department of Physics
Topic of thesis: Adhesive functionalized and controlled degrading alginate-based hydrogels
Main techniques and methodologies to be used: 3D- printer, fluorescent microscopy, microfluidic and micro-indentor

Contact: Elham.mirzahossein@fau.de

Hazur J, Detsch R, Karakaya E, Kaschta J, Tessmar J, Schneidereit D, Friedrich O, Schubert D, Boccaccini AR (2020) Improving alginate printability for biofabrication: establishment of a universal and homogeneous pre-crosslinking technique, Biofabrication in press

Mirzaei Z, Kuth S, Schubert DW, Detsch R, Roether J, Blunk T, Kordestani SS, Boccaccini AR (2020) Preparation and Characterization of Electrospun Blend Fibrous Polyethylene Oxide:Polycaprolactone Scaffolds to Promote Cartilage Regeneration, Advanced Engineering Materials.

Dranseikiene, D., Schrüfer, S., Schubert, D.W., Reakasame S, Boccaccini AR (2020) Cell-laden alginate dialdehyde–gelatin hydrogels formed in 3D printed sacrificial gel, J Mater Sci: Mater Med 31, 31 (2020).

Rausch M, Böhringer D, Steinmann M, Schubert DW, Schrüfer S, Mark C, Fabry B (2019) Measurement of skeletal muscle fiber contractility with high-speed traction microscopy. bioRxiv (2019): 733451. 

Distler T, Ruther F, Boccaccini AR, Detsch R (2019) Development of 3D Biofabricated Cell Laden Hydrogel Vessels and a Low-Cost Desktop Printed Perfusion Chamber for In Vitro Vessel Maturation, Macromol. Biosci.2019, 1900245

Ruther F, Distler T, Boccaccini AR, Detsch R (2018) Biofabrication of vessel-like structures with alginate di-aldehyde—gelatin (ADA-GEL) bioink, J Mater Sci Mater Med. 2018 Dec 29;30(1):8. doi: 10.1007/s10856-018-6205-7. January 2019, 30:8

Rottensteiner-Brandl U, Detsch R, Sarker B, Lingens L, Köhn K, Kneser U, Bosserhoff AK, Horch RE, Boccaccini AR, Arkudas A. (2018) Encapsulation of Rat Bone Marrow Derived Mesenchymal Stem Cells in Alginate Dialdehyde/Gelatin Microbeads with and without Nanoscaled Bioactive Glass for In Vivo Bone Tissue Engineering. Materials (Basel). 2018 Oct 1;11(10). pii: E1880. doi: 10.3390/ma11101880.

Steiner D, Lingens L, Fischer L, Köhn K, Detsch R, Boccaccini AR, Fey T, Greil P, Weis C, Beier J, Horch RE, Arkudas A. (2018) Encapsulation of mesenchymal stem cells improves vascularization of alginate-based scaffolds. Tissue Eng Part A. 2018 Mar 20. doi: 10.1089/ten.TEA.2017.0496.      Front Cover for Tissue Engineering Part A

Hazur J, Geske M, Esper L, Boccaccini  AR (2020) Tailoring mechanical properties and degradation behavior of alginate dialdehyde-gelatin (ADA-GEL) hydrogels, 5th Bioinspired Materials 2020 Conference, 16 – 19 March 2020 – Web conference

Hazur J, Kaschta J, Schubert DW, Detsch R, Boccaccini AR (2019) Rheologische Charakterisierung und 3D Biodrucken von vorvernetzten Alginat Biotinten, WeekstoffWoche Dresden; September 18 to 20, 2019 (Dresden, Germany)

Detsch R, Hazur J, Karakaya E, Kuth S, Heid S, Boccaccini AR (2019) Evaluating three different extrusion-based bioprinting systems using cell-laden alginate-based bioinks, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)

Boccaccini AR (2018) Bioinks containing reactive fillers in biofabrication approaches, Invited presentation at West Pomeranian University of Technology, 22 November 2018 (Szczecin, Poland)

Boccaccini AR (2018) Bioactive Materials and Biofabrication for Biomedical Applications: Progress and Challenges, Invited speaker at Scientific Workshop 2018, Rostock University Medicine (Universitätsmedizin Rostock), 16 November 2018 (Rostock, Germany)

Boccaccini AR (2018) Bioactive materials and biofabrication for tissue engineering: achievements and challenges”, Invited presentation at Polytechnic School (Escuela Politecnica Superior, EPS), University of Seville, 31st October 2018 (Seville, Spain).

Boccaccini AR (2018) Development of composite bioinks incorporating bioreactive inorganic particles, Invited presentation at: International Conference on Biofabrication (ISBF) 2018, 28-31 October 2018 (Würzburg, Germany).

Boccaccini AR (2018) Biofabrication with alginate di-aldehyde / gelatin (ADA/GEL) hydrogels: progress, applications and challenges, Oral presentation at: Biomaterials and Novel Technologies for Healthcare, 2nd International Biennial Conference BioMaH, Frascati, 8-11 October, 2018 (Rome, Italy).

Boccaccini AR (2018) Bioactive materials and biofabrication for tissue engineering applications. Invited seminar at: CONICET Laboratory of Integration of Cellular Signals at the Institute for Histology and Embryology, Faculty of Medicine, National University of Cuyo (UNCUYO), 27th August 2018 (Mendoza, Argentina).

Boccaccini AR (2018) Bioactive materials and biofabrication for bone and soft tissue engineering, Invited lecture at: Justus-Liebig-University Giessen, Medical School, 19th July 2018 (Giessen, Germany).

Boccaccini AR (2018) Bioactive materials and biofabrication for tissue engineering: progress and challenges. Invited speaker at: Junior EUROMAT 2018 Conference, 8-12 July 2018 (Budapest, Hungary).

Boccaccini AR (2018) Enhancing alginate based bioinks for biofabrication: the composites approach, Invited speaker at: 1st TERMIS-EU Workshop on “3D Printing in Musculoskeletal Tissue Engineering”, 22-23 March 2018 (Warsaw, Poland).

Boccaccini AR (2018) Bioactive materials and biofabrication approaches for hard tissue regeneration, Invited speaker at: 2nd Sino-German Symposium on Reconstruction and Repair of Musculoskeletal Tissues, Mainz University Medical Center, 13-14 March 2018 (Mainz, Germany).

Schneidereit D, Distler T, Meinert C, Carlé B, Detsch R, Schürmann S, Hutmacher D, Boccaccini AR, Friedrich O (2020) Multiphoton Microscopy: A powerful tool to reveal cellular organization and morphology within bioengineered constructs in 3D, 5th Bioinspired Materials 2020 Conference, 16 – 19 March 2020 – Web conference

Karakaya E, Hazur J, Teßmar J, Boccaccini AR, Detsch R(2019) How to Determine a Suitable Sodium Alginate for the Fabrication of Standardized Alginatedialdehyde-Gelatin crosslinked Hydrogels in Biofabrication Approaches? Annual Conference of the International Society for Biofabrication (ISBF), 20-22 October 2019 (Columbus, Ohio, USA)

Hazur J, Kaschta J, Schubert DW, Detsch R, Boccaccini AR (2019) Rheological examintation and 3D bioprinting of internally pre-crosslinked alginate bioinks, 30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)

Detsch R, Boccaccini AR (2018) Advances in Biomaterials for Biofabrication: 3D Printing with Bioinks, “Tag der Technischen Fakultät” of the FAU Erlangen, 16 November 2018 (Erlangen, Germany)

Sebastian Richter

Previous academic education: Dipl. Ing.
Academic Advisor within SFB TRR225: Prof. Dr. Ben Fabry
Location: Friedrich-Alexander University of Erlangen-Nuremberg, LS f Biophysik
Topic of thesis:
Main techniques and methodologies to be used: Software- & Hardware-Development / Mashine Learning / Computer Vision

Contact: srichter@biomed.uni-erlangen.de