A02 | Hyaluronic acid based hydrogel platform with multi-functional cross-linkers for the controlled differentiation of mesenchymal stem cells

Hyaluronic acid (HyA) represents a major component of the human extracellular matrix, however, so far highly versatile HyA-based materials for biofabrication are lacking. The overall aim of this project is, to develop a HyA-based bioink platform utilizing branched multi-functional cross-linkers (PEG, polyglycidol) that enables the controlled differentiation of mesenchymal stem cells. The modification with peptides and growth factors will add further biofunctionality to the printed 3D constructs in order to improve chondrogenic and adipogenic differentiation of the stem cells. For the long term, this work aims to generate bioinks for the development of clinically relevant tissue models.

Prof. Dr. Torsten Blunk
Dr. habil. Jörg Teßmar

Leonard Forster

Previous academic education: Bachelor Thesis – 2015; Master Thesis – 2017
Academic Advisor within SFB/TRR 225:  PD Dr. Jörg Teßmar
Location: University of Würzburg, Department for Functional Materials in Medicine and Dentistry
Topic of thesis: Hyaluronic acid based bioinks for bio-fabrication of mesenchymal stem cells
Main techniques and methodologies to be used: Synthesis, Gelation, Rheometry, 3D Bioprinting

Contact: leonard.forster@fmz.uni-wuerzburg.de

Julia Hauptstein

Previous academic education: Biochemistry M. Sc.
Academic Advisor within SFB/TRR 225:  Prof. Dr. Torsten Blunk
Location: University Hospital Würzburg, Department of Trauma, Hand, Plastic and Reconstructive Surgery
Topic of thesis: Hyaluronic Acid Hydrogels for Biofabrication to control Differentiation of Mesenchymal Stem Cells
Main techniques and methodologies to be used: 2D/3D Tissue Culture, Histology, IHC, Microscopy, qRT-PCR, quantitative DNA- and ECM-Assays, FACS, Western Blotting

Contact: Hauptstein_J@ukw.de

Stefanie Schmidt

Previous academic education: Biochemistry M. Sc.
Academic Advisor within SFB/TRR 225:  Prof. Dr. Torsten Blunk
Location: University Hospital Würzburg, Department of Trauma, Hand, Plastic and Reconstructive Surgery
Topic of thesis: Analysis of cell behavior in different 3D hydrogel models for chondrogenic differentiation.
Main techniques and methodologies to be used: 2D/3D Tissue Culture, Histology, IHC, Microscopy, qRT-PCR, quantitative DNA- and ECM-Assays, FACS, Western Blotting

Contact: Schmidt_S5@ukw.de

Horder H, Guaza Lasheras M, Grummel N, Nadernezhad A, Herbig J, Ergun S, Tessmar J, Groll J, Fabry B, Bauer-Kreisel P, Blunk T, Bioprinting and Differentiation of Adipose-Derived Stromal Cell Spheroids for a 3D Breast Cancer-Adipose Tissue Model, Cells 10(4) (2021) 803. (C02, A02, B02, B04)

Blum C, Taskin MB, Shan J, Schilling T, Schlegelmilch K, Tessmar J, Groll J, Appreciating the First Line of the Human Innate Immune Defense: A Strategy to Model and Alleviate the Neutrophil Elastase-Mediated Attack toward Bioactivated Biomaterials, Small 17(13) (2021) 2007551. (A06, A02)

Bednarzig V, Karakaya E, Egana AL, Teßmar J, Boccaccini AR, Detsch R, Advanced ADA-GEL bioink for bioprinted artificial cancer models, Bioprinting doi:10.1016/j.bprint.2021.e00145  (2021) 00145. (B06, A01, A02)

Muir VG, Qazi TH, Shan J, Groll J, Burdick JA, Influence of Microgel Fabrication Technique on Granular Hydrogel Properties, ACS Biomater Sci Eng doi:10.1021/acsbiomaterials.0c01612  (2021). (A02, A06)

Schmidt S, Abinzano F, Mensinga A, Tessmar J, Groll J, Malda J, Levato R, Blunk T, Differential Production of Cartilage ECM in 3D Agarose Constructs by Equine Articular Cartilage Progenitor Cells and Mesenchymal Stromal Cells, Int. J. Mol. Sci. 21(19) (2020) 7071. (A02)

Schmid R, Schmidt SK, Hazur J, Detsch R, Maurer E, Boccaccini AR, Hauptstein J, Tessmar J, Blunk T, Schrufer S, Schubert DW, Horch RE, Bosserhoff AK, Arkudas A, Kengelbach-Weigand A, Comparison of Hydrogels for the Development of Well-Defined 3D Cancer Models of Breast Cancer and Melanoma, Cancers 12(8) (2020) 2320. (C03, A01, A02, A07, C02)

Nadernezhad A, Forster L, Netti F, Adler-Abramovich L, Tessmar J, Groll J, Rheological analysis of the interplay between the molecular weight and concentration of hyaluronic acid in formulations of supramolecular HA/FmocFF hybrid hydrogels, Polym. J. 52(8) (2020) 1007-1012. (A02, B04)

Mirzaei Z, Kuth S, Schubert DW, Detsch R, Roether JA, Blunk T, Kordestani SS, Boccaccini AR, Preparation and Characterization of Electrospun Blend Fibrous Polyethylene Oxide:Polycaprolactone Scaffolds to Promote Cartilage Regeneration, Adv. Eng. Mater. 22(9) (2020). (A01, A02, A07, B06)

Mancini IAD, Schmidt S, Brommer H, Pouran B, Schafer S, Tessmar J, Mensinga A, van Rijen MHP, Groll J, Blunk T, Levato R, Malda J, van Weeren PR, A composite hydrogel-3D printed thermoplast osteochondral anchor as example for a zonal approach to cartilage repair: in vivo performance in a long-term equine model, Biofabrication 12(3) (2020) 035028. (A02)

Levato R, Jungst T, Scheuring RG, Blunk T, Groll J, Malda J, From Shape to Function: The Next Step in Bioprinting, Adv. Mater. 32(12) (2020) 1906423. (A02, B04, Z01)

Hazur J, Detsch R, Karakaya E, Kaschta J, Tessmar J, Schneidereit D, Friedrich O, Schubert DW, Boccaccini AR, Improving alginate printability for biofabrication: establishment of a universal and homogeneous pre-crosslinking technique, Biofabrication 12(4) (2020) 045004. (A01, A02, A07, B06, B08)

Hauptstein J, Bock T, Bartolf-Kopp M, Forster L, Stahlhut P, Nadernezhad A, Blahetek G, Zernecke-Madsen A, Detsch R, Jungst T, Groll J, Tessmar J, Blunk T, Hyaluronic Acid-Based Bioink Composition Enabling 3D Bioprinting and Improving Quality of Deposited Cartilaginous Extracellular Matrix, Adv Healthc Mater 9(15) (2020) 2000737. (A02, A06, B02, B06, Z01)

Frischholz S, Berberich O, Bock T, Meffert RH, Blunk T, Resveratrol counteracts IL-1beta-mediated impairment of extracellular matrix deposition in 3D articular chondrocyte constructs, J. Tissue Eng. Regener. Med. 14(7) (2020) 897-908. (A02)

Weis M, Shan J, Kuhlmann M, Jungst T, Tessmar J, Groll J, Evaluation of Hydrogels Based on Oxidized Hyaluronic Acid for Bioprinting, Gels 4(4) (2018) 82. (A02)

Bock T, Schill V, Krahnke M, Steinert AF, Tessmar J, Blunk T, Groll J, TGF-beta1-Modified Hyaluronic Acid/Poly(glycidol) Hydrogels for Chondrogenic Differentiation of Human Mesenchymal Stromal Cells, Macromol. Biosci. 18(7) (2018) 1700390. (A02)

Hauptstein J, Böck T, Bartolf-Kopp M, Forster L, Stahlhut P, Nadernezhad A, Blahetek G, Zernecke-Madsen A, Detsch R, Jüngst T, Groll J, Teßmar J, Blunk T (2020) Hyaluronic Acid-based Bioink Composition Enabling 3D Bioprinting and Improving Quality of Deposited Cartilaginous Extracellular Matrix, Materials Science and Engineering (MSE), 22 – 25 September 2020 – Web conference

Hauptstein J, Bartolf-Kopp M, Forster L, Detsch R, Jüngst T, Groll J, Teßmar J, Blunk T (2020) Hyaluronic Acid-based Bioink Composition Enabling 3D Bioprinting and Improving Quality of Deposited Cartilaginous Extracellular Matrix, 5th Bioinspired Materials 2020 Conference, 16 – 19 March 2020 – Web conference

Tessmar J (2020) 3D-Druck von Organen -Fiktion oder Wirklichkeit? 18.02.2020 Universitätsbund Würzburg Marktheidenfeld, im Raum 3.1 des Alten Rathauses, Marktplatz 24

Shan J, Böck TC, Tessmar J, Groll J (2019) EMPO Oxidation of Hyaluronic Acid for Stable Hydrogel Formation via Schiff Base Chemistry,30th Annual Conference of the European Society for Biomaterials in tandem with the 26thAnnual Conference of the German Society for Biomaterials (DGBM); September 9 to 13, 2019 (Dresden, Germany)

Jüngst T, Tessmar J, Dalton P, Groll J (2019) Bionik Development and Melt Electrowriting, Deutsches Zentrum für Luft-und Raumfahrt in Bonn, Workshop Additive Fertigung in der Raumfahrt. 28 May 2019 (Bonn, Germany)

Forster L, Hauptstein J, Weichhold J, Nadernezhad A, Böhm TM, Zorn T, Schmidt S, Blunk T, Teßmar J (2020) Hyaluronic Acid based Hydrogel Platform with MultifuctionalCrosslinkers for the Controlled Differentiation of Mesenchymal Stem Cells, 5th Bioinspired Materials 2020 Conference, 16 – 19 March 2020 – Web conference

Karakaya E, Hazur J, Teßmar J, Boccaccini AR, Detsch R(2019) How to Determine a Suitable Sodium Alginate for the Fabrication of Standardized Alginatedialdehyde-Gelatin crosslinked Hydrogels in Biofabrication Approaches? Annual Conference of the International Society for Biofabrication (ISBF), 20-22 October 2019 (Columbus, Ohio, USA)

Schmidt S, Mancini IAD, Boeck T, Abinzano F, Mensinga F, Hauptstein J, Tessmar J, van Weeren PR , Malda J, Groll J, Levato R, Blunk T (2019) EUREKA! Symposium, 9th and 10th October, 2019, Rudolf Virchow Center, (Würzburg, Germany)

Schmidt S, Mancini IAD, Boeck T, Abinzano F, Mensinga A, Martini J, Tessmar J, van Weeren PR, Malda J, Groll J, Levato R, Blunk T (2018) Chondrogenic Differentiation of Mesenchymal Stem Cells and Articular Cartilage Progenitor Cells in a 3D Printable Hyaluronic Acid Hydrogel, Annual Conference of the International Society for Biofabrication (ISBF), 28-31 October 2018 (Würzburg, Germany).

Leonard Forster, Julia Hauptstein, Ali Nadernezhad, Jürgen Groll, Torsten Blunk, Jörg Tessmar

Hyaluron-based dual-stage crosslinking approach for 3D bioprinting of mesenchymal stem cells

11TH WORLD BIOMATERIALS CONGRESS 11 – 15 December 2020

https://wbc2020.org/

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